Cyclooxygenase-2 Expression Increases with the Stage and Grade in Transitional Cell Carcinoma of the Urinary Bladder

Wadhwa, Pankaj; Goswami, Anil Kumar; Joshi, Kusum; Sharma, Surinder K.

doi:10.1007/s11255-004-4699-z

Cited by 44 publications

(46 citation statements)

References 31 publications

(26 reference statements)

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“…Our previous study showed that COX-2 expression was closely related to the invasion of TCCs and was marked at the invasive front of tumors [22]. This was supported by several previous studies [18][19][20][21]. Here, we showed that abnormal E-cadherin expression was more frequently observed in invasive TCCs.…”

Section: Discussionsupporting

confidence: 88%

“…Prostaglandin E 2 (PGE 2 ) is related to carcinogenesis through immunosuppression, inhibiting apoptosis, increasing the metastatic potential of epithelial cells, and promoting angiogenesis [14,15]. COX-2 overexpression was observed in various tumors as well as in bladder TCCs [16][17][18][19][20][21]. Our previous study has reported that COX-2 overexpression in bladder TCCs was significantly associated with high grade and invasion of tumors [22].…”

Section: Introductionmentioning

confidence: 99%

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Reciprocal correlation between the expression of cyclooxygenase-2 and E-cadherin in human bladder transitional cell carcinomas

2010

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Carcinoma cells become more motile and invasive via downmodulation of E-cadherin. Cyclooxygenase-2 (COX-2) expression is associated with tumor invasion and metastasis. The aim of this study is to investigate the relationship between the expression of COX-2 and E-cadherin in a bladder cancer cell line and human bladder transitional cell carcinoma (TCCs). Phorbol 12-myristate 13-acetate (PMA) treatment for 5637 bladder cancer cells increased COX-2 expression, slightly induced Slug expression, and decreased E-cadherin expression. Ectopic expression of COX-2 or prostaglandin E(2) (PGE(2)) treatment for 5637 cells reduced E-cadherin expression. This finding was confirmed by the result that knockdown of COX-2 expression or indomethacin administration increased the expression of E-cadherin. When compared with cells' motility in serum-free medium, the treatment of PMA and PGE(2) increased cell motility, and indomethacin treatment slightly decreased cell motility. In the tissues of bladder TCCs, COX-2 expression was inversely correlated with membranous E-cadherin expression and positively correlated with nuclear beta-catenin expression. The expression of COX-2 and nuclear beta-catenin expression was significantly higher in TCCs of high grade and invasive growth than in TCCs of low grade and noninvasive growth. In contrast, membranous E-cadherin expression was more decreased in tumors of high grade and invasive growth. In addition, nuclear beta-catenin expression was significantly related to tumor recurrence. We suggest that COX-2 pathway reduces membranous E-cadherin expression in bladder TCCs and their expression pattern may provide important information in predicting the clinical behavior of bladder TCCs.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Reciprocal correlation between the expression of cyclooxygenase-2 and E-cadherin in human bladder transitional cell carcinomas

2010

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“…9,11,23,[54][55][56] Numerous studies have shown significant correlations between COX-2 expression and tumor grade, infiltration, metastasis, and survival. 21,24,30,40,[44][45][46]53 Concordantly, a decreased risk for urinary bladder cancer development has been seen in humans undergoing long-term nonsteroidal anti-inflammatory therapy, and in vitro and in vivo studies have suggested potential use of COX-2 inhibitors in treatment. 3,5,10,29,53 In humans and dogs, COX-2 is not expressed by normal urothelium of the urinary bladder.…”

