Cycloprodigiosin hydrochloride, a H+/Cl- symporter, induces apoptosis in human colon cancer cell lines in vitro

Yamamoto, Chizuko; Takemoto, Hiroto; Kuno, Kenji; Yamamoto, Daigo; Nakai, Koji; Baden, Tetsuya; Kamata, Keiko; Hirata, Hajime; Watanabe, Toshihiko; Inoue, Katsumi

doi:10.3892/or.8.4.821

Cited by 10 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5, 6). Since PG analogues especially prodigiosin and cycloprodigiosin hydrochloride have been shown to induce apoptosis in several cancer cells, 6,12,14,16,25) the apoptosis-induction may be a common cytotoxic mechanism of PG analogues. Apoptosis is known as programmed cell death, and intracellular signal transduction is generally considered to be involved.…”

Section: Discussionmentioning

confidence: 99%

“…[6][7][8][9][10][11] Although the exact cytotoxic mechanisms of PG family members are not fully clarified yet, apoptotic mechanism has been proposed for some PG analogues. For instance, it has been reported that cycloprodigiosin hydrochloride induces apoptosis in cancer cells both in vitro and in vivo, with high efficiency in liver cancer, breast cancer cell lines, and colon cancer cells, [12][13][14] with no apparent toxicity to normal cells. 14) PG produced by S. marcescens also induces apoptosis in haematopoietic and gastrointestinal cancer cell lines, but no marked toxicity to non-malignant cell lines.…”

mentioning

confidence: 99%

“…For instance, it has been reported that cycloprodigiosin hydrochloride induces apoptosis in cancer cells both in vitro and in vivo, with high efficiency in liver cancer, breast cancer cell lines, and colon cancer cells, [12][13][14] with no apparent toxicity to normal cells. 14) PG produced by S. marcescens also induces apoptosis in haematopoietic and gastrointestinal cancer cell lines, but no marked toxicity to non-malignant cell lines. 7,15,16) Considering the high selectivity of PG family members to cancer cells, these pigments may be promising candidates for new anticancer drugs with unique action mechanism.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by .GAMMA.-Proteobacterium and Its Multiple Bioactivities

Nakashima

Tamura

Kurachi

et al. 2005

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

A bacterial strain (MS-02-063), which produces red pigment, was discovered from our library established with more than 20000 microorganisms isolated from coastal area of Nagasaki prefecture, Japan. Comparison of 16S rDNA gene sequence of strain MS-02-063 with sequences from GenBank demonstrated that this strain belongs to the g-proteobacterium species. Recent our study has demonstrated that the highly purified red pigment showed potent antimicrobial activity against pathogenic microorganisms such as Staphylococcus aureus and dermatophytes. 1)Prodigiosin (PG) analogues belonging to a family of natural red pigments are characterized by a common pyrrolydipyrrolylmethene skeleton structure with a C-4 methoxy group, and several PG-like pigments have been isolated from certain microorganisms 2-4) including some genera of actinomycetes, Pseudoalteromonas denitrificans, and Serratia marcescens. Representative members of PG analogues are prodigiosin, undecylprodigiosin, cycloprodigiosin hydrochloride, metacycloprodigiosin, and nonylprodigiosin. 5) Some of PG analogues have been reported to have several biological activities such as immunosuppressive, antimalarial, antimicrobial and cytotoxic activities. [6][7][8][9][10][11] Although the exact cytotoxic mechanisms of PG family members are not fully clarified yet, apoptotic mechanism has been proposed for some PG analogues. For instance, it has been reported that cycloprodigiosin hydrochloride induces apoptosis in cancer cells both in vitro and in vivo, with high efficiency in liver cancer, breast cancer cell lines, and colon cancer cells, [12][13][14] with no apparent toxicity to normal cells. 14) PG produced by S. marcescens also induces apoptosis in haematopoietic and gastrointestinal cancer cell lines, but no marked toxicity to non-malignant cell lines. 7,15,16) Considering the high selectivity of PG family members to cancer cells, these pigments may be promising candidates for new anticancer drugs with unique action mechanism. Regarding the structure-activity relationship, it has been reported that a nitrogen-containing heterocyclic A-pyrrole ring with the lone-pair nitrogen electrons in conjugation with the tricyclic frame and a C-4 alkoxy are important for the biological activities of PG analogues. 17)Although there are extensive studies on the cytotoxicities of PG analogues produced by S. marcescens and P. denitrificans, 4,16) only limited information on the bioactivities of PG analogues produced by g-proteobacterium is available. In this study, we examined the cytotoxic effects of PG analogue (PG-L-1), produced by g-proteobacterium (strain MS-02-063) on various cultured cell lines. Since U937 cells showed the highest susceptibility to PG-L-1 cytotoxicity, we conducted detailed analysis of cytotoxic mechanism of PG-L-1 in U937 cells especially in terms of apoptotic cell death. To search for new aspect of biochemical activity of PG-L-1, we also examined the effect of PG-L-1 on the O 2 Ϫ generation by 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated U937 cells. ...

