Cyclodextrin and its derivatives enhance protein crystallization by grafted on crystallization plates

Wang, Qianjin; Zhao, Gang; Zhang, Chenyan

doi:10.1016/j.jcrysgro.2020.125591

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…Therefore, modifying the plate surface can provide tailored structures that promote crystal growth, improve crystal quality, and enhance the crystallization success rate. For instance, Wang et al [ 10 ] found that grafting cyclodextrin and its derivatives onto crystallization plates improved protein crystallization, and this strategy is both labor-efficient and beneficial for automated crystallization screening. However, the effectiveness of this strategy depends on the grafting density of cyclodextrin or its derivatives, making it difficult to determine the optimal thickness for new proteins.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening

et al. 2023

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Modifying crystallization plates can significantly impact the success rate and quality of protein crystal growth, making it a helpful strategy in protein crystallography. However, appropriate methods for preparing nano-sized particles with a high specific surface area and strategies for applying these nanoparticles to form suitable coatings on crystallization plate surfaces still need to be clarified. Here, we utilized both an ultrasonic crusher and a high-pressure homogenizer to create a nano metal–organic framework (MOF), specifically HKUST-1, and introduced a solvent evaporation method for producing MOF coatings on 96-well crystallization plates to induce protein crystal growth. The morphology of MOF coatings on the resin surface of the plate well was characterized using optical and scanning electron microscopy. Compared to the control group, crystallization screening experiments on nine proteins confirmed the effectiveness of plates with MOF coatings. Applying MOF coatings to crystallization plates is an easy-to-use, time-efficient, and potent tool for initiating crystallization experiments.

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Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening

et al. 2023

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“…With the advancement of the biopharmaceutical market, protein drugs have been developed rapidly and have attracted extensive attention from the academic community. − Compared with traditional small molecule drugs, protein drugs have many advantages, such as good biocompatibility and high efficacy, showing great potential in the treatment of cancer, metabolic diseases, and autoimmune diseases . However, due to the extremely complex structure of proteins and the relatively slower crystallization kinetics, the preparation of protein crystals and protein–drug complex crystals is still a tough task. − Besides, many external factors, such as the protein concentration, temperature, pH, precipitants, buffers, and other special environmental factors, make the crystallization process of protein even more complicated .…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Continuous Crystallization of Lysozyme through Ultrasound

Mao

Zhao

et al. 2021

Org. Process Res. Dev.

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Protein drugs have attracted extensive attention from the academic community. However, crystallization of proteins is difficult and the yield of current main technologies is too low, which limits their large-scale industrial production. In this work, enhancement of continuous crystallization of lysozyme under an ultrasonic field was explored. A plug-flow tubular crystallizer, which was separated into a nucleation zone and a growth zone, was applied. The effect of ultrasound on steady-state time, crystal habit, particle size, particle size distribution, and the process yield was evaluated based on univariate analysis experiments. It was found that ultrasound can shorten the time for continuous crystallization to reach a steady state, reduce the degree of aggregation, and promote nucleation. Furthermore, the separation of the growth zone from the nucleation zone in the ultrasonic-assisted continuous crystallization can further increase the size of the lysozyme crystals, which would help in increasing the yield.

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Nitrogen-doped carbon embedded with Ag nanoparticles for bidirectionally-promoted polysulfide redox electrochemistry

Zhang

Cai

Liu

et al. 2022

Chemical Engineering Journal

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Cyclodextrin and its derivatives enhance protein crystallization by grafted on crystallization plates

Cited by 3 publications

References 36 publications

Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening

Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening

Enhancement of Continuous Crystallization of Lysozyme through Ultrasound

Nitrogen-doped carbon embedded with Ag nanoparticles for bidirectionally-promoted polysulfide redox electrochemistry

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