Cyclin-dependent kinase 2-associated protein 1 suppresses growth and tumorigenesis of lung cancer

Sun, Mei; Jiang, Rui; Wang, Guan; Zhang, Chao; Li, Jindong; Jin, Chengyan; Zhang, Xingyi

doi:10.3892/ijo.2013.1813

Cited by 12 publications

(11 citation statements)

References 24 publications

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“…Recently, several studies have reported negative or decreased DOC‐1 expression in gastric cancer tissue, and this was highly correlated with more advanced tumor stage and invasion [], risk of lymph node metastases, and decreased survival in patients with OSCC []. Similar reports were observed from many cancer types including prostate cancer [], esophageal carcinoma [], breast cancer [], colorectal cancer [], and lung cancer [].…”

Section: Introductionsupporting

confidence: 83%

“…In the present study, we reported the differential mRNA expression of the tumor suppressor deleted in oral cancer‐1 (DOC‐1) gene, which has not been reported previously in relation to the precancerous conditions of OSF, OLP, and LKP, and differential oral habits. Clinically, a decrease in DOC‐1 expression in OSCC and other forms of cancers has been found to correlate with its potential role in malignancy [–], whereas in vivo studies have shown that the overexpression of DOC‐1 in mouse and hamster model of OC, and HCPC‐1 cells significantly induced antitumor response, growth suppression, and an increase in apoptosis and antiproliferative effect [,]. In the present study, a loss of DOC‐1 mRNA expression was observed in 61% of patients with OSCC and a reduced DOC‐1 mRNA expression in 39% of patients.…”

Section: Discussionmentioning

confidence: 45%

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Association of oral tumor suppressor gene deleted in oral cancer‐1 (DOC‐1) in progression of oral precancer to cancer

Katarkar

Patel

Mukherjee

et al. 2014

Oral Science International

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a b s t r a c tIntroduction: Deleted in oral cancer-1 (DOC-1), a highly conserved tumor suppressor gene, encodes a specific cyclin-dependent kinase 2-associated protein 1 (p12 DOC-1 ). The protein DOC-1 arrests the cells in the G 1 phase of the cell cycle and regulates DNA replication in the S phase of the cell cycle. It is known that DOC-1 is downregulated in head and neck cancer. Hence, the aim of the study was to analyze the messenger RNA (mRNA) expressions of DOC-1 in patients with oral lichen planus (OLP), leukoplakia (LPK), oral submucous fibrosis (OSF), and oral squamous cell carcinoma (OSCC) in comparison to normal control subjects, and these were correlated with differential oral habits and duration of exposure. Materials and methods: Semiquantitative one-step reverse transcriptase polymerase chain reaction (RT-PCR) was used to assess the potential role of DOC-1 in differential oral conditions. Results: The expression of DOC-1 at the transcriptional level was found to be consistently reduced (39.13%) or non-detectable (60.87%) in OSCC cases; LPK cases also showed reduced (26.19%) or non-detectable (59.52%) DOC-1 expression, and overexpression of DOC-1 was observed in patients with OSF (42.3%) and OLP (35.29%) as compared to the normal control groups. A marked reduction in DOC-1 expression was observed in patients who smoke bidi and chew tobacco compared to patients who smoke cigarettes and chew pan. An overall reduced expression of DOC-1 with an increase in the duration of exposure was observed.Conclusions: The overexpression of DOC-1 in oral premalignant disease groups with reduced or loss of expression in OSCC groups suggests its association in the progression of oral carcinogenesis, suggesting DOC-1 to be an important prognostic indicator for oral cancer.