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by .GAMMA.-Proteobacterium and Its Multiple Bioactivities

Nakashima

Tamura

Kurachi

et al. 2005

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…In HL-60 cells, cycloprodigiosin HCl (300 nM) triggered apoptosis in 20% of the cells and differentiation in 60% of the cells [77]. The ability of cycloprodigiosin HCl to induce apoptosis was also observed in human hepatocellular carcinoma cell lines [78], breast cancer cell lines [79], and colon cancer cell lines [80]. Microcolin A is a lipopeptide first isolated from a Venezuelan cyanobacteria Lyngbya majuscula [81].…”

Section: Apoptosismentioning

confidence: 99%

Mechanism Targeted Discovery of Antitumor Marine Natural Products

Nagle¹,

Zhou²,

Mora³

et al. 2004

Current Medicinal Chemistry

View full text Add to dashboard Cite

Antitumor drug discovery programs aim to identify chemical entities for use in the treatment of cancer. Many strategies have been used to achieve this objective. Natural products have always played a major role in anticancer medicine and the unique metabolites produced by marine organisms have increasingly become major players in antitumor drug discovery. Rapid advances have occurred in the understanding of tumor biology and molecular medicine. New insights into mechanisms responsible for neoplastic disease are significantly changing the general philosophical approach towards cancer treatment. Recently identified molecular targets have created exciting new means for disrupting tumor-specific cell signaling, cell division, energy metabolism, gene expression, drug resistance, and blood supply. Such tumor-specific treatments could someday decrease our reliance on traditional cytotoxicity-based chemotherapy and provide new less toxic treatment options with significantly fewer side effects. Novel molecular targets and state-of-the-art molecular mechanism-based screening methods have revitalized antitumor research and these changes are becoming an ever-increasing component of modern antitumor marine natural products research. This review describes marine natural products identified using tumor-specific mechanism-based assays for regulators of angiogenesis, apoptosis, cell cycle, macromolecule synthesis, mitochondrial respiration, mitosis, multidrug efflux, and signal transduction. Special emphasis is placed on natural products directly discovered using molecular mechanism-based screening.

show abstract

“…It has been reported that PGs mainly target cancer cells (hematopoietic, gastrointestinal, breast, and lung cancer) with little or no effects on normal cells (16)(17)(18). Numerous studies indicated that PGs displayed selective cytotoxicity against cancer cells, anti-metastatic activity, and p53-independent proapoptotic effects, which points to their potency as anticancer agents (16,19).…”

mentioning

confidence: 99%

The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro

Petrović

Vasic

Mitrović

et al. 2017

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in γ-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to γ-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.

show abstract

Cycloprodigiosin hydrochloride, a H+/Cl- symporter, induces apoptosis in human colon cancer cell lines in vitro

Cited by 10 publications

References 0 publications

Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by .GAMMA.-Proteobacterium and Its Multiple Bioactivities

Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by .GAMMA.-Proteobacterium and Its Multiple Bioactivities

Mechanism Targeted Discovery of Antitumor Marine Natural Products

The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro

Contact Info

Product

Resources

About