Cyclic Dinucleotides

Wang, Zhenghua; Ma, Dejun; Xi, Zhen

doi:10.1002/9781119692799.ch2

Cited by 2 publications

(1 citation statement)

References 187 publications

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“…It has widespread consequences for human health, and current research is actively exploring its molecular mechanisms. The convenient one‐flask, gram‐scale synthesis of c‐di‐GMP described here and shown in Figure 1 has facilitated these efforts and has been applied to a variety of analogs (Bartsch et al., 2022; Chen, 2023; Chen et al., 2023; Gao, Ascano, Wu, et al., 2013; Gao, Ascano, Zillinger, et al., 2013; Meehan et al., 2016; Sun et al., 2023; Yan, 2021; Zhang et al., 2013, 2023).…”

Section: Commentarymentioning

confidence: 99%

One‐Flask Synthesis of Cyclic Diguanosine Monophosphate (c‐di‐GMP)

Gaffney

Jones

2023

Current Protocols

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The bacterial signaling molecule cyclic diguanosine monophosphate (c‐di‐GMP) plays a key role in controlling biofilm formation and pathogenic virulence, among many other functions. It has widespread consequences for human health, and current research is actively exploring its molecular mechanisms. The convenient one‐flask, gram‐scale synthesis of c‐di‐GMP described here has facilitated these efforts and has been applied to a variety of analogs. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.

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Section: Commentarymentioning

confidence: 99%

One‐Flask Synthesis of Cyclic Diguanosine Monophosphate (c‐di‐GMP)

Gaffney

Jones

2023

Current Protocols

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Quantitative determination of cyclic guanosine monoposphate (c-GMP) in rat tissues using liquid chromatography and tandem mass spectrometry

Popov,

Balabanyan,

Kolgina

et al. 2023

Fk&Fd

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Relevance. Cyclic 3′,5′-guanosine monophosphate is a secondary intracellular messenger that plays a key role in many physiological processes.Quantitative determination of the level of c-GMP in the tissues of laboratory animals is an urgent task of experimental pharmacology and physiology.Purpose of the study. Development of a method for the quantitative determination of cyclic guanosine monophosphate in various tissues of rats using high performance liquid chromatography with mass spectrometric detection.Methods. The biomaterial was homogenized with deionized water. Extraction of c-GMP from homogenates was performed with methanol, acyclovir was used as an internal standard. Detection of c-GMP and acyclovir was performed using a Sciex QTrap 3200MD mass spectrometer, chromatographic separation was performed using an Agilent Technologies 1260 Inﬁnity II HPLC. The mobile phase was methanol and deionized water.Results. Detection of c-GMP was performed by MRM transitions m/z 346.2/152.1; 346.2/135.1, chromatographic determination of c-GMP was performed in reverse phase mode on an Agilent InﬁnityLab Poroshell 120 EC-C18 4.6×100 mm, 2.7 µm column. The retention time of c-GMP and acyclovir was 7.85 and 7.45 minutes, respectively, the total duration of the chromatographic analysis was 12 minutes. The analytical range of the procedure for determining c-GMP in homogenates was 0.5–1000.0 pmol/ml. The content of c-GMP in the tissues of intact Wistar rats was analyzed using the developed method.Conclusion. The developed bioanalytical HPLC-MS/MS method for the quantitative determination of c-GMP fully complies with the validation requirements. The metrological characteristics of the method make it possible to estimate the content of c-GMP in various tissues of rats with high accuracy.

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Cyclic Dinucleotides

Cited by 2 publications

References 187 publications

One‐Flask Synthesis of Cyclic Diguanosine Monophosphate (c‐di‐GMP)

One‐Flask Synthesis of Cyclic Diguanosine Monophosphate (c‐di‐GMP)

Quantitative determination of cyclic guanosine monoposphate (c-GMP) in rat tissues using liquid chromatography and tandem mass spectrometry

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