Cyclooxygenase-2 Expression in Urinary Bladder Transitional Cell Carcinoma and its Association with Clinicopathological Characteristics

Tabriz, Hedieh Moradi; Olfati, Golrokh; Ahmadi, Seyed Ali; Yusefnia, Sudabeh

doi:10.7314/apjcp.2013.14.8.4539

Cited by 14 publications

(11 citation statements)

References 28 publications

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“…COX-2 levels have been found to be high in various tumors (Liu et al, 2006;Gomes et al, 2013;Peng et al, 2013;Tabriz et al, 2013). This protein has an important role in carcinogenesis by promoting increased cell proliferation, reducing apoptosis and acting in angiogenesis (Takedo, 1998).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Phospholipase A₂Group IIA in Esophageal Squamous Cell Carcinoma and Association with Cyclooxygenase-2 Expression

Zhai¹,

Dong²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 15 (21), 9417-9421 IntroductionEsophageal cancer is a highly aggressive malignant disease with a 5-year survival rate of 10% to 15% (Ferri et al., 2012). The incidence of esophageal cancer varies greatly between populations and across geographic regions. The Taihangshan Mountain area, including Linxian in Henan Province, a rural area of China, has the highest incidence of esophageal cancer, especially squamous cell carcinoma (ESCC), in the world (>100 per 100, 000 population) (Zhang et al., 2011;Lin et al., 2013).Inflammation has been recognized as a contributing factor for the pathogenesis of ESCC. There are results showing that two key inflammatory enzymes, Phospholipase A 2 Group IIA (PLA2G2A) and cyclooxygenase-2 (COX-2) are involved in the pathways of arachidonic acid (AA) biosynthesis, and both play an important role in inflammation and angiogenesis as well as cancer (Murakami et al., 2011). Recently, we reported that PLA2G2A and COX-2 gene single nucleotide polymorphisms (SNPs) may modify the risk of ESCC

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Section: Discussionmentioning

confidence: 99%

Overexpression of Phospholipase A₂Group IIA in Esophageal Squamous Cell Carcinoma and Association with Cyclooxygenase-2 Expression

Zhai¹,

Dong²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Tabriz et al (2013) found a statistically significant relationship between COX-2 expression and different grades of urinary bladder TCC, and found that high-grade tumors showed a higher expression of COX-2 versus other grades. Tabriz et al (2013) found a statistically significant relationship between COX-2 expression and different grades of urinary bladder TCC, and found that high-grade tumors showed a higher expression of COX-2 versus other grades.…”

Section: Discussionmentioning

confidence: 93%

Immunohistochemical expression of cyclooxygenase-2 and CD44 (stem cell marker) in urinary bladder cancer

Hamid¹,

Raouf²

2014

Egyptian Journal of Pathology

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Background Bladder cancer is the most common urologic cancer and schistosomiasis is responsible for the high incidence of bladder cancer in Egypt. Inflammation may activate stem cells by prostaglandin E2 production mediated by cyclooxygenase-2 (COX-2) expression. Recently, evidence has been accumulating to show that stem cells are involved in inflammation-related carcinogenesis. The cell adhesion molecule CD44 standard (CD44s) has been identified as a cell surface marker associated with cancer stem cell in several types of tumors including urinary bladder cancer.Aim This study aimed to evaluate the expression of COX-2 and stem cell marker CD44s in schistosomal and nonschistosomal bladder tissues obtained from cystitis and cancer patients.Materials and methods Fifty formalin-fixed and paraffinembedded bladder tissues were obtained from four patients with chronic cystitis, 28 patients with transitional cell carcinoma (TCC) (18 schistosomal and 10 nonschistosomal), and 18 patients with squamous cell carcinoma (SCC) (12 schistosomal and six nonschistosomal), and stained by COX-2 and CD44 immunohistochemical markers. ResultsThere was a significant relation between COX-2 expression and schistosomal infection in TCC (P = 0.003), whereas there was no significant difference in the immunoexpression of COX-2 in different grades of TCC. There was no significant relation between CD44 immunostaining and schistosomal infection in TCC and SCC, whereas there was significant difference in the immunoexpression of CD44 and different grades of TCC (P = 0.041). There was a significant difference in the expression of both COX-2 and CD44 among different grades of SCC (P = 0.003 and 0.007, respectively).Conclusion There was a strong association between urinary bladder schistosomiasis infection and increased risks of developing TCC and SCC of the urinary bladder. We found that there was a significant positive association between CD44 (stem cell marker) and COX-2 expression in schistosomal-associated lesions of the urinary bladder (P < 0.0001).

