In this work, we have investigated the one generator cyclic DNA codes with reverse and reverse complement constraints over the ring R = Z4 +uZ4 +u 2 Z4 with u 3 = 0. Skew cyclic codes with reverse complement constraint are constructed over R. We have also determined a one-to-one correspondence between the elements of the ring R and DNA codons satisfying the Watson-Crick complement. Finally, we have established some examples which satisfy the given constraints.