Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair

Li, Chongjie; Ma, Li; Mou, Shanli; Wang, Yibin; Zheng, Zhou; Liu, Fangming; Qi, Xiaoqing; An, Meiling; Chen, Hao; Miao, Jun

doi:10.1016/j.mrfmmm.2014.07.010

Cited by 18 publications

(11 citation statements)

References 24 publications

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“…To clone a recombinant expression vector, the full-length photolyase gene was inserted into pEASY-Blunt E1 (Amp + ) (TransGen Biotech). After being transformed into Transetta (DE3) Chemically Competent Cell (TransGen Biotech) and sequenced (Sangon), correct clones were stored with glycerol at − 80 °C (Li et al 2015). The expression vector encodes for a protein with a C-terminal His-tag.…”

Section: Recombinant Protein Expression and Purificationmentioning

confidence: 99%

“…We measured enzyme activity as reported (Li et al 2015(Li et al , 2010 with some adjustments. The repair activity of CPD photolyase was measured as following method: the CPD substrates were created by irradiation DNA oligomers 5′-TTT TTT TT TTT TTT TT-3′ (Sangon) for 4 h with UV (Beijing Normal University, Beijing, wavelength 290-320 nm, 8 W) on ice.…”

Section: In Vitro Assays For Photolyase Activitymentioning

confidence: 99%

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Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H

Miao

et al. 2021

3 Biotech

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Two gene of class II photolyases, PiPhr1 (1833 bp) and PiPhr2 (1809 bp), from the Antarctic diatom Phaeodactylum tricornutum ICE-H were cloned, the recombinant proteins expressed and purified. The molecular weight of the recombinant photolyases were determined to be 68 kDa with a pI of 9.04 and 68.82 with a pI of 7.31, respectively. Activity studies showed that both the recombinant enzymes were involved in the repair DNA damaged by UV light, that is they were most likely photolyases involved in photorepair of DNA. Further confirmation of this function was demonstrated by the increased expression of PiPhr1 and PiPhr2 after exposure to UV radiation, blue light and dark conditions by qRT-PCR. In summary, PiPhr1 and PiPhr2 were up regulated by UVB irradiation and blue light at 0.5 h and 3 h. Longtime (3 h) exposure to dark also increased the expression of PiPhr1 and PiPhr2. In vitro photoreactivation assays showed that PiPhr1 and PiPhr2 could repair CPDs utilizing blue light. This is the first time CPD Class II photolyase has been reported from Antarctic diatom. These results will add to the knowledge of the diatom CPF family and assist in understanding the functional role of these genes in Antarctic diatoms.

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Section: Recombinant Protein Expression and Purificationmentioning

confidence: 99%

Section: In Vitro Assays For Photolyase Activitymentioning

confidence: 99%

Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H

Miao

et al. 2021

3 Biotech

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“…Photolyases have been identified in the Antarctic marine bacteria Pseudomonas , Janthinobacterium , Flavobacterium , Hymenobacter and Sphingomonas [ 3 , 355 ], in different species of Antarctic diatoms [ 96 ], in the microalga Chlamydomonas sp. ICE-Lis [ 356 ], in the bipolar terrestrial moss Sanionia uncinata (Hedw.) Loeske [ 357 ], in the notothenioid fish, Notothenia coriiceps and Chaenocephalus aceratus , and in the krill Euphausia superba [ 103 ].…”

Section: Uv Photoprotection In Marine Organisms: Antarctic and Nonmentioning

confidence: 99%

“… Taxonomy Compound Activity Reference Phylum: Proteobacteria, Bacteroidetes Class: Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Flavobacteriia Pseudomonas, Janthinobacterium, Flavobacterium, Hymenobacter, Sphingomonas Photolyase DNA repair [ 3 , 355 ] Phylum: Ciliophora Class: Spirotrichea Euplotes focardii Heat shock protein, Hsp70 Chaperone in the UV stress [ 382 ] Phylum: Chlorophyta Class: Chlorophyceae Chlamydomonas sp. ICE-L Photolyase DNA repair [ 356 ] Heat shock protein, Hsp70 Chaperone in the UV stress [ 381 ] Phylum: Ochrophyta Class: Bacillariophyceae Chaetoceros neglectus, Corethron cryophilum, Coscinodiscus ocutus-iridis, Odontella weissflogii, Porosira pseudodenticulata, Thalassiosira subtilis, Eucampia antarctica, Chaetoceros convolutus, Chaetocerois socialis, Thalassiosira australis, Licmophora decora, Nitzchia kerguelensis Photolyase/NER DNA repair [ 96 ] Phylum: Echinodermata …”

