Cyclic Phosphatidic Acid Relieves Osteoarthritis Symptoms

Gotoh, Mari; Nagano, Aya; Tsukahara, Ryoko; Murofushi, Hiromu; Morohoshi, Toshiro; Otsuka, Kazuyuki; Murakami‐Murofushi, Kimiko

doi:10.1186/1744-8069-10-52

Cited by 17 publications

(16 citation statements)

References 30 publications

(73 reference statements)

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“…Cell culture was performed as previously described ( 25 ). Human chondrogenic SW1353 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with streptomycin (100 µg/ml) and penicillin (100 U/ml; both Thermo Fisher Scientific, Inc.) in 25 cm 2 culture flasks at 37°C in a humidified atmosphere with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3

et al. 2018

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Methylene blue (MB) is a long-term inhibitor of peripheral nerve axons, thereby alleviating or permanently eliminating pain. However, it remains unknown whether MB is safe and effective method of treating osteoarthritis (OA). MB was injected into the knee joints of rabbits and they were monitored for any histological structural changes. The results revealed no evident changes in the histological structure of the normal knee joint following injection of 1 mg/kg MB at 1, 4, 8 and 24 weeks post-injection. Compared with the vehicle control, MB treatment significantly enhanced the weight distribution and significantly decreased the swelling ratio of the rabbits. Additionally, levels of long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) mRNA were significantly increased following treatment with MB, but the protein expression of P2X purinoceptor 3 (P2X3) was significantly suppressed compared with the vehicle control. The levels of interleukin (IL) 6, tumor necrosis factor (TNF)α, IL-1β and IL-8 were significantly suppressed following MB treatment, indicating that MB protects against OA progression. It was also revealed that MEG3 overexpression significantly suppresses levels of P2X3 protein. ELISA indicated that the MEG3-induced reduction of IL-6, TNFα, IL-1β and IL-8 expression was significantly reversed following P2X3 overexpression. Therefore, the results of the present study demonstrated that MB is an effective method of treating OA-associated pain by upregulating lncRNA MEG3 levels. Additionally, lncRNA MEG3 relieves the OA-associated pain and inflammation in a rabbit model of OA by inhibiting P2X3 expression.

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Section: Methodsmentioning

confidence: 99%

Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3

et al. 2018

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“…Considering the breadth of bioactivities of this class of lipids [ 47 ], their contributions to immune responses may be diverse, particularly since they modulate nuclear function [ 48 , 49 ]. In this regard, pharmacological studies have shown that cPA analogs potently reverse experimental osteoarthritis [ 50 ], block immune-induced demyelination [ 51 ], and inhibit the growth of cancer cells [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses

et al. 2018

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Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during infection have been described, but their utility as a diagnostic marker is still unclear. In this study, we undertook a lipidomics approach to determine if there are any differences in the serum lipid profiles of horses naturally infected with pathogenic Leptospira spp. and horses vaccinated against a commercially available bacterin. Utilizing a high-resolution mass spectrometry serum lipidomics analytical platform, we demonstrate that cyclic phosphatidic acids, diacylglycerols, and hydroperoxide oxidation products of choline plasmalogens are elevated in the serum of naturally infected as well as vaccinated horses. Other lipids of interest were triacylglycerols that were only elevated in the serum of infected horses and sphingomyelins that were increased only in the serum of vaccinated horses. This is the first report looking at the equine serum lipidome during leptospiral infection and vaccination.

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“…For cartilage explant culture, full-thickness cartilage tissue was cut into pieces of 5 mm. Following 48 hrs of culture in Dulbecco's modified Eagle's medium/F-12 containing 10% foetal bovine serum and 50 units/ml of penicillin and streptomycin, the explants were treated with IL-1b (10 ng/ml) (PeproTech), ANE (10 lM) and ibuprofen (10 lM) (Sigma-Aldrich) for 4 days under serum-free conditions with ITS (insulin-transferrin-selenium; Sigma-Aldrich) according to previous studies [20][21][22][23]. Following 4-day culture, the medium was collected and the explants were fixed in 4% paraformaldehyde.…”

Section: Human Articular Cartilage Explant Culturementioning

confidence: 99%

Anemonin attenuates osteoarthritis progression through inhibiting the activation of IL‐1β/NF‐κB pathway

Wang¹,

Huang²,

Zhou³

et al. 2017

J Cellular Molecular Medi

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The osteoarthritis (OA) progression is now considered to be related to inflammation. Anemonin (ANE) is a small natural molecule extracted from various kinds of Chinese traditional herbs and has been shown to inhibiting inflammation response. In this study, we examined whether ANE could attenuate the progression of OA via suppression of IL‐1β/NF‐κB pathway activation. Destabilization of the medial meniscus (DMM) was performed in 10‐week‐old male C57BL/6J mice. ANE was then intra‐articularly injected into joint capsule for 8 and 12 weeks. Human articular chondrocytes and cartilage explants challenged with interleukin‐1β (IL‐1β) were treated with ANE. We found that ANE delayed articular cartilage degeneration in vitro and in vivo. In particular, proteoglycan loss and chondrocyte hypertrophy were significantly decreased in ANE ‐treated mice compared with vehicle‐treated mice. ANE decreased the expressions of matrix metalloproteinase‐13 (MMP13), A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5), collagen X (Col X) while increasing Aggrecan level in murine with DMM surgery. ANE treatment also attenuated proteoglycan loss in human cartilage explants treated with IL‐1β ex vivo. ANE is a potent protective molecule for OA; it delays OA progression by suppressing ECM loss and chondrocyte hypertrophy partially by suppressing IL‐1β/NF‐κB pathway activation.

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Cyclic Phosphatidic Acid Relieves Osteoarthritis Symptoms

Cited by 17 publications

References 30 publications

Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3

Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3

Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses

Anemonin attenuates osteoarthritis progression through inhibiting the activation of IL‐1β/NF‐κB pathway

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