Cyclic GMP regulation and function in insects

Morton, David B.; Hudson, Martin L.

doi:10.1016/s0065-2806(02)29001-3

Cited by 26 publications

(50 citation statements)

References 162 publications

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“…This analysis predicted that all three subunits should form NO-insensitive guanylyl cyclases. In addition, Gyc-88E was predicted to form an active enzyme in the absence of additional subunits, whereas Gyc-89Da and Gyc-89Db would require either Gycα-99B or Gyc-88E to form an active enzyme (Morton and Hudson, 2002). The predicted sequence of Gyc-89Db was confirmed from an EST clone and we used RT-PCR to obtain the cDNA for Gyc-88E.…”

Section: Whole-mount In Situ Hybridization Of Embryos and Larval Cnsmentioning

confidence: 94%

“…The samples were then dehydrated in ethanol, cleared in methyl salicylate and mounted in Permount (Fisher Scientific, Fairlawn, NJ, USA). A preliminary sequence analysis of Gyc-88E, Gyc-89Da and Gyc-89Db based on the sequences from the annotated Drosophila genome has previously been described (Morton and Hudson, 2002). This analysis predicted that all three subunits should form NO-insensitive guanylyl cyclases.…”

Section: Whole-mount In Situ Hybridization Of Embryos and Larval Cnsmentioning

confidence: 99%

mentioning

confidence: 99%

“…In addition to the previously studied conventional α and β subunits, the Drosophila genome contains three additional soluble cyclase subunits that have all been predicted to be insensitive to NO (Morton and Hudson, 2002;Morton, 2004). One of these, CG4154, is over 80% identical to MsGC-β3 and is predicted to form active homodimers (Morton and Hudson, 2002). The other two, CG14885 and CG14886, have been predicted to require an additional subunit for activity, potentially forming active heterodimers with either CG4154 or the conventional α subunit, Gycα-99B (Morton and Hudson, 2002).…”

mentioning

confidence: 99%

“…One of these, CG4154, is over 80% identical to MsGC-β3 and is predicted to form active homodimers (Morton and Hudson, 2002). The other two, CG14885 and CG14886, have been predicted to require an additional subunit for activity, potentially forming active heterodimers with either CG4154 or the conventional α subunit, Gycα-99B (Morton and Hudson, 2002). Rather than continue to use the CG numbers to designate these guanylyl cyclases, we propose the following designations based on their chromosomal locations: Gyc-88E for CG4154, Gyc-89Da for CG14885 and Gyc-89Db for CG14886.…”

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confidence: 99%

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Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Langlais

Stewart

Morton

2004

Journal of Experimental Biology

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SUMMARYConventional soluble guanylyl cyclases form α/β heterodimers that are activated by nitric oxide (NO). Recently, atypical members of the soluble guanylyl cyclase family have been described that include the ratβ2 subunit and MsGC-β3 from Manduca sexta. Predictions from the Drosophila melanogaster genome identify three atypical guanylyl cyclase subunits: Gyc-88E (formerly CG4154), Gyc-89Da (formerly CG14885) and Gyc-89Db (formerly CG14886). Preliminary data showed that transient expression of Gyc-88E in heterologous cells generated enzyme activity in the absence of additional subunits that was slightly stimulated by the NO donor sodium nitroprusside (SNP) but not the NO donor DEA-NONOate or the NO-independent activator YC-1. Gyc-89Db was inactive when expressed alone but when co-expressed with Gyc-88E enhanced the basal and SNP-stimulated activity of Gyc-88E, suggesting that they may form heterodimers in vivo. Here,we describe the localization of Gyc-88E and Gyc-89Db and show that they are expressed in the embryonic and larval central nervous systems and are colocalized in several peripheral neurons that innervate trachea, basiconical sensilla and the sensory cones in the posterior segments of the embryo. We also show that there are two splice variants of Gyc-88E that differ by seven amino acids, although no differences in biochemical properties could be determined. We have also extended our analysis of the NO activation of Gyc-88E and Gyc-89Db, showing that several structurally unrelated NO donors activate Gyc-88E when expressed alone or when co-expressed with Gyc-89Db.

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Section: Whole-mount In Situ Hybridization Of Embryos and Larval Cnsmentioning

confidence: 94%

Section: Whole-mount In Situ Hybridization Of Embryos and Larval Cnsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Langlais

Stewart

Morton

2004

Journal of Experimental Biology

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Drosophila gustatory preference behaviors require the atypical soluble guanylyl cyclases

et al. 2011

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The intracellular messenger cGMP has been suggested to play a role in taste signal transduction in both vertebrates and invertebrates. In the present study, we have examined the role of the Drosophila atypical soluble guanylyl cyclases (sGCs), Gyc-89Da and Gyc-89Db, in larval and adult gustatory preference behaviors. We showed that in larvae, sucrose attraction requires Gyc-89Db and caffeine avoidance requires Gyc-89Da. In adult flies, sucrose attraction is unaffected by mutations in either gene whereas avoidance of low concentrations of caffeine is eliminated by loss of either gene. Similar defective behaviors were observed when cGMP increases were prevented by the expression of a cGMP-specific phosphodiesterase. We also showed that both genes were expressed in gustatory receptor neurons (GRNs) in larval and adult gustatory organs, primarily in a non-overlapping pattern, with the exception of a small group of cells in the adult labellum. In addition, in adults, several cells co-expressed the bitter taste receptor, Gr66a, with either Gyc-89Da or Gyc-89Db. We also showed that the electrophysiological responses of a GRN to caffeine were significantly reduced in flies mutant for the atypical sGCs, suggesting that at least part of the adult behavioral defects were due to a reduced ability to detect caffeine.

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Molecular Evolution of the Endocrine System

Bolander

2004

Molecular Endocrinology

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Cyclic GMP regulation and function in insects

Cited by 26 publications

References 162 publications

Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Drosophila gustatory preference behaviors require the atypical soluble guanylyl cyclases

Molecular Evolution of the Endocrine System

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