We reported a 15-year-old girl with a history of mild left vesicoureteral reflux who underwent direct radionuclide cystography in our department. Bladder catheterization was mistakenly placed in the vagina. The filling phase showed vagina and uterine cavity which was similar to vesicoureteral reflux. The procedure was repeated with correct catheterization of the bladder and no vesicoureteral reflux was noted.