Cyclical Interactions between Two Outer Doublet Microtubules in Split Flagellar Axonemes

Aoyama, Susumu; Kamiya, Ritsu

doi:10.1529/biophysj.105.067876

Cited by 64 publications

(67 citation statements)

References 44 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There has been no clear identification of the second mode that appears to be necessary. The only examples of oscillation involving a clearly understood OFF mode occur when doublets become visibly separated by transverse forces, and then reanneal to resume active sliding, which again causes bending and doublet separation [Sale, 1986;Aoyama and Kamiya, 2005]. Under normal conditions, the OFF mode does not involve this type of extreme separation.…”

Section: Indications For Two Persistent Modes Of Dynein Operationmentioning

confidence: 99%

Thinking about flagellar oscillation

Brokaw

2008

Cell Motil. Cytoskeleton

136

133

View full text Add to dashboard Cite

Bending of cilia and flagella results from sliding between the microtubular outer doublets, driven by dynein motor enzymes. This review reminds us that many questions remain to be answered before we can understand how dynein-driven sliding causes the oscillatory bending of cilia and flagella. Does oscillation require switching between two distinct, persistent modes of dynein activity? Only one mode, an active forward mode, has been characterized, but an alternative mode, either inactive or reverse, appears to be required. Does switching between modes use information from curvature, sliding direction, or both? Is there a mechanism for reciprocal inhibition? Can a localized capability for oscillatory sliding become self-organized to produce the metachronal phase differences required for bend propagation? Are interactions between adjacent dyneins important for regulation of oscillation and bend propagation? Cell Motil. Cytoskeleton 66: 425-436, 2009. '

show abstract

Section: Indications For Two Persistent Modes Of Dynein Operationmentioning

confidence: 99%

Thinking about flagellar oscillation

Brokaw

2008

Cell Motil. Cytoskeleton

136

133

View full text Add to dashboard Cite

show abstract

“…1 of the previous modeling paper [Brokaw, 2009] and Fig. 2 of the experimental paper [Aoyama and Kamiya, 2005]. Dyneins remaining attached at a separation of 35 nm were less than 1 per cycle; these were programmatically detached.…”

Section: Cyclic Sliding and Splitting Of A Doublet Pairmentioning

confidence: 88%

“…4, which can be compared with Fig. 4 of the experimental paper [Aoyama and Kamiya, 2005]. The transition criterion used is a doublet separation of 40 nm, which is 11 nm beyond the point at which dynein shear force falls to 0 (Fig.…”

Section: Cyclic Sliding and Splitting Of A Doublet Pairmentioning

confidence: 99%

“…Early work described experimental conditions where a portion of an axoneme can transiently separate into two or three bundles of doublets, which then reassociate and continue to generate active sliding [Sale, 1986;Brokaw, 1986a]. Kamiya and Okagaki [1986] and Aoyama and Kamiya [2005] used enzymatic digestion to partially deconstruct Chlamydomonas axonemes, and observed pairs of doublets that remained firmly connected at their basal ends. Shear forces and sliding generated by dyneins on one doublet caused cycles of bending, separation, and reassociation.…”

Section: Introductionmentioning

confidence: 99%

“…The ability of this computer modeling to simulate the experimental results demonstrates that both structural and motor functions of dynein can be explained by conventional ideas about the chemical kinetics of dynein motors. In the two-doublet experimental system [Aoyama and Kamiya, 2005], some structural components were eliminated by the enzymatic digestion used to deconstruct the axoneme. The current simulation programming has also been expanded to mathematically replace some functions of the missing components, so that it is possible to examine the operation of a "geometric clutch" [Lindemann, 1994a] during bend propagation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Computer simulation of flagellar movement X: Doublet pair splitting and bend propagation modeled using stochastic dynein kinetics

Brokaw

2014

Cytoskeleton

View full text Add to dashboard Cite

Experimental observations on cyclic splitting and bending by a flagellar doublet pair are modeled using forces obtained from a model for dynein mechanochemistry, based on ideas introduced by Andrew Huxley and Terrill Hill and extended previously for modeling flagellar movements. The new feature is elastic attachment of dynein to the A doublet, which allows movement perpendicular to the A doublet and provides adhesive force that can strain attached dyneins. This additional strain influences the kinetics of dynein attachment and detachment. Computations using this dynein model demonstrate that very simple and realistic ideas about dynein mechanochemistry are sufficient for explaining the separation and reattachment seen experimentally with flagellar doublet pairs. Additional simulations were performed after adding a "super-adhesion" elasticity. This elastic component is intended to mimic interdoublet connections, normally present in an intact axoneme, that would prevent visible splitting but allow sufficient separation to cause dynein detachment and cessation of shear force generation. This is the situation envisioned by Lindemann's "geometric clutch" hypothesis for control of dynein function in flagella and cilia. The simulations show abrupt disengagement of the "clutch" at one end of a bend, and abrupt reengagement of the "clutch" at the other end of a bend, ensuring that active sliding is only operating where it will cause bend propagation from base to tip. V C 2014

show abstract

A novel mechanism of sperm motility in a viscous environment: Corkscrew‐shaped spermatozoa cruise by spinning

Muto

2009

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

Fertilization of the green tree frog, Rhacophorus arboreus, occurs in the viscous environment of a foam nest, which is laid on vegetation. Their spermatozoa have a characteristic corkscrew-shaped head and a thick tail that extends perpendicularly to its longitudinal axis. However, it is unclear how these corkscrew-shaped spermatozoa move in this highly viscous environment. Here, we found that the spinning of the corkscrew-shaped head, caused by winding and unwinding of the tail, enables the spermatozoa to move through the highly viscous environment of a foam nest, like a corkscrew rotating into a cork. We suggested that dislocations observed in the matrix of satellite microtubules surrounding two axonemes, reflected the planes of sliding of the axonemes, and dyneins on doublets two and six of each axoneme were active during winding and unwinding, respectively. These results provide a novel mechanism for sperm movement that is adapted specifically to a viscous fertilization environment.

show abstract

Cyclical Interactions between Two Outer Doublet Microtubules in Split Flagellar Axonemes

Cited by 64 publications

References 44 publications

Thinking about flagellar oscillation

Thinking about flagellar oscillation

Computer simulation of flagellar movement X: Doublet pair splitting and bend propagation modeled using stochastic dynein kinetics

A novel mechanism of sperm motility in a viscous environment: Corkscrew‐shaped spermatozoa cruise by spinning

Contact Info

Product

Resources

About