Cyclopeptide Alkaloids from Discaria chacaye (Rhamnaceae) as Result of Symbiosis with Frankia (Actinomycetales)

Abstract

Cyclopeptide alkaloids with different biological activities are present in plants of the family Rhamnaceae. Plants of this family grow in a symbiotic relationship with aerobic Gram-positive actinomycetes belonging to the genus Frankia. This goal of this research was a study of the comparative profile of alkaloids present in Discaria chacaye and to establish a connection between the presence or absence of Frankia sp. and the alkaloids. In addition, insecticidal activities of the alkaloidal extract were examined. A total of 24 alkaloids were identified, of which 12 have a benzylisoquinoline skeleton, 9 were cyclopeptides, 2 isoquinolines, and 1 aporphine. The presence of cyclopeptide alkaloids is associated with Frankia nodules in the plant root. The alkaloid extracts showed insecticidal activity with mortality dose-dependence and LD 50 values between 44 to 71 μg/mL.

Impact of conservation tillage on wheat performance and its microbiome

“…These genera belong to Actinomycetales or Pseudomonadales orders, which are well known to colonize roots of different crops such as tomato and wheat ( Aleklett et al., 2022 ; Zhang et al., 2022 ). Furthermore, Streptomyces and Pseudomonas have been described for their plant growth-promoting potential ( Romano et al., 2020 ; Chouyia et al., 2022 ; Quiroz-Carreño et al., 2022 ). Cystofilobasidium , Basidioascus , and Sporobolomyces were the most prevalent fungal 1 genera in the rhizosphere; Sporobolomyces is one of the major fungal genera commonly identified in the wheat rhizosphere ( Levi et al., 2022 ).…”