Cyclin D1 gene amplification in proliferating haemangioma

Mohamed, Amal M.; Elwakil, Tarek F.; Taher, Ibrahim M.; Elbarbary, Mohamed M.; Kayed, Hesham F.; Hussein, Hassan A.; Eid, Ola M.

doi:10.1007/s00441-009-0858-y

Cited by 7 publications

(5 citation statements)

References 26 publications

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“…17) Consistently with anti-proliferative activity by CK in bFGF-treated HUVECs, Compound K inhibited the expression of cyclin D1 in bFGF-treated HUVECs in a concentration dependent manner by Western blotting (Fig. 2C).…”

Section: Ck Inhibits Bfgf-induced Proliferation In Huvecssupporting

confidence: 71%

Compound K Inhibits Basic Fibroblast Growth Factor-Induced Angiogenesis via Regulation of p38 Mitogen Activated Protein Kinaseand AKT in Human Umbilical Vein Endothelial Cells

Jeong

Lee

Jeong

et al. 2010

Biological & Pharmaceutical Bulletin

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Section: Ck Inhibits Bfgf-induced Proliferation In Huvecssupporting

confidence: 71%

Compound K Inhibits Basic Fibroblast Growth Factor-Induced Angiogenesis via Regulation of p38 Mitogen Activated Protein Kinaseand AKT in Human Umbilical Vein Endothelial Cells

Jeong

Lee

Jeong

et al. 2010

Biological & Pharmaceutical Bulletin

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“…23 CyclinD1 gene amplification has been verified to promote abnormal endothelial cell proliferation and angiogenesis in proliferating HAs. 24 Importantly, it has been shown that knockdown of HIF-1a downregulates Bcl-2 expression, but upregulates p53 expression in cancer cells. 25,26 Our present study indicated that OMT decreased the expression of Bcl-2 and CyclinD1, and increased p53 expression, suggesting that OMT might participate in the antitumor effect of HA via HIF-1a-mediated downregulation of Bcl-2 and CyclinD1 and upregulation of p53 expression.…”

Section: Discussionmentioning

confidence: 99%

Oxymatrine suppresses proliferation and induces apoptosis of hemangioma cells through inhibition of HIF-1a signaling

et al. 2015

Int J Immunopathol Pharmacol

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Oxymatrine (OMT), a natural quinolizidine alkaloid, has been known to have anti-inflammation, anti-anaphylaxis, and chemopreventive effects on various cancer cells. To clarify the underlying role and molecular mechanisms of OMT in human hemangioma (HA), in the present study, we examined the expression of hypoxia-inducible factor-1a (HIF-1a) and vascular endothelial growth factor (VEGF) in different phases of human HA. After HA derived endothelial cells (HDEC) were pretreated with different concentrations of OMT, cell proliferation, apoptosis, and cycle distribution were evaluated by MTT assay and flow cytometry analysis, respectively. The effects of OMT on expression of HIF-1a signaling were determined by real-time PCR and western blot assays. Our results showed that, the expression of HIF-1a and VEGF was significantly increased in proliferating phase HA, but decreased in involuting phase HA. Moreover, OMT in vitro inhibited proliferative activities and induced cell apoptosis and cycle arrest in G 0 /G 1 phase in HA cells with decreased expression of HIF-1a, VEGF, Bcl-2, and CyclinD1, and increased expression of p53. Taken together, our findings suggest that, the expression of HIF-1a and VEGF is increased in proliferating phase HA, and OMT suppresses cell proliferation and induces cell apoptosis and cycle arrest in proliferative phase HA through inhibition of the HIF-1a signaling pathway, suggesting OMT may provide a novel therapeutic strategy for the treatment of HA.

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“…Some studies have shown the association between these indexes and HAs. PCNA and Ki-67 are expressed by the majority of endothelial cells in the proliferating phase HAs, but their expression is negligible in the involuting phase (29)(30)(31). Bcl-2 contributes to the low apoptosis effect seen in HAs (32).…”

Section: Discussionmentioning

confidence: 99%

Knockdown of IGF2R suppresses proliferation and induces apoptosis in hemangioma cells in vitro and in vivo

et al. 2014

International Journal of Oncology

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Abstract. Insulin-like growth factor-II (IGF-II)/IGF2R signaling plays a pivotal role in cell growth, migration and differentiation in many malignancies. An individual with high IGF-II expression levels has a high risk of developing cancer, but IGF2R is often considered to be a tumor suppressor. To date, little has been reported about the role of IGF-II/IGF2R signaling in hemangiomas (HAs). Thus, uncovering the mechanisms of IGF-II/IGF2R signaling is very important to understanding the development of HAs. In the present study, the expression of IGF-II and IGF2R was investigated in 27 cases of HAs of different phases by immunohistochemistry. Through lentivirus-mediated IGF2R siRNA (Lv-siIGF2R) in HA-derived endothelial cells (HDECs), we observed the effects of IGF2R knockdown on the biological behavior of HA cells. We found that the expression of IGF-II and IGF2R was significantly increased in proliferating phase HAs, but decreased in involuting phase HAs. Furthermore, knockdown of IGF2R in vitro significantly diminished the proliferative activity and induced apoptosis and cycle arrest with decreased expression of PCNA, Ki-67, Bcl-2, Cyclin D1 and E and increased the expression of Bax in the proliferative phase HAs (HDEC and CRL-2586 EOMA cells). In addition, the tumor volumes in a subcutaneous HDEC nude mouse model treated with Lv-siIGF2R were significantly smaller than those of the control group. Taken together, our findings indicate that the expression of IGF-II and IGF2R is increased in proliferating phase HAs, and knockdown of IGF2R suppresses proliferation and induces apoptosis in HA cells in vitro and in vivo, suggesting that IGF2R may represent a novel therapeutic target for the treatment of human HAs.

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Cyclin D1 gene amplification in proliferating haemangioma

Cited by 7 publications

References 26 publications

Compound K Inhibits Basic Fibroblast Growth Factor-Induced Angiogenesis via Regulation of p38 Mitogen Activated Protein Kinaseand AKT in Human Umbilical Vein Endothelial Cells

Compound K Inhibits Basic Fibroblast Growth Factor-Induced Angiogenesis via Regulation of p38 Mitogen Activated Protein Kinaseand AKT in Human Umbilical Vein Endothelial Cells

Oxymatrine suppresses proliferation and induces apoptosis of hemangioma cells through inhibition of HIF-1a signaling

Knockdown of IGF2R suppresses proliferation and induces apoptosis in hemangioma cells in vitro and in vivo

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