Cycloartane triterpene glycosides from the hairy root cultures of Astragalus membranaceus

Hirotani, Masao; Zhou, Yu; Furuya, Hekai Rut aTsutomu

doi:10.1016/s0031-9422(00)90420-5

Cited by 70 publications

(43 citation statements)

References 9 publications

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“…It also possesses hepatoprotective, antioxidative, antiviral, antihypertensive, and immuno-stimulant properties (Rios and Waterman, 1997). A. membranaceus roots have been reported to contain triterpene saponins, phenolic compounds, and polysaccharides (Hirotami et al, 1994;Subarnas et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Variation of Phenolic Compounds Contents in Cultivated Astragalus membranaceus

Jun¹,

Kim²,

Lim³

et al. 2012

Korean Journal of Medicinal Crop Science

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: This study was conducted to determine the contents of phenolic compounds and astragalosides in Astragalus membranaceus. Between the phenolic compound groups, flavonoids compounds (51.13%) had higher average concentrations than the phenolic acids groups. Among the 30 phenolic compounds, quercetin (353.11 ㎍ g -1 ) provided the highest concentrations. According to different cultivation year, 3-year-cultivated Astragalus (2612.57 ㎍ g -1 ) showed the highest concentrations of phenolic compounds. According to different harvest days, 6/5 harvesting Astragalus (2615.80 ㎍ g -1 ) showed the highest concentrations of phenolic acids. Comparison between the top and subterranean parts of harvested Astragalus plants cultivated for 2 years, 3 years, and 5 years showed big difference of total phenolic compounds in concentrations. Further, the top part had higher amounts of the total phenolic compounds than the subterranean part among all Astragalus. This tendency was similar to those of the top root and lateral root. The concentration of the phenolic compounds in the top root was higher than that of the lateral root.

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Section: Introductionmentioning

confidence: 99%

Variation of Phenolic Compounds Contents in Cultivated Astragalus membranaceus

Jun¹,

Kim²,

Lim³

et al. 2012

Korean Journal of Medicinal Crop Science

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show abstract

“…It possesses many biological functions, including hepatoprotective, antioxidative, antiviral, antihypertensive and immunostimulant properties and can strengthen the superficial resistance, discharge of pus and the growth of new tissues (Xie et al, 1994;Rios and Waterman, 1997). The roots have been reported to contain triterpene saponins, isoflavonoids and polysaccharides (Hirotani et al, 1994;Shimizu et al, 1991;Tomoda et al, 1992;Subarnas et al, 1991;Lin et al, 2000). Calycosin-7-O-β-D-glucopyranoside (3′-hydroxy-4′-methoxyisoflavone-7-O-β-D-glucopyranoside; Fig.…”

Section: Introductionmentioning

confidence: 99%

Determination of calycosin‐7‐O‐β‐d‐glucopyranoside in rat plasma and urine by HPLC

Guan

et al. 2007

Biomedical Chromatography

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A reversed-phase high-performance liquid chromatographic (HPLC) assay for calycosin-7-O-beta-D-glucopyranoside in rat plasma and urine with solid-phase extraction (SPE) was developed. Rutin was employed as an internal standard. The mobile phase consisted of acetonitrile-water (16:84, v/v) at a flow rate of 1.0 mL/min. Detection was set at 280 nm. The limit of quantitation of calycosin-7-O-beta-D-glucopyranoside was 0.2 microg/mL in both plasma and urine. The standard curve was linear from 0.2 to 10.0 microg/mL in plasma, and 0.2 to 5.0 microg/mL in urine. Both intra- and inter-day precision of the calycosin-7-O-beta-d-glucopyranoside were determined and their RSD did not exceed 10%. The method was successfully applied to the analysis of samples obtained from a basic pharmacokinetic study, in which calycosin-7-O-beta-d-glucopyranoside was administered orally to rats.

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“…membranaceus roots have been reported to contain triterpene saponins, phenolic compounds, and polysaccharides (Hirotami et al, 1994;Subarnas et al, 1991). A. membranaceus is an important ingredient in many traditional Chinese formulas.…”

Section: Introductionmentioning

confidence: 99%

Variation of Astragalosides Contents in Cultivated Astragalus membranaceus

Kim¹,

Jun²,

Lim³

et al. 2012

Korean Journal of Medicinal Crop Science

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: This study was conducted to determine the contents of astragalosides in Astragalus membranaceus. The total astragaloside content (1701 ㎍ g -1 ) of 2 year-cultivated astragalus was the highest among the variously cultivated astragalus plants. Upon an increase in cultivation time, the average value of the total astragaloside content decreased (from 1650 ㎍ g -1 to 645 ㎍ g -1 ). Especially, the content of astragaloside decreased rapidly. Comparison between the top and subterranean parts of harvested astragalus plants showed that as the cultivation time increased, the astragaloside content of the top part decreased. On the other hand, the subterranean part demonstrated the opposite pattern. The astragaloside content of the top root increased compared to that in the lateral root (from 1.9 ㎍ g -1 to 33.4 ㎍ g -1 ). Further, the content of astragaloside increased in the top root as the cultivation time increased.

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Cycloartane triterpene glycosides from the hairy root cultures of Astragalus membranaceus

Cited by 70 publications

References 9 publications

Variation of Phenolic Compounds Contents in Cultivated Astragalus membranaceus

Variation of Phenolic Compounds Contents in Cultivated Astragalus membranaceus

Determination of calycosin‐7‐O‐β‐d‐glucopyranoside in rat plasma and urine by HPLC

Variation of Astragalosides Contents in Cultivated Astragalus membranaceus

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