Cyclin A is a reliable proliferation marker in endometrial cancer cell lines

Horie, Kayo; H, Yamamoto; Karube, Kouhei; Takebayashi, Kai; Yoshino, Hironori; Yoshioka, Haruhiko; Watanabe, Jun

doi:10.3892/ol.2019.10135

Cited by 11 publications

(12 citation statements)

References 25 publications

(30 reference statements)

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“…Cyclin A depletion caused the reduction of DNA synthesis activity [ 31 ]. In addition, recent evidence suggests that cyclin A could be regarded as a proliferation marker [ 32 ]. Given our data showed that Fubp1 is a transcription activator of genes encoding cyclin A, we examined the role of Fubp1 in cell proliferation.…”

Section: Resultsmentioning

confidence: 99%

Multiple Functions of Fubp1 in Cell Cycle Progression and Cell Survival

Kang

Kim

et al. 2020

Cells

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The discovery of novel and critical genes implicated in malignant development is a topic of high interest in cancer research. Intriguingly, a group of genes named “double-agent” genes were reported to have both oncogenic and tumor-suppressive functions. To date, less than 100 “double-agent” genes have been documented. Fubp1 is a master transcriptional regulator of a subset of genes by interacting with a far upstream element (FUSE). Mounting evidence has collectively demonstrated both the oncogenic and tumor suppressive roles of Fubp1 and the debate regarding its roles in tumorigenesis has been around for several years. Therefore, the detailed molecular mechanisms of Fubp1 need to be determined in each context. In the present study, we showed that the Fubp1 protein level was enriched in the S phase and we identified that Fubp1 deficiency altered cell cycle progression, especially in the S phase, by downregulating the mRNA expression levels of Ccna genes encoding cyclin A. Although this Fubp1-cyclin A axis appears to exist in several types of tumors, Fubp1 showed heterogeneous expression patterns among various cancer tissues, suggesting it exhibits multiple and complicated functions in cancer development. In addition, we showed that Fubp1 deficiency confers survival advantages to cells against metabolic stress and anti-cancer drugs, suggesting that Fubp1 may play both positive and negative roles in malignant development.

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Section: Resultsmentioning

confidence: 99%

Multiple Functions of Fubp1 in Cell Cycle Progression and Cell Survival

Kang

Kim

et al. 2020

Cells

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“…USP7 might also contribute to oncogenesis by deubiquitinating and stabilizing Ki-67 ( Zhang et al, 2016 ). As with Cyclin A2, Ki-67 is highly expressed in malignant tissues and promotes cell division ( Sales Gil and Vagnarelli, 2018 ; Horie et al, 2019 ). This interaction may be particular disease-relevant in non-small cell lung cancer cells, where a strong positive correlation between the two proteins has been observed ( Zhang et al, 2016 ).…”

Section: Usp7: Links With Cancermentioning

confidence: 99%

USP7 Is a Master Regulator of Genome Stability

Valles

Bezsonova

Woodgate

et al. 2020

Front. Cell Dev. Biol.

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Genetic alterations, including DNA mutations and chromosomal abnormalities, are primary drivers of tumor formation and cancer progression. These alterations can endow cells with a selective growth advantage, enabling cancers to evade cell death, proliferation limits, and immune checkpoints, to metastasize throughout the body. Genetic alterations occur due to failures of the genome stability pathways. In many cancers, the rate of alteration is further accelerated by the deregulation of these processes. The deubiquitinating enzyme ubiquitin specific protease 7 (USP7) has recently emerged as a key regulator of ubiquitination in the genome stability pathways. USP7 is also deregulated in many cancer types, where deviances in USP7 protein levels are correlated with cancer progression. In this work, we review the increasingly evident role of USP7 in maintaining genome stability, the links between USP7 deregulation and cancer progression, as well as the rationale of targeting USP7 in cancer therapy.

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“…D9 caused significant decreases in cyclin D1, indicating the growth inhibitory effects on cyclin D1 of D9 (Figure C–E). Besides, the cyclin B1 and CDK1 complex plays crucial roles in regulating the transition from the G2 to the M phase and the expression of cyclin A, which promotes both cell cycle G1/S and G2/M transitions . We discovered that D9 increased the protein levels of cyclin B1, CDK1 and cyclin A in a concentration‐dependent manner (Figure C–E).…”

Section: Resultsmentioning

confidence: 78%

Discovering Podophyllotoxin Derivatives as Potential Anti‐Tubulin Agents: Design, Synthesis and Biological Evaluation

Han

et al. 2020

ChemistrySelect

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Podophyllotoxin is a potent cytotoxic agent and serves as a useful lead compound for development of anticancer agents. Tubulin is known as an attractive target for drug discovery and cancer therapies. There are few studies on the treatment of breast cancer with podophyllotoxin and its derivatives targeting tubulin. Here, a series of novel aryl 1,3,4‐oxadiazole/1,3,4‐thiadiazole acid podophyllotoxin ester derivatives (D1–D16) were synthesized. Among these compounds, D9 exhibited excellent antiproliferation activity against MCF‐7 cells (IC50=2.46±0.12 μM). Furthermore, D9 caused cell cycle arrest at the G2/M phase and induced cell apoptosis. Confocal microscopy showed that D9 inhibited microtubule polymerization by causing cancer cell growth inhibition. Molecular docking results suggested D9 could bind to the active binding site of tubulin. In a mouse model, compound D9 suppressed malignant growth without causing significant toxicity to normal tissues. Our findings support the utility of D9 as a novel compound for the development of anticancer agent.

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Cyclin A is a reliable proliferation marker in endometrial cancer cell lines

Cited by 11 publications

References 25 publications

Multiple Functions of Fubp1 in Cell Cycle Progression and Cell Survival

Multiple Functions of Fubp1 in Cell Cycle Progression and Cell Survival

USP7 Is a Master Regulator of Genome Stability

Discovering Podophyllotoxin Derivatives as Potential Anti‐Tubulin Agents: Design, Synthesis and Biological Evaluation

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