Cyclodextrins as catalysts for the removal of cholesterol from macrophage foam cells.

Atger, V.; Moya, Margarita de la Llera; Stoudt, Genevieve; Rodrigueza, Wendi V.; Phillips, Michael C.; Rothblat, George H.

doi:10.1172/jci119223

Cited by 167 publications

(151 citation statements)

References 38 publications

(37 reference statements)

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“…Movement of cholesterol molecules from the PM to acceptor lipoprotein involves an aqueous diffusion mechanism in which the cholesterol desorbs from the membrane and is incorporated into the acceptor after traversing the intervening aqueous phase Mendez and Uint 1996;Atger et al 1997). Net movement of cholesterol is determined by the processes of in ux and ef ux, and in large part on LCAT-catalyzed sterol esteri cation.…”

Section: Apolipoprotein A-i and Efflux Studies With Cultured Cellsmentioning

confidence: 99%

“…Small HDL particles serve as intermediate shuttles ferrying cholesterol from PM to larger HDL particles that function as reservoirs ("sinks") where cholesterol is converted to CE (Johnson et al 1991;Fielding and Fielding 1995a;Fournier et al 1996;Rodrigueza et al 1997). Certain apoLPs in their free form interact with cholesterol-loaded macrophages to generate HDL-like particles, and as a result reduce intracellularly accumulated CEs (Hara and Yokoyama 1991).…”

Section: Importance Of Size Of Apoa-i Particlesmentioning

confidence: 99%

“…Large unilamellar vesicles (LUVs, diameter¸1 l m) made from POPC also were noted to strip HDL particles off unesteri ed cellular cholesterol (Rodrigueza et al 1997). Apart from removing cholesterol from HDL particles, the LUVs donate PL to the particles allowing them to ef ux more cholesterol from Fu5AH cells (Rodrigueza et al 1997).…”

Section: Use Of Liposome Acceptors In Cell Culturementioning

confidence: 99%

“…Apart from removing cholesterol from HDL particles, the LUVs donate PL to the particles allowing them to ef ux more cholesterol from Fu5AH cells (Rodrigueza et al 1997).…”

Section: Use Of Liposome Acceptors In Cell Culturementioning

confidence: 99%

“…At high CD concentrations (10-100 mM), CD per se can trap cholesterol and serve as a sink (Kilsdonk et al 1995;Yancey et al 1996). Atger et al (1997) noted that although CDs added to serum enhanced the exchange of cholesterol between cells and serum, only the combination of CD and LUVs shifted the equilibrium and produced net clearance.…”

Section: Cyclodextrins and Cholesterol Effluxmentioning

confidence: 99%

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Apolipoprotein A-I, Phospholipid Vesicles, and Cyclodextrins as Potential Anti-Atherosclerotic Drugs: Delivery, Pharmacokinetics, and Efficacy

Dass

2000

Drug Delivery

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Section: Apolipoprotein A-i and Efflux Studies With Cultured Cellsmentioning

confidence: 99%

Section: Importance Of Size Of Apoa-i Particlesmentioning

confidence: 99%

Section: Use Of Liposome Acceptors In Cell Culturementioning

confidence: 99%

“…Apart from removing cholesterol from HDL particles, the LUVs donate PL to the particles allowing them to ef ux more cholesterol from Fu5AH cells (Rodrigueza et al 1997).…”

Section: Use Of Liposome Acceptors In Cell Culturementioning

confidence: 99%

Section: Cyclodextrins and Cholesterol Effluxmentioning

confidence: 99%

See 3 more Smart Citations

Apolipoprotein A-I, Phospholipid Vesicles, and Cyclodextrins as Potential Anti-Atherosclerotic Drugs: Delivery, Pharmacokinetics, and Efficacy

Dass

2000

Drug Delivery

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Multifunctional Block Copolymers for Simultaneous Solubilization of Poorly Water‐Soluble Cholesterol and Hydroxyapatite Crystals

