Cyclic mechanical strain regulates the development of engineered smooth muscle tissue

Kim, Byung Soo; Nikolovski, Janeta; Bonadio, Jeffrey; Mooney, David J.

doi:10.1038/13671

Cited by 428 publications

(292 citation statements)

References 33 publications

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“…These improvements in material behavior with mechanical stimulation have been commonly reported for engineered tissues, and are attributed to improved collagen synthesis and organization [12,[23][24][25]. For example, Pins et al found that greater stretch of self-assembled collagen fibers resulted in both improved collagen fibril alignment as measured by TEM as well as higher tangential elastic moduli [25].…”

Section: Discussionmentioning

confidence: 97%

Influence of cyclic strain and decorin deficiency on 3D cellularized collagen matrices

et al. 2008

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Cyclic strain evokes the expression of the small leucine-rich proteoglycans decorin and biglycan in 2-D cultures and native tissues. However, strain dependent expression of these proteoglycans has not been demonstrated in engineered tissues. We hypothesized that the absence of decorin may compromise the effect of cyclic strain on the development of engineered tissues. Thus, we investigated the contribution of decorin to tissue organization in cyclically-strained collagen gels relative to statically-cultured controls. Decorin null (Dcn −/− ) and wild-type murine embryonic fibroblasts were seeded within collagen gels and mechanically conditioned using a Flexcell ® Tissue Train ® culture system. After eight days, the cyclically-strained samples demonstrated greater collagen fibril density, proteoglycan content, and material strength for both cell types. On the other hand, increases in cell density, collagen fibril diameter, and biglycan expression were observed only in the cyclically-strained gels seeded with Dcn −/− cells. Although cyclic strain caused an elevation in proteoglycan expression regardless of cell type, the type of proteoglycan differed between groups: the Dcn −/− cell-seeded gels produced an excess of biglycan not found in the wild-type controls. These results suggest that decorin-mediated tissue organization is strongly dependent upon tissue type and mechanical environment.

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Section: Discussionmentioning

confidence: 97%

Influence of cyclic strain and decorin deficiency on 3D cellularized collagen matrices

et al. 2008

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“…The mechanical properties of synthetic ECM, for example, will regulate the ability of cells to rearrange adhesion peptides presented from the material, 52 and through their control of cell proliferation will regulate transfection of adherent cell populations. 67 Externally applied mechanical signals will also regulate the response of cells to the synthetic ECM 68 and the release of growth factors and subsequent tissue formation. 69 …”

Section: Combining and Integrating Signalingmentioning

confidence: 99%

Regenerative medicine in orthopaedic surgery

Corsi

Schwarz

Mooney

et al. 2007

Journal Orthopaedic Research

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Regenerative medicine holds great promise for orthopaedic surgery. As surgeons continue to face challenges regarding the healing of diseased or injured musculoskeletal tissues, regenerative medicine aims to develop novel therapies that will replace, repair, or promote tissue regeneration. This review article will provide an overview of the different research areas involved in regenerative medicine, such as stem cells, bioinductive factors, and scaffolds. The potential use of stem cells for orthopaedic tissue engineering will be addressed by presenting the current progress with skeletal muscle-derived stem cells. As well, the development of a revascularized massive allograft will be described and will serve as a prototypic model of orthopaedic tissue engineering. Lastly, we will describe current approaches used to design cell instructive materials and how they can be used to promote and regulate the formation of bony tissue. ß

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“…Furthermore, an increase in ultimate tensile strength and tissue stiffness is observed, compared to static control. 21,24,28,29,38,39 In a recently developed bioreactor concept, the Diastolic Pulse Duplicator (DPD), 25 dynamic strains were induced in the heart valve leaflets by applying a dynamic pressure difference over the closed valve. The strain-based conditioning approach was tested by culturing human heart valve leaflets in vitro.…”

Section: Introductionmentioning

confidence: 99%

Real Time, Non-Invasive Assessment of Leaflet Deformation in Heart Valve Tissue Engineering

et al. 2008

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Abstract-In heart valve tissue engineering, most bioreactors try to mimic physiological flow and operate with a preset transvalvular pressure applied to the tissue. The induced deformations are unknown and can vary during culturing as a consequence of changing mechanical properties of the engineered construct. Real-time measurement and control of local tissue strains are desired to systematically study the effects of mechanical loading on tissue development and, consequently, to design an optimal conditioning protocol. In this study, a method is presented to assess local tissue strains in heart valve leaflets during culturing. We hypothesize that local tissue strains can be determined from volumetric deformation. Volumetric deformation is defined as the amount of fluid displaced by the deformed heart valve leaflets in a stented configuration, and is measured, noninvasively, using a flow sensor. A numerical model is employed to relate volumetric deformation to local tissue strains in various regions of the leaflets (e.g. belly and commissures). The flow-based deformation measurement method was validated and its functionality was demonstrated in a tissue engineering experiment. Tri-leaflet, stented heart valves were cultured in vitro and during mechanical conditioning, realistic values for volumetric and local deformation were obtained.

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Cyclic mechanical strain regulates the development of engineered smooth muscle tissue

Cited by 428 publications

References 33 publications

Influence of cyclic strain and decorin deficiency on 3D cellularized collagen matrices

Influence of cyclic strain and decorin deficiency on 3D cellularized collagen matrices

Regenerative medicine in orthopaedic surgery

Real Time, Non-Invasive Assessment of Leaflet Deformation in Heart Valve Tissue Engineering

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