Cyclooxygenases in Human and Mouse Skin and Cultured Human Keratinocytes: Association of COX-2 Expression with Human Keratinocyte Differentiation

Leong, Jane; Hughes‐Fulford, Millie; Rakhlin, Nina; Habib, Aı̈da; Maclouf, Jacques; Goldyne, Marc E.

doi:10.1006/excr.1996.0113

Cited by 123 publications

(118 citation statements)

References 8 publications

(12 reference statements)

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“…Vogt et al [35] were not able to detect the COX-2 protein in any of 11 cases of BCC, but observed medium and/or strong expression of the protein in nine of 17 cases of SCC and weak staining in two of six keratoacanthomas (KA). The staining pattern observed for cells of the normal skin was similar to that described by Leong et al [34]. In the study by Putti et al [36], COX-2 expression was detected in 13 of 17 SCCs and eight of 24 KAs.…”

Section: Introduction Introduction Introduction Introduction Introducsupporting

confidence: 84%

“…Immunohistochemical studies of COX-2 expression in human cutaneous epithelial tumors and in normal skin reported to date have yielded quite inconsistent data. Leong et al [34] found strong COX-2 expression in squamous cell carcinomas (SCC), while the cells of basal cell carcinomas (BCC) displayed a weak staining. The authors also detected the COX-2 expression of normal skin, but restricted to keratinocytes of the granular and spinous layers.…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

See 1 more Smart Citation

Different expression of cyclooxygenase-2 (COX-2) in selected nonmelanocytic human cutaneous lesions

Kuźbicki¹,

Lange²,

Stanek-Widera³

et al. 2011

Folia Histochem Cytobiol.

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Section: Introduction Introduction Introduction Introduction Introducsupporting

confidence: 84%

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

Different expression of cyclooxygenase-2 (COX-2) in selected nonmelanocytic human cutaneous lesions

Kuźbicki¹,

Lange²,

Stanek-Widera³

et al. 2011

Folia Histochem Cytobiol.

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“…14 Expression of COX-2, however, has not been extensively studied in hematological malignancy, although recent studies suggest that it is also highly expressed outside the gastrointestinal tract. [15][16][17] Here we investigated the role of COX-2 gene expression in the pathogenesis of HTLV-I-associated diseases. We examined COX-2 mRNA expression in human T-cell lines with or without HTLV-I.…”

mentioning

confidence: 99%

Constitutive expression of the cyclooxygenase-2 gene in T-cell lines infected with human T cell leukemia virus type I

Mori

Inoue

Yoshida³

et al. 2001

Int. J. Cancer

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Cyclooxygenase-2 (COX-2), an inducible enzyme that catalyzes the formation of prostaglandins and other eicosanoids from arachidonic acid, is constitutively expressed in several human carcinomas. COX-2 expression, however, has not been extensively studied in leukemia. Human T cell leukemia virus type I (HTLV-I) is the etiologic agent of adult T cell leukemia, an aggressive form of human T cell malignancy. We studied COX-2 mRNA expression in various human Tcell lines. Northern blot analysis revealed that COX-2 mRNA steady-state levels were high in 4 of 7 T-cell lines infected with HTLV-I. COX-2 mRNA, however, was not expressed in any of 3 HTLV-I-negative T-cell lines. We also confirmed COX-2 expression in 6 of 7 HTLV-I-positive T-cell lines by reverse transcription-PCR. HTLV-I Tax is known to increase the expression of cellular genes and thus we assayed Tax for its ability to increase transcription from the COX-2 promoter. Although Tax increased transcription of the COX-2 promoter in a T-cell line, Tax expression did not induce COX-2 mRNA expression, indicating that Tax alone is not sufficient for significant accumulation of COX-2 mRNA, which probably requires an additional viral protein. To evaluate the potential role of COX-2 in T cells, the HTLV-Iinfected T-cell lines were treated with NS398, a selective COX-2 inhibitor. NS398 treatment inhibited proliferation and induced apoptosis of HTLV-I-infected T-cell lines and downregulated Bcl-2 and Bcl-x L mRNA expression, followed by chromosomal DNA fragmentation. Our data suggest that COX-2 is expressed selectively in T-cell lines infected with HTLV-I and that this gene may play a role in cell survival.

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“…Furthermore, PGE 2 is involved in keratinocyte proliferation and differentiation, and this has direct consequences for the epidermal barrier function [25,30,31]. It has also been suggested that fibroblast-produced PGE 2 influences keratinocyte growth showing the cross-talk and biochemical support between skin layers [32,33]. Finally, PGE 2 can act as keratinocyte chemoattractant and modulator of dermal fibroblasts, and in this way can facilitate wound healing [34,35].…”

Section: Cyclooxygenase-derived Mediatorsmentioning

confidence: 99%

Eicosanoids in skin inflammation

Nicolaou

2013

Prostaglandins, Leukotrienes and Essential Fatty Acids

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SummaryEicosanoids play an integral part in homeostatic mechanisms related to skin health and structural integrity. They also mediate inflammatory events developed in response to environmental factors, such as exposure to ultraviolet radiation, and inflammatory and allergic disorders, including psoriasis and atopic dermatitis. This review article discusses biochemical aspects related to cutaneous eicosanoid metabolism, the contribution of these potent autacoids to skin inflammation and related conditions, and considers the importance of nutritional supplementation with bioactives such as omega-3 and omega-6 polyunsaturated fatty acids and plant-derived antioxidants as means of addressing skin health issues.2

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Cyclooxygenases in Human and Mouse Skin and Cultured Human Keratinocytes: Association of COX-2 Expression with Human Keratinocyte Differentiation

Cited by 123 publications

References 8 publications

Different expression of cyclooxygenase-2 (COX-2) in selected nonmelanocytic human cutaneous lesions

Different expression of cyclooxygenase-2 (COX-2) in selected nonmelanocytic human cutaneous lesions

Constitutive expression of the cyclooxygenase-2 gene in T-cell lines infected with human T cell leukemia virus type I

Eicosanoids in skin inflammation

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