Cyclic nucleotides affect growth and differentiation of cultured rat embryonic shields

Škreb, N.; Hofman, Lj.; Škreb, Yvette; Suchanek, Ernest; Šerman, Draško

doi:10.1016/0012-1606(84)90150-7

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…Previous results obtained with the serumsupplemented medium have shown that various biologically active substances (for example, cAMP and cGMP) were able to change growth and differentiation of the embryonic part of post-implantation rat embryos in organ culture (34,35). This was also noted after exposure to two teratogens, retinoic acid (14,35) and 5-azacytidine (19).…”

Section: Discussionmentioning

confidence: 98%

Gastrulating Rat Embryo in a Serum-free Culture Model: Changes of Development Caused by Teratogen 5-Azacytidine

et al. 1999

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Developmental processes in gastrulating rat embryos were investigated by using an original, serum-free, chemically defined model system. 9.5-day-old rat embryos, without extraembryonic membranes, were cultivated at the air–liquid interface in a serum-free medium, with and without a protein supplement, for 2 weeks. A teratogenic, demethylating agent, 5-azacytidine, was added to serum-free and protein-free culture medium and to serum-free medium supplemented with human transferrin. A single dose of 5-azacytidine impaired the survival, growth and differentiation of embryos in protein-free medium and serum-free medium with transferrin. In contrast, repeated exposure to 5-azacytidine was required to impair growth in serum-supplemented medium. It was concluded that the activity of 5-azacytidine was easier to detect in a simple, chemically defined medium than in a serum-supplemented medium. This serum-free in vitro method could be useful in screening for teratogenic or embryotoxic substances during gastrulation, the most critical stage of mammalian development.

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Section: Discussionmentioning

confidence: 98%

Gastrulating Rat Embryo in a Serum-free Culture Model: Changes of Development Caused by Teratogen 5-Azacytidine

et al. 1999

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Developmental potential of day 13 porcine embryonic disk under in vitro culture conditions

Silcox

Johnson

1988

In Vitro Cell Dev Biol

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Embryonic disks were microsurgically isolated from adjacent trophoblast tissue and cultured for varying periods in vitro. During the first 24 h of culture, vesicles (1 to 4/disk) composed of mesoderm and endoderm formed from the ventral surface. In the subsequent culture period, the vesicles continued to increase in size and by 96 h in vitro, most originally multivesiculated explants possessed a single vesicle formed by delamination and coalescence of smaller vesicles. Scanning electron microscopy revealed the formation of grooves and ridges in abnormal attempts at differentiation by the embryonic ectoderm. Endoderm comprising the outer tissue layer of the vesicle underwent a gradual alteration in surface morphology during in vitro culture. Initially flat, with a paucity of microvilli, these cells became dome-shaped with an abundance of microvilli. In addition, they became highly secretory as revealed by the presence of numerous secretory droplets at their surface. After culture for periods of up to 10 d, several explants displayed areas containing pulsating tissue, with contractions occurring at a rate of 20 to 30/minute, indicative of mesoderm differentiation. Culture of porcine isolated embryonic disk in vitro should enhance investigations into the regulation of germ layer formation and differentiation and assist in determining the tissue source of conceptus secretory products.

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Age-related changes in the response of chick lens cells during long-term culture to insulin, cyclic AMP, retinoic acid and a bovine retinal extract

Patek

Clayton

1990

Experimental Eye Research

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Cyclic nucleotides affect growth and differentiation of cultured rat embryonic shields

Cited by 3 publications

References 34 publications

Gastrulating Rat Embryo in a Serum-free Culture Model: Changes of Development Caused by Teratogen 5-Azacytidine

Gastrulating Rat Embryo in a Serum-free Culture Model: Changes of Development Caused by Teratogen 5-Azacytidine

Developmental potential of day 13 porcine embryonic disk under in vitro culture conditions

Age-related changes in the response of chick lens cells during long-term culture to insulin, cyclic AMP, retinoic acid and a bovine retinal extract

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