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Section: Introductionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 45%

Association of oral tumor suppressor gene deleted in oral cancer‐1 (DOC‐1) in progression of oral precancer to cancer

Katarkar

Patel

Mukherjee

et al. 2014

Oral Science International

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“…A link between altered CDK1 expression and PE has not been previously reported. Vannucci et al [16] described up-regulation of CDK2-associated protein 1 (CDK2AP1), a growth suppressor that down-regulates CDK2 [46]. Since CDK2 has an effect on the cell cycle that is broadly similar to CDK1, it seems counter-intuitive that a CDK2 suppressor protein is up-regulated in PE.…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of mechanisms involved in cell transport and maintenance of mucosal integrity in pigs infected with Lawsonia intracellularis

Smith

Wilson

Ettinger

et al. 2014

Vet Res

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Lawsonia intracellularis is an obligate intracellular bacterium, responsible for the disease complex known as proliferative enteropathy (PE). L. intracellularis is associated with intestinal crypt epithelial cell proliferation but the mechanisms responsible are yet to be defined. Microarray analysis was used to investigate the host-pathogen interaction in experimentally infected pigs to identify pathways that may be involved. Ileal samples originating from twenty-eight weaner pigs experimentally challenged with a pure culture of L. intracellularis (strain LR189/5/83) were subjected to microarray analysis. Microarray transcriptional signatures were validated using immunohistochemistry and quantitative real time PCR of selected genes at various time points post challenge. At peak of infection (14 days post challenge) 86% of altered transcripts were down regulated, particularly those involved in maintenance of mucosal integrity and regulation of cell transport. Among the up-regulated transcripts, CD163 and CDK1 were novel findings and considered to be important, due to their respective roles in innate immunity and cellular proliferation. Overall, targeted cellular mechanisms included those that are important in epithelial restitution, migration and protection; maintenance of stable inter-epithelial cell relationships; cell transport of nutrients and electrolytes; innate immunity; and cell cycle.

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“…Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1), also named as DOC-1 (deleted in oral cancer-1), is a growth suppressor originally isolated from normal hamster oral keratinocytes [ 20 ]. There is evidence to suggest that CDK2AP1, as an inhibitor of CDK2 and hence G1/S transition, is expected to involve cell cycle progression [ 21 ]. In addition, recent studies showed that CDK2AP1 mediates the growth suppressing signal from TGF-β [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

miR-205 promotes proliferation and invasion of laryngeal squamous cell carcinoma by suppressing CDK2AP1 expression

Zhong

Xiong

2015

Biol Res

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BackgroundThe aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. miR-205 was reported to be upregulated in laryngeal squamous cell carcinoma (LSCC) tissues, however, the mechanisms by which miR-205 functions as a regulator of LSCC are largely unknown.ResultsIn this study, Real-time qPCR and Western blot assay showed that expression of miR-205 was upregulated and expression of cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was downregulated in LSCC tissues. The expression levels of miR-205 were negatively related to those of CDK2AP1 in LSCC tissues and cell lines. Moreover, we found that miR-205 was the upstream regulator of CDK2AP1 and could suppress the CDK2AP1 expression in LSCC cells. 3-(4,5-dimethylthiazal-2-yl)-2,5-diphenyl-tetrazolium bromide assays and transwell invasion assay were performed to test the proliferation and invasion of LSCC cells. Gelatin zymography was used to detect the activity of MMP2 and MMP9. CDK2AP1, c-Myc and CyclinD1 expression in cells was assessed with Western blotting. We found that miR-205 was the upstream regulator of CDK2AP1 and could suppress the expression of CDK2AP1 in LSCC cells. In addition, miR-205 significantly induced cell proliferation and invasion by suppressing CDK2AP1 expression. Consistent with miR-205 inhibitors, overexpressed CDK2AP1 suppressed the activity of MMP2 and MMP9 and c-Myc and CyclinD1 expression in LSCC cells.ConclusionThese findings help us to better elucidate the molecular mechanisms of LSCC progression and provide a new theoretical basis to further investigate miR-205 as a potential biomarker and a promising approach for LSCC treatment.

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Cyclin-dependent kinase 2-associated protein 1 suppresses growth and tumorigenesis of lung cancer

Cited by 12 publications

References 24 publications

Association of oral tumor suppressor gene deleted in oral cancer‐1 (DOC‐1) in progression of oral precancer to cancer

Association of oral tumor suppressor gene deleted in oral cancer‐1 (DOC‐1) in progression of oral precancer to cancer

Down-regulation of mechanisms involved in cell transport and maintenance of mucosal integrity in pigs infected with Lawsonia intracellularis

miR-205 promotes proliferation and invasion of laryngeal squamous cell carcinoma by suppressing CDK2AP1 expression

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