CYCLIC NUCLEOTIDE-GATED ION CHANNELs 14 and 16 Promote Tolerance to Heat and Chilling in Rice

Abstract

Calcium signaling has been postulated to be critical for both heat and chilling tolerance in plants, but its molecular mechanisms are not fully understood. Here, we investigated the function of two closely related cyclic nucleotide-gated ion channel (CNGC) proteins, OsCNGC14 and OsCNGC16, in temperature-stress tolerance in rice (Oryza sativa) by examining their loss-of-function mutants generated by genome editing. Under both heat and chilling stress, both the cngc14 and cngc16 mutants displayed reduced survival rates, higher accumulation levels of hydrogen peroxide, and increased cell death. In the cngc16 mutant, the extent to which some genes were induced and repressed in response to heat stress was altered and some Heat Shock factor (HSF) and Heat Shock Protein (HSP) genes were slightly more induced compared to the wild type. Furthermore, the loss of either OsCNGC14 or OsCNGC16 reduced or abolished cytosolic calcium signals induced by either heat or chilling stress. Therefore, OsCNGC14 and OsCNGC16 are required for heat and chilling tolerance and are modulators of calcium signals in response to temperature stress. In addition, loss of their homologs AtCNGC2 and AtCNGC4 in Arabidopsis (Arabidopsis thaliana) also led to compromised tolerance of low temperature. Thus, this study indicates a critical role of CNGC genes in both chilling and heat tolerance in plants, suggesting a potential overlap in calcium signaling in response to high-and low-temperature stress.

Premature Senescence Leaf 50 Promotes Heat Stress Tolerance in Rice (Oryza Sativa L.)

“…In plants, premature leaf senescence is a major symptom resulting from heat stress. During heat-induced senescence, leaf cells undergo a series of cellular changes including reactive oxygen species (ROS) accumulation, photosynthetic apparatus impairment and cell death (Cui et al 2020;Ivanov et al 2017;Lee et al 2014). Accordingly, plants have developed complex biochemical regulatory mechanisms to response and adapt to heat stress (Samakovli et al 2020;Li et al 2020;Wu et al 2018).…”

Calcium Signaling in Plant Programmed Cell Death

“…In plants, CNGCs are involved in low or high temperature stress and their functions are thought to result from their involvement in Ca 2+ influx. OsCNGC14 and OsCNGC16 play critical roles in heat as well as cold tolerance and are modulators of Ca 2+ signals in response to temperature stress in rice [ 88 ]. Furthermore, their homologs AtCNGC2 and AtCNGC4 in Arabidopsis promote plant growth under chilling and improve freezing tolerance [ 88 ].…”

“…OsCNGC14 and OsCNGC16 play critical roles in heat as well as cold tolerance and are modulators of Ca 2+ signals in response to temperature stress in rice [ 88 ]. Furthermore, their homologs AtCNGC2 and AtCNGC4 in Arabidopsis promote plant growth under chilling and improve freezing tolerance [ 88 ]. Moreover, it was reported that disruption of moss CNGCb and Arabidopsis CNGC2 resulted in a hyper-thermosensitive phenotype, showing that these channels were involved in the control of the plant’s heat shock response (HSR) [ 89 ].…”

“…Salt, temperature, anoxic, heavy metal and mechanic damage stresses are depicted. OSCA1: hyperosmolality-induced [Ca 2+ ](i) increase 1; MCA1: mechanosensitive channel 1; GLRs: glutamate receptor-like channels; AtHMA1: heavy metal transporting ATPase 1; NSCC: nonselective cation channel; CAX: H + /Ca 2+ antiporters; COLD1: chilling-tolerance divergence 1; AtANN1: Ca 2+ - permeable transporter ANNEXIN1; OST1: open stomata 1; RGA1: rice G-protein a subunit 1; VPE: vacuole processing enzymes; JJW: JAV1-JAZ8-WRKY51 complex; JA: jasmonic acid; GIPCs: glycosyl inositol phosphoryl ceramides (based on [ 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , ...…”

Heat stress in Marchantia polymorpha: Sensing and mechanisms underlying a dynamic response

“…Plant CNGCs (plasma membrane‐embedded Cyclic Nucleotide‐gated Ca 2+ Channels) are involved in responses to biotic and abiotic stresses, and in development and fertilization (DeFalco, Moeder, & Yoshioka, 2016; Dietrich, Anschuetz, Kugler, & Becker, 2010; Kaplan, Sherman, & Fromm, 2007; Moeder, Urquhart, Ung, & Yoshioka, 2011). OsCNGC14 and OsCNGC16 modulate calcium signals in response to extreme temperatures and are required for heat and chilling tolerance in rice (Cui et al, 2020).…”