Keckeis

Drabinová

Ruiz‐Agudo

et al. 2019

Adv Funct Materials

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The solubilization of targeted compounds represents key criteria in the sophisticated field of medical chemistry but also in technical applications like scale removal. Especially, the simultaneous dissolution of two chemically different compounds remains challenging. Herein, macromolecular solubilizers are introduced for the simultaneous dissolution and encapsulation of poorly water-soluble cholesterol and hydroxyapatite. The peptide-based, amphiphilic block copolymers possess physicochemically disparate segments combined in one polymer chain as binding sites for hydrophobic as well as ionic materials. Small polymer libraries are synthesized and screened for structure-property relationships. Complementary analytical techniques suggest polymeric self-assembly into spherical adaptive nanoparticles with the fundamental ability to passively absorb significant amounts of hydrophobic cholesterol up to 33 wt%. Furthermore, the additional incorporation of acidic domains enables the simultaneous dissolution of hydrophobic compounds and mineral phases such as hydroxyapatite. Ultimately, those nontoxic block copolymers can be used to solubilize and absorb other lipophilic and ionic compounds such as Sudan III dye and calcium ions. Such multifunctional nanomaterials have a wide range of direct application for simultaneous dissolution or delivery of hydrophobic molecules and cations resp. minerals for instance in the field of atherosclerosis.

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Modulation of CD36‐mediated lipid accumulation and senescence by vitamin E analogs in monocytes and macrophages

et al. 2022

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The CD36/FAT scavenger receptor/fatty acids transporter regulates cellular lipid accumulation important for inflammation, atherosclerosis, lipotoxicity, and initiation of cellular senescence. Here we compared the regulatory effects of the vitamin E analogs alpha‐tocopherol (αT), alpha‐tocopheryl phosphate (αTP), and αTP/βCD (a nanocarrier complex between αTP and β‐cyclodextrin [βCD]) and investigated their regulatory effects on lipid accumulation, phagocytosis, and senescence in THP‐1 monocytes and macrophages. Both, αTP and αTP/βCD inhibited CD36 surface exposition stronger than αT leading to more pronounced CD36‐mediated events such as inhibition of DiI‐labeled oxLDL uptake, phagocytosis of fluorescent Staphylococcus aureus bioparticles, and cell proliferation. When compared to βCD, the complex of αTP/βCD extracted cholesterol from cellular membranes with higher efficiency and was associated with the delivery of αTP to the cells. Interestingly, both, αTP and more so αTP/βCD inhibited lysosomal senescence‐associated beta‐galactosidase (SA‐β‐gal) activity and increased lysosomal pH, suggesting CD36‐mediated uptake into the endo‐lysosomal phagocytic compartment. Accordingly, the observed pH increase was more pronounced with αTP/βCD in macrophages whereas no significant increase occurred with αT, alpha‐tocopheryl acetate (αTA) or βCD. In contrast to αT and αTA, the αTP molecule is di‐anionic at neutral pH, but upon moving into the acidic endo‐lysosomal compartment becomes protonated and thus is acting as a base. Moreover, it is expected to be retained in lysosomes since it still carries one negative charge, similar to lysosomotropic drugs. Thus, treatment with αTP or αTP/βCD and/or inhibition of conversion of αTP to αT as it occurs in aged cells may counteract CD36‐mediated overlapping inflammatory, senescent, and atherosclerotic events.

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Cyclodextrins as catalysts for the removal of cholesterol from macrophage foam cells.

Cited by 167 publications

References 38 publications

Apolipoprotein A-I, Phospholipid Vesicles, and Cyclodextrins as Potential Anti-Atherosclerotic Drugs: Delivery, Pharmacokinetics, and Efficacy

Apolipoprotein A-I, Phospholipid Vesicles, and Cyclodextrins as Potential Anti-Atherosclerotic Drugs: Delivery, Pharmacokinetics, and Efficacy

Multifunctional Block Copolymers for Simultaneous Solubilization of Poorly Water‐Soluble Cholesterol and Hydroxyapatite Crystals

Modulation of CD36‐mediated lipid accumulation and senescence by vitamin E analogs in monocytes and macrophages

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