Section: Introductionmentioning

confidence: 99%

Differences in Expression of Uroplakin III, Cytokeratin 7, and Cyclooxygenase-2 in Canine Proliferative Urothelial Lesions of the Urinary Bladder

et al. 2014

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The expression of immunohistochemical markers that have been used in diagnosis and/or prognostication of urothelial tumors in humans (uroplakin III [UPIII], cytokeratin 7 [CK7], cyclooxygenase-2 [COX-2], and activated caspase 3) was evaluated in a series of 99 canine proliferative urothelial lesions of the urinary bladder and compared to the lesion classification and grade as defined by the World Health Organization / International Society of Urologic Pathology consensus system. There were significant associations between tumor classification and overall UPIII pattern (P ¼ 1.49 Â 10 -18 ), loss of UPIII (P ¼ 1.27 Â 10 -4 ), overall CK7 pattern (P ¼ 4.34 Â 10 -18 ), and COX-2 pattern (P ¼ 8.12 Â 10 -25). In addition, there were significant associations between depth of neoplastic cell infiltration into the urinary bladder wall and overall UPIII pattern (P ¼ 1.54 Â 10 ). There were no significant associations between tumor classification or infiltration and caspase 3 expression pattern.Keywords dog, urinary bladder, oncology, immunohistochemistry, transitional cell carcinoma, uroplakin III, neoplasia, cytokeratin Proliferative lesions of the urothelium of the canine urinary bladder range from benign polyps and papillomas to carcinomas with varying metastatic potential. Also, inflammatory conditions such as polypoid cystitis can form tumor-like masses within the urinary bladder that can be confused with urothelial neoplasms. Classification of urothelial proliferative lesions and histologic grading of urothelial carcinomas is key to accurate prognostication and treatment selection. A classification and grading scheme based on the 1986 World Health Organization (WHO) classification scheme for human urinary bladder and urethral cancer was proposed in 1995 for use in evaluating urothelial neoplasms in dogs based on pattern of growth, nuclear atypia, and degree of infiltration into the urinary bladder wall. 50 In the initial description of this system's use in dogs, significant correlations were found between tumor grade and depth of infiltration, the presence of metastases, and survival time. However, a subsequent smaller study failed to find a significant correlation between grade and prognosis. 41 To our knowledge, this scheme is not widely used in dogs and has been replaced in human medicine by more modern classification schemes. There remains a need for a clear, reproducible, and well-accepted classification and grading scheme for urothelial proliferative lesions in dogs.Currently, the most widely accepted scheme for classification and grading of proliferative urothelial lesions in humans is the WHO / International Society of Urologic Pathology (ISUP) consensus classification system, published in 1998 and updated in 2004. 6,7 In multiple studies, the WHO/ISUP consensus classification system has been demonstrated to be significantly associated with clinical outcome. 2,[32][33][34]37,42,43,51,59 It has long been known that canine urothelial neoplasms are similar to urothelial neoplasms in humans in terms o...

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“…Curcumin also downregulates NF-B induced COX-2 (Tunstall et al, 2006) and MMP-2 expression and inhibits cell migration (Su et al, 2006). COX-2 has been found to be overexpressed in several human cancers (Chan et al, 1999;Chun et al, 2003;Csiki et al, 2005;Heeren et al, 2005;Mascaux et al, 2005;Peng et al, 2005;Samoha and Arber, 2005;Tkacz et al, 2005;Wadhwa et al, 2005;Yasuda et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Curcumin down regulates smokeless tobacco-induced NF-κB activation and COX-2 expression in human oral premalignant and cancer cells

et al. 2006

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Cyclooxygenase-2 Expression Increases with the Stage and Grade in Transitional Cell Carcinoma of the Urinary Bladder

Abstract: The degree of COX-2 expression is significantly increased with advancing grade and T stage of disease (p < 0.05).

Cited by 44 publications

References 31 publications

Reciprocal correlation between the expression of cyclooxygenase-2 and E-cadherin in human bladder transitional cell carcinomas

Reciprocal correlation between the expression of cyclooxygenase-2 and E-cadherin in human bladder transitional cell carcinomas

Differences in Expression of Uroplakin III, Cytokeratin 7, and Cyclooxygenase-2 in Canine Proliferative Urothelial Lesions of the Urinary Bladder

Curcumin down regulates smokeless tobacco-induced NF-κB activation and COX-2 expression in human oral premalignant and cancer cells

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