CyclinD1, p53, E‐cadherin, and VEGF discordant expression in paired regional metastatic lymph nodes of esophageal squamous cell carcinoma: A tissue array analysis

Luo, Kongjia; Hu, Yi; Wen, Jing; Fu, Jianhua

doi:10.1002/jso.21921

Cited by 20 publications

(16 citation statements)

References 38 publications

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“…Among these, the E-cadherin expression in 134 paired PTs and MLNs was reported previously [13]. The tissue microarray cores from 8 pairs of PTs and MLNs and 13 PTs without paired MLNs were excluded from our analysis because they were dropped during the immunohistochemistry (IHC) procedures and so could not be used.…”

Section: Resultsmentioning

confidence: 99%

“…A mouse monoclonal anti-E-cadherin antibody (Maxim, 1:100 dilution) was used for E-cadherin staining with an Elivision plus IHC kit (Maxim, Fuzhou, China), as described previously [13]. N-cadherin and vimentin were stained using a SPlink Detection kit (ZSGB-BIO, Beijing, China) with rabbit monoclonal anti-N-cadherin (Epitomics, Burlingame, CA, 1:500 dilution) and anti-vimentin (Epitomics, 1:250 dilution) antibodies.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the combined results from IHC staining of epithelial (E-cadherin) and mesenchymal (N-cadherin and vimentin) markers, each ESCC PT or MLN specimen was classified into one of the following four categories, as described previously [13, 14] wild type (positive for E-cadherin and negative for both mesenchymal markers), null type (negative for both E-cadherin and mesenchymal markers), hybrid type (positive for both E-cadherin and any mesenchymal marker), and complete type (negative for E-cadherin and positive for any mesenchymal marker) [14, 15]. …”

Section: Methodsmentioning

confidence: 99%

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The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients

et al. 2016

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Epithelial-mesenchymal transition (EMT) plays a key role in tumor metastasis, but the significance of EMT phenotype to the prognosis of esophageal squamous cell carcinoma (ESCC) patients remains unclear. We used immunohistochemistry to examine the expression of the EMT-related proteins E-cadherin, N-cadherin and vimentin in samples of T3N1-3M0 ESCC from 155 primary tumors (PTs) with paired metastatic lymph nodes (MLNs) and 58 PTs without paired MLNs. Based on the expression pattern of the EMT markers, PTs and MLNs were classified as EMT wild, hybrid, null or complete type. The hybrid (42.7%) and complete (39.4%) types predominated among PTs, whereas the wild (34.2%) and hybrid (52.9%) types predominated among MLNs, and EMT phenotypes differed between the paired PTs and MLNs (P < 0.001). Univariate analysis revealed that, for PTs, the EMT phenotype was associated with N-stage (P = 0.039) but not patient survival, and that patients with complete or hybrid type MLNs had better overall survival (OS, P = 0.001) and disease-free survival (DFS, P = 0.005) than patients with null and wild type MLNs, especially those with N1-stage disease (P = 0.017 for OS, and P = 0.017 for DFS, respectively). Multivariate analysis revealed that wild and null type MLNs as well as older age and N2-3 stage were independent predictors of OS and DFS (P < 0.05). Thus MLNs exhibit EMT phenotypes that are distinct from those of their PT and may serve as a novel independent prognostic indicator in ESCC.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients

et al. 2016

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“…We also found that SRF gene silencing strongly inhibits the cellular invasion that accompanies the upregulation of E-cadherin. Consequently, inhibiting the expression of E-cadherin blocks its activity in cell-cell adhesion, cancer invasion and metastasis (18). …”

Section: Discussionmentioning

confidence: 99%

Serum response factor is overexpressed in esophageal squamous cell carcinoma and promotes Eca-109 cell proliferation and invasion

Zhao

et al. 2013

Oncology Letters

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Recent studies indicate that serum response factor (SRF) is highly expressed in tumors such as hepatocellular, thyroid, esophageal and lung carcinoma. However, the expression and roles of SRF in esophageal squamous cell carcinoma (ESCC) are unclear. In this study, immunohistochemistry was used to compare the expression of SRF in ESCC cases (n=73) and normal controls (n=30). The RNA interference (RNAi) technique was used to knock down the expression of SRF in Eca-109 cells. Cell proliferation, cell cycle stage and invasion were measured with cell counting kit (CCK)-8, flow cytometry and Transwell assays, respectively. Western blotting was used to measure SRF, E-cadherin, β-catenin and cyclin D1 expression in Eca-109 cells treated with siRNA. The study demonstrated that human ESCC has increased expression of SRF. In addition, blocking SRF expression inhibited tumor proliferation and invasion. In conclusion, SRF has the potential to be a new marker for ESCC diagnosis and therapy.

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“…Several studies investigating ESCC pathophysiology have focused on changes in protein expression, which can contribute to the discovery of new biomarkers and help to develop novel strategies for the early diagnosis and individualized treatment of ESCC [1]. It was previously reported that p53 [2]–[4], Cyclin D1 [5]–[7], E-cadherin [8], K-ras [9], and VEGF [10]–[13] have altered expression in ESCC, suggesting that abnormalities in the expression of oncogenes and tumor suppressor genes contributes to esophageal carcinogenesis and malignant development. These abnormally expressed proteins may therefore serve as potential biomarkers for ESCC.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of OLC1 Promotes Tumorigenesis of Human Esophageal Squamous Cell Carcinoma

et al. 2014

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PurposeOLC1 was recently identified to be a potential oncogene. However, the role of OLC1 in human esophageal cell carcinoma (ESCC) is unknown. The aim of this study was therefore to evaluate the expression of OLC1 in human ESCC from normal, premalignant, and malignant lesions, and to clarify the mechanisms by which OLC1 contributes to the progression of ESCC. Experimental DesignTwo hundred and fourteen paired ESCC specimens, and an independent set from 28 ESCC patients, were used to analyze the correlation between OLC1 expression and the pathological characteristics of tumors using immunohistochemistry. Stable OLC1-overexpressing and OLC1-interfering esophageal cancer cells were established and a series of experimental methods were used to investigate the biological functions and mechanisms of action of OLC1.ResultsWe showed that OLC1 was overexpressed in 145 of 214 (67.8%) of human ESCC specimens, compared with in only 59 of 214 (27.57%) paired adjacent normal tissues (P<0.001). OLC1 overexpression occurred at a rate of 35% (10/28) at the stage of mild/moderate dysplasia, but was significantly upregulated to 66% (22/33) at the stages of severe dysplasia and in situ carcinoma, while 71% positive staining (22/28) was observed in invasive carcinoma tissues compared with normal tissues (P<0.05). We also provided evidence that OLC1 abnormalities significantly altered the cell proliferation and apoptosis induced by cytotoxic agents. OLC1 overexpression suppressed apoptosis, and was associated with attenuated caspase-3 activation and increased Bcl-2 stability.ConclusionOur study provides strong evidence suggesting OLC1 abnormalities may contribute to the development of human ESCC and have some important clinical significance.

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CyclinD1, p53, E‐cadherin, and VEGF discordant expression in paired regional metastatic lymph nodes of esophageal squamous cell carcinoma: A tissue array analysis

Cited by 20 publications

References 38 publications

The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients

The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients

Serum response factor is overexpressed in esophageal squamous cell carcinoma and promotes Eca-109 cell proliferation and invasion

Overexpression of OLC1 Promotes Tumorigenesis of Human Esophageal Squamous Cell Carcinoma

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