Section: Table A1mentioning

confidence: 99%

UV-Protective Compounds in Marine Organisms from the Southern Ocean

et al. 2018

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Solar radiation represents a key abiotic factor in the evolution of life in the oceans. In general, marine, biota—particularly in euphotic and dysphotic zones—depends directly or indirectly on light, but ultraviolet radiation (UV-R) can damage vital molecular machineries. UV-R induces the formation of reactive oxygen species (ROS) and impairs intracellular structures and enzymatic reactions. It can also affect organismal physiologies and eventually alter trophic chains at the ecosystem level. In Antarctica, physical drivers, such as sunlight, sea-ice, seasonality and low temperature are particularly influencing as compared to other regions. The springtime ozone depletion over the Southern Ocean makes organisms be more vulnerable to UV-R. Nonetheless, Antarctic species seem to possess analogous UV photoprotection and repair mechanisms as those found in organisms from other latitudes. The lack of data on species-specific responses towards increased UV-B still limits the understanding about the ecological impact and the tolerance levels related to ozone depletion in this region. The photobiology of Antarctic biota is largely unknown, in spite of representing a highly promising reservoir in the discovery of novel cosmeceutical products. This review compiles the most relevant information on photoprotection and UV-repair processes described in organisms from the Southern Ocean, in the context of this unique marine polar environment.

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“…Remarkably, both photolyases are absent in placental mammals (including humans and mice) [ 14 , 15 ]. CPD photolyases have been found in bacteria, fungi, plants, invertebrates, vertebrates, and marsupial mammals [ 14 , 16 , 17 , 18 , 19 , 20 ]. Previous findings indicated humans and mice do not have the genes encoding CPD photolyases [ 15 ]; moreover, as a macromolecule, CPD photolyases do not penetrate the skin directly.…”

Section: Introductionmentioning

confidence: 99%

Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

Liu

et al. 2022

IJMS

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UVB radiation is known to trigger the block of DNA replication and transcription by forming cyclobutane pyrimidine dimer (CPD), which results in severe skin damage. CPD photolyase, a kind of DNA repair enzyme, can efficiently repair CPDs that are absent in humans and mice. Although exogenous CPD photolyases have beneficial effects on skin diseases, the mechanisms of CPD photolyases on the skin remain unknown. Here, this study prepared CPD photolyase nanoliposomes (CPDNL) from Antarctic Chlamydomonas sp. ICE-L, which thrives in harsh, high-UVB conditions, and evaluated their protective mechanisms against UVB-induced damage in mice. CPDNL were optimized using response surface methodology, characterized by a mean particle size of 105.5 nm, with an encapsulation efficiency of 63.3%. Topical application of CPDNL prevented UVB-induced erythema, epidermal thickness, and wrinkles in mice. CPDNL mitigated UVB-induced DNA damage by significantly decreasing the CPD concentration. CPDNL exhibited antioxidant properties as they reduced the production of reactive oxygen species (ROS) and malondialdehyde. Through activation of the NF-κB pathway, CPDNL reduced the expression of pro-inflammatory cytokines including IL-6, TNF-α, and COX-2. Furthermore, CPDNL suppressed the MAPK signaling activation by downregulating the mRNA and protein expression of ERK, JNK, and p38 as well as AP-1. The MMP-1 and MMP-2 expressions were also remarkably decreased, which inhibited the collagen degradation. Therefore, we concluded that CPDNL exerted DNA repair, antioxidant, anti-inflammation, and anti-wrinkle properties as well as collagen protection via regulation of the NF-κB/MAPK/MMP signaling pathways in UVB-induced mice, demonstrating that Antarctic CPD photolyases have the potential for skincare products against UVB and photoaging.

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Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair

Cited by 18 publications

References 24 publications

Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H

Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H

UV-Protective Compounds in Marine Organisms from the Southern Ocean

Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

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