Cyclic lipopeptide profile of three Bacillus subtilis strains; antagonists of Fusarium head blight

Abstract

The objective of the study was to identify the lipopetides associated with three Bacillus subtilis strains. The strains are antagonists of Gibberella zeae, and have been shown to be effective in reducing Fusarium head blight in wheat. The lipopeptide profile of three B. subtilis strains (AS43.3, AS43.4, and OH131.1) was determined using mass spectroscopy. Strains AS43.3 and AS43.4 produced the anti-fungal lipopeptides from the iturin and fengycin family during the stationary growth phase. All three strains produced the lipopeptide surfactin at different growth times. Strain OH131.1 only produced surfactin under these conditions. The antifungal activity of the culture supernatant and individual lipopeptides was determined by the inhibition of G. zeae. Cell-free supernatant from strains AS43.3 and AS43.4 demonstrated strong antibiosis of G. zeae, while strain OH131.1 had no antibiosis activity. These results suggest a different mechanism of antagonism for strain OH131.1, relative to AS43.3 and AS43.4.

Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b

“…Majority of the studied cyclic lipopeptides possessing antifungal activity belong to families of surfactin, iturin, fengycin and their analogues [25,[27][28]. Although, biosynthesis, isolation and properties of certain antibiotics are well-studied in P. polymyxa (being a type-species of Paenibacillus spp.…”

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

“…[12,[117][118][119] For example, purification of LP by HPLC was carried out by reversed phase (RP)-HPLC using a semi-preparative C18 column and 0.1% trifluoroacetic acid/methanol/H 2 O as a mobile phase. The elution was monitored using UV detection at 214 and 280 nm.…”

Microencapsulation of Bacillus subtilis B99-2 and its biocontrol efficiency against Rhizoctonia solani in tomato

“…B. subtilis has been widely used in agriculture for promoting plant growth and biocontrol. Various strains of B. subtilis have the ability to synthesize lipopeptide-type compounds (Dunlap et al, 2011) and antibiotics (Raaijmakers et al, 2002); to form a stable biofilm (Bais et al, 2004) need for antagonistic activity; and to induce systemic resistance (Ongena et al, 2007). B. subtilis isolate B99-2 has a broad-spectrum efficacy against various pathogens, such as R. solani, F. oxysporum, Botrytis cinerea, Didymella bryoniae, and Colletotrichum orbiculare (Wang et al, 2010).…”