Cyclic Stretch of Either PNS or CNS Located Nerves Can Stimulate Neurite Outgrowth

Kampanis, Vasileios; Tolou-Dabbaghian, Bahardokht; Zhou, Luming; Roth, Wolfgang; Puttagunta, Radhika

doi:10.3390/cells10010032

Cited by 7 publications

(5 citation statements)

References 96 publications

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“…A tailored mechanical stretch (with a strain of 0.1-1% for cultured neurons and up to 10% for DRG explants) had positive effects on neurite outgrowth and pain modulation [27,30,36,37]. Higher levels of stretch had negative effects on neurons, such as impaired neurite regeneration and increased neuronal death [27,33,34,[36][37][38].…”

Section: Evidence From the Petri Dishmentioning

confidence: 99%

Neurodynamics: is tension contentious?

Ellis

Carta

Andrade

et al. 2021

Journal of Manual & Manipulative Therapy

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Tensioning techniqueswere the first neurodynamic techniques used therapeutically in the management of people with neuropathies. This article aims to provide a balanced evidenceinformed view on the effects of optimal tensile loading on peripheral nerves and the use of tensioning techniques. Whilst the early use of neurodynamics was centered within a mechanical paradigm, research into the working mechanisms of tensioning techniques revealed neuroimmune, neurophysiological, and neurochemical effects. In-vitro and ex-vivo research confirms that tensile loading is required for mechanical adaptation of healthy and healing neurons and nerves. Moreover, elimination of tensile load can have detrimental effects on the nervous system. Beneficial effects of tensile loading and tensioning techniques, contributing to restored homeostasis at the entrapment site, dorsal root ganglia and spinal cord, include neuronal cell differentiation, neurite outgrowth and orientation, increased endogenous opioid receptors, reduced fibrosis and intraneural scar formation, improved nerve regeneration and remyelination, increased muscle power and locomotion, less mechanical and thermal hyperalgesia and allodynia, and improved conditioned pain modulation. However, animal and cellular models also show that 'excessive' tensile forces have negative effects on the nervous system. Although robust and designed to withstand mechanical load, the nervous system is equally a delicate system. Mechanical loads that can be easily handled by a healthy nervous system, may be sufficient to aggravate clinical symptoms in patients. This paper aims to contribute to a more balanced view regarding the use of neurodynamics and more specifically tensioning techniques.

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Section: Evidence From the Petri Dishmentioning

confidence: 99%

Neurodynamics: is tension contentious?

Ellis

Carta

Andrade

et al. 2021

Journal of Manual & Manipulative Therapy

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“…Moreover, stretch is also found to stimulate neurite growth of mature neurons. Ten percent cyclic stretch of nerve explants at 0.5 Hz enhanced neurite outgrowth of neurons from rat dorsal root ganglia ( Kampanis et al, 2020 ), and 10 pN of stretch could enhance axon growth and branching ( De Vincentiis et al, 2020 ). However, the conclusions of the amplitude of cyclic strain that can induce neurite outgrowth or neural differentiation are different from these studies.…”

Section: Discussionmentioning

confidence: 99%

“…A recent study has revealed that extracellular physical cues could transduce into intracellular force to control the intestinal organoid growth and development through Wnt/β-catenin signaling ( Li et al, 2020 ). Particularly, stretch could stimulate neuron growth ( Loverde and Pfister, 2015 ; Breau and Schneider-Maunoury, 2017 ), axon growth ( De Vincentiis et al, 2020 ), and neurite outgrowth ( Higgins et al, 2013 ; Kampanis et al, 2020 ). Moreover, we have reported that fluid shear stimulation could boost BMSC differentiation into endothelial cells and cardiomyocyte-like cells ( Bai et al, 2010 ; Huang et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Cyclic Strain and Electrical Co-stimulation Improve Neural Differentiation of Marrow-Derived Mesenchymal Stem Cells

Cheng

Huang

Chen

et al. 2021

Front. Cell Dev. Biol.

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The current study investigated the combinatorial effect of cyclic strain and electrical stimulation on neural differentiation potential of rat bone marrow-derived mesenchymal stem cells (BMSCs) under epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2) inductions in vitro. We developed a prototype device which can provide cyclic strain and electrical signal synchronously. Using this system, we demonstrated that cyclic strain and electrical co-stimulation promote the differentiation of BMCSs into neural cells with more branches and longer neurites than strain or electrical stimulation alone. Strain and electrical co-stimulation can also induce a higher expression of neural markers in terms of transcription and protein level. Neurotrophic factors and the intracellular cyclic AMP (cAMP) are also upregulated with co-stimulation. Importantly, the co-stimulation further enhances the calcium influx of neural differentiated BMSCs when responding to acetylcholine and potassium chloride (KCl). Finally, the phosphorylation of extracellular-signal-regulated kinase (ERK) 1 and 2 and protein kinase B (AKT) was elevated under co-stimulation treatment. The present work suggests a synergistic effect of the combination of cyclic strain and electrical stimulation on BMSC neuronal differentiation and provides an alternative approach to physically manipulate stem cell differentiation into mature and functional neural cells in vitro.

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“…It is also interesting to note that there are conflicting reports in terms of injury severity in mild stretch models [ 91 ], with some studies demonstrating beneficial effects of mild nerve stretch [ 92 , 93 ]. In contrast, severe axonal stretching induces critical and permanent deficits, including mitochondrial swelling and cell death [ 94 , 95 , 96 ].…”

Section: Axonal Stretch Injury Modelmentioning

confidence: 99%

A Brief Review of In Vitro Models for Injury and Regeneration in the Peripheral Nervous System

Varier

Raju

Madhusudanan

et al. 2022

IJMS

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Nerve axonal injury and associated cellular mechanisms leading to peripheral nerve damage are important topics of research necessary for reducing disability and enhancing quality of life. Model systems that mimic the biological changes that occur during human nerve injury are crucial for the identification of cellular responses, screening of novel therapeutic molecules, and design of neural regeneration strategies. In addition to in vivo and mathematical models, in vitro axonal injury models provide a simple, robust, and reductionist platform to partially understand nerve injury pathogenesis and regeneration. In recent years, there have been several advances related to in vitro techniques that focus on the utilization of custom-fabricated cell culture chambers, microfluidic chamber systems, and injury techniques such as laser ablation and axonal stretching. These developments seem to reflect a gradual and natural progression towards understanding molecular and signaling events at an individual axon and neuronal-soma level. In this review, we attempt to categorize and discuss various in vitro models of injury relevant to the peripheral nervous system and highlight their strengths, weaknesses, and opportunities. Such models will help to recreate the post-injury microenvironment and aid in the development of therapeutic strategies that can accelerate nerve repair.

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Cyclic Stretch of Either PNS or CNS Located Nerves Can Stimulate Neurite Outgrowth

Cited by 7 publications

References 96 publications

Neurodynamics: is tension contentious?

Neurodynamics: is tension contentious?

Cyclic Strain and Electrical Co-stimulation Improve Neural Differentiation of Marrow-Derived Mesenchymal Stem Cells

A Brief Review of In Vitro Models for Injury and Regeneration in the Peripheral Nervous System

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