Cyclophosphamide Modulates Gene Expression in Neonatal Rat Testis Following Antenatal Exposure to Fetuses During Testicular Differentiation

Saxena, Ashish; Singh, Gajendra

doi:10.1080/014850199262878

Cited by 2 publications

(1 citation statement)

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“…The size of cellular contents of the seminiferous tubule altered significantly since primary=secondary spermatocytes fail to replicate their DNA due to inhibition of an essential enzyme (dihydrofolate reductase) required for normal synthesis of DNA [10], which led to mitotic delay, abnormal protein synthesis and cell enlargement. Although over-expression of 74,500 KD protein was observed in testicular tissue after anticancer drug administration [12]. The mechanism of action of cytotoxic agents result cell-death has not been established.…”

Section: Discussionmentioning

confidence: 99%

Effect of Chronic Low Dose of Methotrexate on Cellular Proliferation During Spermatogenesis in Rats

Saxena

Dhungel

Bhattacharya

et al. 2004

Archives of Andrology

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This study was conducted to evaluate cellular proliferation of germinal and non-germinal elements of seminiferous tubules following continuous Day 1 to Day 17 exposure of methotrexate (12.5 microgram) in male rats. There was significant decrease in the diameter of seminiferous tubules (P < 0.10) followed by increase of interstitial space (P < 0.01). The size of various stages of primary, secondary spermatocytes, and spermatids was altered significantly compared to controls. Vacuolization/decondensation of "chromatin-mass" in spermatocytes changed from rounded to oval. The size of the Sertoli and Leydig cells were reduced significantly. Basement membrane at some places seems to be disrupted and thin in experimental testis. Methotrexate induced cytotoxicity on the proliferation of cellular contents of seminiferous tubules elucidating the mechanism of dose-dependent drug induced testicular damage during spermatogenesis.

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Section: Discussionmentioning

confidence: 99%