1995
DOI: 10.1073/pnas.92.12.5386
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Cyclins as markers of tumor proliferation: immunocytochemical studies in breast cancer.

Abstract: We have developed methods to use anticyclin A, B, and E antibodies as reagents to specifically detect proliferating cells in specific phases of the cell cycle in formalin-fixed, paraffin-embedded sections of tissues and cells. Staining of 48 archival cases of breast cancer showed that these antibodies estimate the tumor proliferation fraction and therefore are potentially useful for the prediction of prognosis. A subset of cancers had a high frequency of tumor cells expressing cyclins A and E, out of proportio… Show more

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Cited by 151 publications
(110 citation statements)
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“…Along with other researchers (Dutta et al, 1995;Paterlini et al, 1995), we found that the expression of cyclin A is closely correlated to the proportion of S-phase cells as measured by flow cytometry (data not shown). Univariate analysis indicates that cyclin A may also be a good prognostic indicator for the survival of patients with squamous cell lung carcinomas, but this was no longer seen in the multivariate analysis.…”
Section: Discussionsupporting
confidence: 86%
“…Along with other researchers (Dutta et al, 1995;Paterlini et al, 1995), we found that the expression of cyclin A is closely correlated to the proportion of S-phase cells as measured by flow cytometry (data not shown). Univariate analysis indicates that cyclin A may also be a good prognostic indicator for the survival of patients with squamous cell lung carcinomas, but this was no longer seen in the multivariate analysis.…”
Section: Discussionsupporting
confidence: 86%
“…In addition, cyclin E expression was significantly associated with a high proliferation fraction, which had previously been demonstrated in the immunohistochemical study by Dutta et al (1995). Dutta et al identified a small fraction of tumours that overexpressed cyclin E relative to proliferation.…”
Section: Evaluation Carcinomassupporting
confidence: 59%
“…The following were used: mouse monoclonal antibody against cyclin E (HE12) generated against recombinant human cyclin E protein (Santa Cruz), as used by Dutta et al (1995). (In immunoblotting it recognizes the major human cyclin E-encoded proteins as three bands including a doublet at around 50 kDa and a single band at 42 kDa.)…”
Section: Antibodiesmentioning
confidence: 99%
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