Cyclic electron transport around photosystems I and II in thylacoids of light-green sectors of variegated Ficus benjamina L. leaves

Matishov, Gennady G; Лысенко, В. С.; Soier, V. G.

doi:10.1134/s0012496610060153

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…At the same time alterations of electron transport organization in chlorophyll-deWcient tissues of some plants can result from the fact that residual chlorophyll of albino areas of variegated tissue originates from chloroplasts of single guard cell pairs. Photosynthetic features and arrangement of electron transport chain of such chloroplasts are the subjects of intensive studies and controversy (Outlaw et al 1979;Zeiger et al 1981;Melis and Zeiger 1982;Lawson 2009;Lysenko et al 2010;Matishov et al 2010). These studies are of interest in terms of both the physiology of chlorophyll-deWcient tissues and mechanisms of electron transport through thylakoid membranes in stomata guard cells that requires more detailed and complex data on their photosynthetic characteristic.…”

Section: Introductionmentioning

confidence: 98%

Fluorescence kinetic parameters and cyclic electron transport in guard cell chloroplasts of chlorophyll-deficient leaf tissues from variegated weeping fig (Ficus benjamina L.)

Лысенко

2011

Planta

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Residual chlorophyll in chlorophyll-deficient (albino) areas of variegated leaves of Ficus benjamina originates from guard cell chloroplasts. Photosynthetic features of green and albino sectors of F. benjamina were studied by imaging the distribution of the fluorescence decrease ratio Rfd within a leaf calculated from maximum (Fm) and steady-state leaf chlorophyll fluorescence (Fs) at 690 and 740 nm. Local areas of albino sectors demonstrated an abnormally high Rfd(740)/Rfd(690) ratio. Fluorescence transients excited in albino sectors at red (640 and 690 nm) wavelengths showed an abrupt decrease of the Rfd values (0.4 and 0.1, correspondingly) as compared with those excited at blue wavelengths (1.7-2.4). This "Red Drop" was not observed for green sectors. Normal and chlorophyll-deficient leaf sectors of F. benjamina were also tested for linear and cyclic electron transport in thylakoids. The tests have been performed studying fluorescence at a steady-state phase with CO(2)-excess impulse feeding, photoacoustic signal generated by pulse light source at wavelengths selectively exciting PSI, fluorescence kinetics under anaerobiosis and fluorescence changes observed by dual-wavelength excitation method. The data obtained for albino sectors strongly suggest the possibility of a cyclic electron transport simultaneously occurring in guard cell thylakoids around photosystems I and II under blue light, whereas linear electron transport is absent or insufficient.

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Section: Introductionmentioning

confidence: 98%

Fluorescence kinetic parameters and cyclic electron transport in guard cell chloroplasts of chlorophyll-deficient leaf tissues from variegated weeping fig (Ficus benjamina L.)

Лысенко

2011

Planta

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“…PAM-fluorometry was used in our modification based on a real-time, fast-Fourier spectrometry to select the measured signal, instead of traditional usage of lock-in amplifier. An analogous modification was previously being applied to process the photoacoustic signals, that allows to use of extremely low measuring light at an acceptable level of signal-to-noise ratio (Matishov et al, 2010;Lysenko, 2012;Lysenko and Varduny, 2013). Besides saturating flashes, we also used far-red light pulses of moderate intensity to assess the resulting changes in the amplitude of the signal from F 0 to the lower values (Fs).…”

Section: Fast Fourier Dual-wavelength Pulse Amplitude Modulation (Pamentioning

confidence: 99%

Far-Red Spectrum of Second Emerson Effect: A Study Using Dual-Wavelength Pulse Amplitude Modulation Fluorometry

Лысенко¹,

Varduny²,

Симонович³

et al. 2014

American Journal of Biochemistry and Biotechnology

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Non-additive enhancement of the photosynthesis excited by simultaneous illumination with far-red light and light of shorter wavelengths is called as "second Emerson effect". Its action spectra are well-known as a photosynthetic yield's dependence on light wavelength in red (630-690 nm) spectral region at a constant-wavelength far-red illumination near 700-715 nm. However, the opposite dependence of the photosynthetic yield's of shorter constant-wavelength light (red or blue) on light wavelength in far-red (690-760 nm) spectral region was never studied. In this study the action spectrum of second Emerson effect was studied using a fast-Fourier dual-wavelength Pulse Amplitude Modulation (PAM) fluorometry. Chlorophyll fluorescence in ailanthus (Ailanthus altissima Mill.) leaves was excited with blue modulated light. Far-red induced decrease of fluorescence (fluorescence shift-FRIFS) was studied in response to illumination of leaves with a background light from 690 to 760 nm (10 nm step), calculating FRIFS = (F 0 -Fs)/F 0 , where F 0 -fluorescence measured without and Fs-with far-red light. Maximum FRIFS was observed at 720 nm (11.8%), but it still remained considerable at 740, 750 nm and a low FRIFS values were revealed at 690 and even at 760 nm. Measurements carried out with blue saturating flashes during and after far-red illumination showed the increase of quantum yield of Photosystem II (PSII), calculated as Fv/Fm at 720 nm background light. FRIFS had lower values under excitation with red modulating light. It is concluded that FRIFS is a result of a photochemical quenching caused by an additional selective far-red excitation of PSI in conditions when PSII is preferably excited by blue light thus leading the PSI to limit non-cyclic electron flow. The contradiction between the known absorption spectra of PSI-light harvesting complex I and the observed action spectrum of second Emerson effect (FRIFS spectrum) is discussed.

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Chloroplast and outside-chloroplast interference of light inside leaves

Moriwaki

Falcioni

Giacomelli

et al. 2023

Environmental and Experimental Botany

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Cyclic electron transport around photosystems I and II in thylacoids of light-green sectors of variegated Ficus benjamina L. leaves

Cited by 4 publications

References 5 publications

Fluorescence kinetic parameters and cyclic electron transport in guard cell chloroplasts of chlorophyll-deficient leaf tissues from variegated weeping fig (Ficus benjamina L.)

Fluorescence kinetic parameters and cyclic electron transport in guard cell chloroplasts of chlorophyll-deficient leaf tissues from variegated weeping fig (Ficus benjamina L.)

Far-Red Spectrum of Second Emerson Effect: A Study Using Dual-Wavelength Pulse Amplitude Modulation Fluorometry

Chloroplast and outside-chloroplast interference of light inside leaves

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