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“…TGF-β1, transforming growth factor β1; EMT, epithelial-mesenchymal transition; COX-2, prostaglandin-endoperoxide synthase 2; ZEB1, zinc finger E-box binding homeobox 1; si, small interfering RNA. expressed in bladder TCC and positively associated with tumor grade (38). However, whether COX-2 plays a role in TGF-β1-induced EMT is unclear.…”

Section: Discussionmentioning

confidence: 99%

Silibinin attenuates TGF‑β1‑induced migration and invasion via EMT suppression and is associated with COX‑2 downregulation in bladder transitional cell carcinoma

Sun

Jia

et al. 2018

Oncol Rep

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Transforming growth factor (TGF)-β1 is highly expressed in bladder transitional cell carcinoma (TCC) and is positively associated with tumor grade. TGF-β1 signaling promotes cell metastasis by inducing epithelial-mesenchymal transition (EMT), however, the underlying mechanisms are not fully understood. Our previous study demonstrated the anti-metastatic effects of silibinin, a natural flavonoid derived from milk thistle, against TCC. The present study investigated the effects of silibinin on TGF-β1-induced EMT in TCC, focusing on the role of prostaglandin-endoperoxide synthase 2 (COX-2). Cell migration was determined by a wound healing assay and Transwell migration assay, and cell invasion was investigated using a Transwell invasion assay. Cell morphology was observed using an inverted microscope. Cell viability was evaluated by an MTT and cell counting assays. EMT markers were detected by reverse transcription-quantitative polymerase chain reaction and western blotting. Specific small interfering RNA was used to knockdown COX-2 gene expression. TGF-β1 promoted cell migration and invasion, induced EMT and upregulated the expression of COX-2. COX-2 knockdown attenuated TGF-β1-induced EMT, indicating that COX-2 upregulation was essential for TGF-β1-induced EMT. Silibinin attenuated TGF-β1-induced migration and invasion by inhibiting EMT, and was associated with COX-2 downregulation. TGF-β1-induced COX-2 upregulation, which was inhibited by silibinin. In addition, TGF-β1-induced EMT was further inhibited when silibinin treatment was combined with COX-2-knockdown. The results suggested that silibinin may be a potential future treatment for metastatic TCC.

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Cyclooxygenase-2 Expression in Urinary Bladder Transitional Cell Carcinoma and its Association with Clinicopathological Characteristics

Cited by 14 publications

References 28 publications

Overexpression of Phospholipase A₂Group IIA in Esophageal Squamous Cell Carcinoma and Association with Cyclooxygenase-2 Expression

Overexpression of Phospholipase A₂Group IIA in Esophageal Squamous Cell Carcinoma and Association with Cyclooxygenase-2 Expression

Immunohistochemical expression of cyclooxygenase-2 and CD44 (stem cell marker) in urinary bladder cancer

Silibinin attenuates TGF‑β1‑induced migration and invasion via EMT suppression and is associated with COX‑2 downregulation in bladder transitional cell carcinoma

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Cyclooxygenase-2 Expression in Urinary Bladder Transitional Cell Carcinoma and its Association with Clinicopathological Characteristics

Cited by 14 publications

References 28 publications

Overexpression of Phospholipase A2Group IIA in Esophageal Squamous Cell Carcinoma and Association with Cyclooxygenase-2 Expression

Overexpression of Phospholipase A2Group IIA in Esophageal Squamous Cell Carcinoma and Association with Cyclooxygenase-2 Expression

Immunohistochemical expression of cyclooxygenase-2 and CD44 (stem cell marker) in urinary bladder cancer

Silibinin attenuates TGF‑β1‑induced migration and invasion via EMT suppression and is associated with COX‑2 downregulation in bladder transitional cell carcinoma

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Overexpression of Phospholipase A₂Group IIA in Esophageal Squamous Cell Carcinoma and Association with Cyclooxygenase-2 Expression

Overexpression of Phospholipase A₂Group IIA in Esophageal Squamous Cell Carcinoma and Association with Cyclooxygenase-2 Expression