Cycloheximide

Lawana, V.; Korrapati, M.C.; Mehendale, H.M.

doi:10.1016/b978-0-12-386454-3.00298-0

Cited by 8 publications

(5 citation statements)

References 8 publications

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“…An antibiotic (ampicillin 3.15 µL/ml) and an antimycotic (cycloheximide 0.2 g/L) were added as well as the DMSO (dimethyl sulfoxide) organic solvent used to dissolve the mutagenic compounds. Cycloheximide was not able to cause gene mutations or unscheduled DNA repair [31], and such evidence was confirmed by our tests (Figure S1). A first run of the test was then performed without exposure, which had a positive outcome (no toxic effect on the strain can be observed) for TA98, TA100, and YG1024, while toxic effects were observed for YG1021 (approximately 60% of spontaneous revertants) in the presence of cycloheximide.…”

Section: Resultssupporting

confidence: 81%

“…A first run of the test was then performed without exposure, which had a positive outcome (no toxic effect on the strain can be observed) for TA98, TA100, and YG1024, while toxic effects were observed for YG1021 (approximately 60% of spontaneous revertants) in the presence of cycloheximide. Such widely used eukaryotic protein synthesis inhibitors sometimes also inhibit bacteria [ 31 ]. Therefore, the sampling campaign began involving the Salmonella typhimurium TA98, TA100, and YG1024 strains.…”

Section: Resultsmentioning

confidence: 99%

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Direct Impact of the Air on Mutant Cells for Mutagenicity Assessments in Urban Environments

Caredda,

Franchitti,

Gilli

et al. 2023

Microorganisms

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Background: Urban air pollution is recognized as a critical problem for public health and is classified as a carcinogen for humans. A great number of studies have focused on the monitoring of urban air mutagenicity. One of the best-known and applied methods for assessing mutagenicity is the Ames test, a bacterial reverse mutation test. The classic protocol for assessing air mutagenicity involves the concentration of particulate matter (PM) on filters and subsequent extraction using organic solvents. This work aimed to develop a method for the evaluation of air mutagenicity directly impacted by air on microbial plates already containing an Ames’ microbial sensor. Methods: A specific six-month sampling campaign was carried out in Turin in a period with high air pollution. Samples were tested for mutagenicity on Salmonella typhimurium strains TA98, TA100, and YG1024 with the traditional method and with the new direct method. Results: The new protocol is able to evaluate the mutagenicity of the sampled air and obtain repeatable results. The final sensitivity is similar to the traditional method (≈10 net revertants/m3); however, the mutagenic response is due to the complete air pollution mixture, including volatile and semivolatile pollutants avoiding the concentration of filters and the following laborious extraction procedures. Conclusions. Despite some critical issues in contamination control, the method is easier, faster, and less expensive than traditional methods.

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Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Direct Impact of the Air on Mutant Cells for Mutagenicity Assessments in Urban Environments

Caredda,

Franchitti,

Gilli

et al. 2023

Microorganisms

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“…Other members of this family show potent cell migration inhibition and antiviral activity [ 4 – 6 ], which continues to capture the attention from researchers in synthetic and biosynthetic chemistry, medicinal chemistry, and pharmacology. However, cycloheximide ( 5 ) has held back the clinical and agricultural applications due to its reproductive toxicity [ 7 ]. Identifying new analogues that offer similar activity without the toxic side-effects could provide a viable lead of therapeutic drugs or agricultural pesticides.…”

Section: Introductionmentioning

confidence: 99%

Cycloheximide congeners produced by Streptomyces sp. SC0581 and photoinduced interconversion between (E)- and (Z)-2,3-dehydroanhydrocycloheximides

Li¹,

Wu²,

Xue³

et al. 2017

Beilstein J. Org. Chem.

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Three new cycloheximide congeners, 2,3-dehydro-α-epi-isocycloheximide (1), (E)- and (Z)-2,3-dehydroanhydrocycloheximides (2 and 3), together with three known compounds, anhydroisoheximide (4), cycloheximide (5), and isocycloheximide (6), were obtained from the cultures of Streptomyces sp. SC0581. Their structures were elucidated by extensive spectroscopic analysis in combination with theoretical conformational analysis and ECD computations. The photoinduced interconversion between 2 and 3 was observed and verified and the possible reaction path and mechanism were proposed by theoretical computations. The antifungal and cytotoxic activities of 1–6 were evaluated and suggested that 2,3-dehydrogenation results in the loss of the activities and supported that the OH-α is important to the activities of cycloheximide congeners.

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“…Additionally, PUR is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase (metallopeptidase). PUR is active against Gram-positive bacteria, less active against acid-fast bacilli, and more weakly active against Gram-negative microorganisms [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

Attachment of Proteolytic Enzyme Inhibitors to Vascular Prosthesis—An Analysis of Binding and Antimicrobial Properties

Mordzińska-Rak,

Szałapata,

Wydrych

et al. 2024

Molecules

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Prosthetic infections are associated with high morbidity, mortality, and relapse rates, making them still a serious problem for implantology. Staphylococcus aureus is one of the most common bacterial pathogens causing prosthetic infections. In response to the increasing rate of bacterial resistance to commonly used antibiotics, this work proposes a method for combating pathogenic microorganisms by modifying the surfaces of synthetic polymeric biomaterials using proteolytic enzyme inhibitors (serine protease inhibitors—4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride and puromycin). While using techniques based on the immobilization of biologically active molecules, it is important to monitor the changes occurring on the surface of the modified biomaterial, where spectroscopic techniques (e.g., FTIR) are ideal. ATR-FTIR measurements demonstrated that the immobilization of both inhibitors caused large structural changes on the surface of the tested vascular prostheses (polyester or polytetrafluoroethylene) and showed that they were covalently bonded to the surfaces of the biomaterials. Next, the bactericidal and antibiofilm activities of the tested serine protease inhibitors were determined using the CLSM microscopic technique with fluorescent staining. During LIVE/DEAD analyses, a significant decrease in the formation of Staphylococcus aureus biofilm after exposure to selected concentrations of native inhibitors (0.02–0.06 mg/mL for puromycin and 0.2–1 mg/mL for 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride) was demonstrated.

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Cycloheximide

Cited by 8 publications

References 8 publications

Direct Impact of the Air on Mutant Cells for Mutagenicity Assessments in Urban Environments

Direct Impact of the Air on Mutant Cells for Mutagenicity Assessments in Urban Environments

Cycloheximide congeners produced by Streptomyces sp. SC0581 and photoinduced interconversion between (E)- and (Z)-2,3-dehydroanhydrocycloheximides

Attachment of Proteolytic Enzyme Inhibitors to Vascular Prosthesis—An Analysis of Binding and Antimicrobial Properties

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