Cyclin D1 gene amplification in esophageal carcinosarcoma shown by differential polymerase chain reaction

Suzuki, Hiroaki; Moriya, Junji; Nakahata, Atsuko; Fujioka, Yasunori; Inoue, Kazuaki; Nagashima, Kazuo

doi:10.1016/s0046-8177(98)90273-8

Cited by 16 publications

(6 citation statements)

References 13 publications

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“…This similar p53 immunoreactivity in the two cell types strongly suggests both the neoplastic nature not only of the squamous cells, but also of the spindle cells, and the common clonal origin of the two cell types. These results confirm those obtained in single case reports 11 , 12 , 15 , 16 and are similar to those obtained for carcinosarcomas in other locations 17 –19 . However, p53 protein over‐expression is only an indirect indicator of p53 gene mutation, with many causes of false‐positive (non‐mutational stabilization of the protein) and false‐negative (truncating mutation of the gene) results.…”

Section: Discussionsupporting

confidence: 87%

“…A few genetic studies have been published with conflicting results regarding the type of alteration present in the two tumour components. Some studies favour a common origin for the squamous and the spindle cells by demonstrating the same pattern of genetic alteration; 11 in contrast, other studies have shown distinct features 1 , 11 , 12 . Two studies of the DNA content in SCSC of the oesophagus have been performed by flow cytometry 7 and cytophotometry 6 .…”

Section: Discussionmentioning

confidence: 99%

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Spindle cell squamous carcinoma of the oesophagus: an analysis of 17 cases, with new immunohistochemical evidence for a clonal origin

et al. 2001

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Spindle cell squamous carcinoma of the oesophagus: an analysis of 17 cases, with new immunohistochemical evidence for a clonal origin

et al. 2001

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“…This method for detecting cyclin D1 gene amplification has been used for analyzing several tumor types (21)(22)(23)(24). When first described (21), the authors coamplified a 152-bp fragment of the cyclin D1 gene with a 112-bp fragment of the dopamine D2 receptor gene.…”

Section: Differential Pcrmentioning

confidence: 99%

Cyclin D1 Protein Expression Predicts Metastatic Behavior in Thyroid Papillary Microcarcinomas But Is Not Associated with Gene Amplification

Khoo

Ezzat

Freeman

et al. 2002

The Journal of Clinical Endocrinology &Amp; Metabolism

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Overexpression of cyclin D1 occurs in several malignancies, often due to gene amplification, and this has been associated with aggressive tumor behavior, a higher incidence of lymph node metastases, and a poorer prognosis. The role of cyclin D1 in the pathogenesis of thyroid malignancy is unknown; however, cyclin D1 expression has been reported to occur in a proportion of well differentiated thyroid carcinomas. Micropapillary carcinomas of the thyroid are common incidental findings that almost always behave in an indolent manner and remain quiescent. However, rare microcarcinomas behave aggressively and metastasize early, giving rise to clinically significant disease. We hypothesized that cyclin D1 might play a role in the aggressive behavior of metastasizing papillary microcarcinomas. We reviewed the histopathology reports of 2,000 patients who underwent thyroid surgery at our institution between 1995-1999 and identified 22 patients who presented with gross regional metastases from a primary papillary microcarcinoma. These patients formed the index cohort for this analysis. As controls, we selected 34 patients with nonmetastasizing microcarcinomas. We studied these tumors for immunoreactivity to cyclin D1 on immunohistochemistry and analyzed 13 tumors that diffusely expressed cyclin D1 for gene amplification by differential PCR. Twenty of the 22 (90.9%) metastasizing papillary microcarcinomas expressed cyclin D1, compared with 3 of the 34 (8.8%) nonmetastasizing papillary microcarcinomas (P < 0.001). However, of the 13 tumors that showed diffuse immunoreactivity for cyclin D1 on immunohistochemistry, none showed amplification of the cyclin D1 gene on differential PCR. We conclude that cyclin D1 is significantly overexpressed in metastasizing papillary microcarcinomas of the thyroid. This is likely due to mechanisms other than gene amplification. Cyclin D1 immunohistochemistry may be a valuable tool in predicting metastatic potential in papillary microcarcinomas.

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“…Studies have shown CCND1 and CTTN are likely to be the candidate oncogenes within this region on the basis that only these two genes have been found to be both amplified and overexpressed in tumors (5). In ESCC, the role of CCND1 has been well elucidated (6)(7)(8)(9)(10)(11). Array CGH exhibited increase of CTTN DNA copy number in esophageal tumors (4,12), but the precise role of CTTN in ESCC tumorigenesis and progression is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Amplification and Overexpression ofCTTN(EMS1) Contribute to the Metastasis of Esophageal Squamous Cell Carcinoma by Promoting Cell Migration and Anoikis Resistance

et al. 2006

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Gain of chromosome 11q13 is a common event in esophageal squamous cell carcinoma (ESCC). The cortactin gene (CTTN, also EMS1), located at 11q13, plays a pivotal role in coupling membrane dynamics to cortical actin assembly. This gene has been implicated in the motility of several types of cells. In the present study, we found that the amplification and overexpression of the CTTN gene was associated with lymph node metastasis in ESCC. Functional analysis by small interfering RNA-mediated silencing of CTTN revealed that in addition to the effect on cell migration, CTTN influenced cell invasiveness by anoikis resistance. In vivo assay showed that inhibition of CTTN expression also decreased tumor growth and lung metastasis of ESCC cells. At the molecular level, we showed for the first time that the protective role of CTTN in anoikis resistance was correlated with the activation of the phosphatidylinositol 3-kinase/Akt pathway. Overall, the data suggest that CTTN is an oncogene in the 11q13 amplicon and exerts functions on tumor metastasis in ESCC.

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Cyclin D1 gene amplification in esophageal carcinosarcoma shown by differential polymerase chain reaction

Cited by 16 publications

References 13 publications

Spindle cell squamous carcinoma of the oesophagus: an analysis of 17 cases, with new immunohistochemical evidence for a clonal origin

Spindle cell squamous carcinoma of the oesophagus: an analysis of 17 cases, with new immunohistochemical evidence for a clonal origin

Cyclin D1 Protein Expression Predicts Metastatic Behavior in Thyroid Papillary Microcarcinomas But Is Not Associated with Gene Amplification

Amplification and Overexpression ofCTTN(EMS1) Contribute to the Metastasis of Esophageal Squamous Cell Carcinoma by Promoting Cell Migration and Anoikis Resistance

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