Cyclohexane extract of walnut leaves improves indices of oxidative stress, total homocysteine and lipids profiles in streptozotocin‐induced diabetic rats

Abstract

This study aimed to evaluate the effect of two doses of cyclohexane extract of walnut leaves on total homocysteine, lipids profiles, and indices of oxidative stress including superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and malondialdehyde (MDA) in diabetic rats. Diabetes was induced by a single intraperitoneal (IP) injection of streptozotocin (50 mg/kg BW). Twenty‐eight male Sprague Dawley rats were randomly divided into four groups, group I: control (received sesame oil as vehicle), group II: diabetic control (received sesame oil), group III and IV: diabetic rats treated by 150 and 250 mg/kg body weight (BW) per day extract of walnut leaves, respectively. All groups were treated for 28 days via oral gavage. Fasting blood glucose (FBG) level and body weight measured before injection, 3 days after injection, and on days 0, 7, 14, 21, and 28 of treatment. At the end the 28th day of the experiment, blood samples collected via heart puncture and the sera were used for estimation of the above‐mentioned parameters. The results showed a decrease in FBS, TC, TG, LDL‐c, VLDL‐c, homocysteine, and MDA level and increase in the level of HDL‐c in diabetics treated by walnut leave extracts in a dose‐dependent manner after 28 days. The activity of antioxidant enzymes significantly increased in treated groups compared with diabetic control. It can be concluded that cyclohexane extract of walnut leaves has an overall beneficial effect on body weight, fasting blood glucose, lipids profile, antioxidant enzyme activities, and homocysteine.

<p>Cyclophilin A (CyPA) as a Novel Biomarker for Early Detection of Diabetic Nephropathy in an Animal Model</p>

“…Concerning weight, our results showed that there was a weight loss in the study group after injection rather than before injection, and also in the control group. These results are similar to that of Cheng et al, 56 Mestry et al, 45 Jelodar et al, 55 and Jain and Saha 50 who found that STZ-induced diabetes is associated with a significant reduction in the body weight due to hyperglycemia, hypoinsulinemia, increased muscle wasting and loss of tissue proteins.…”

“…The same thing happened in the study group before and after injection of STZ in accordance with El-Baz et al, 54 who found that animals injected with STZ exhibited a significant elevation in glucose blood levels after 30 days by 2.7-folds, when compared to normal animals. Moreover, Jelodar et al 55 evaluated blood glucose level and found hyperglycemia in different study groups.…”

Impact of Nutritional Intervention on Serum Level of Interferon Gamma and Insulin Resistance in Obese Women: Considerations during the COVID-19 Crisis

“…Improving body weight and its related metabolic syndrome can be obtained by healthy balanced diet, physical activity along with supplements by dietary functional foods that have a role in improving obesity and other diseases [33], where foods, plants, and herbal medicine contain a huge amount of natural phytochemicals and other bioactive components that have a positive role on many diseases without any side effects like medications [33]. In this context, the aim of this study was assessing the efficiency of two dietary supplements on obesity and obesity related metabolic disorders and interferon gamma production as an important immune activator cytokine in immune function.…”

Prevention of Diabetic Complications by Walnut Leaf Extract via Changing Aldose Reductase Activity: An Experiment in Diabetic Rat Tissue

“…This reduction was more obvious in the lens of diabetic treatment 1 compared to diabetic treatments 2 and 3. It can be suggested that the action of antioxidants present in the walnut leaves [36] improves the activity of AR in the diabetic groups. The results of this study are consistent with the results obtained by Yoshida et al and Goodarzi et al [55,58], and it can be concluded that the antioxidants and quercetin in the walnut leaf may be effective in reducing the activity of the aldose reductase.…”

“…The animals were randomly divided into seven equal groups (n = 8), according to the following plan, and treated for 30 days by gavage. The selected doses were according to our previous studies [20,36].…”