Cyclic Amplification of the Afterglow Luminescent Nanoreporter Enables the Prediction of Anti‐cancer Efficiency

Abstract

We developed ac yclic amplification method for an organic afterglow nanoreporter for the real-time visualization of self-generated reactive oxygen species (ROS). We promoted semiconducting polymer nanoparticles (PFODBT) as acandidate for emitting near-infrared afterglow luminescence.I ntroduction of ac hemiluminescent substrate (CPPO) into PFODBT (PFODBT@CPPO) resulted in as ignificant enhancement of afterglow intensity through the dual cyclic amplification pathway involving singlet oxygen ( 1 O 2 ). 1 O 2 produced by PFODBT@CPPO induced cancer cell necrosis and promoted the release of damage-related molecular patterns,t hereby evoking immunogenic cell death (ICD)-associated immune responses through ROS-based oxidative stress. The afterglow luminescent signals of the nanoreporter were well correlated with light-driven 1 O 2 generation and anti-cancer efficiency.T his imaging strategy provides an on-invasive tool for predicting the therapeutic outcome that occurs during ROSmediated cancer therapy.

A Photosensitizer Discretely Loaded Nanoaggregate with Robust Photodynamic Effect for Local Treatment Triggers Systemic Antitumor Responses

“…Moreover, ICG-loaded nanosystems also can activate ICD-associated cancer immunotherapy through induction of endoplasmic reticulum stress . Nevertheless, the inherent photobleaching and aggregation-caused quenching (ACQ) properties of ICG considerably reduce its singlet oxygen quantum yield ( Φ Δ ) and hence limit its PDT potency, − causing weak ICD effect and suboptimal treatment of metastases. Nowadays, many strategies ( e.g.…”

Metal-fluorouracil networks with disruption of mitochondrion enhanced ferroptosis for synergistic immune activation

“…Furthermore, immunohistochemical analysis showed that a large amount of CD8 + T cell infiltration within tumor treated by Zn-Fu MNs, compared with PBS group (T cells indicated by arrows). Besides, the representative tumor sections were stained for apoptosis and necrosis analysis using the hematoxylin and eosin (H&E) 62 and terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) 63 . H&E and TUNEL stained tumor slice showed the obvious nuclear condensation and cell contour blurry in H&E observation and increasing green dots in TUNEL images, suggesting severe cell apoptosis and necrosis for tumor treated by Zn-Fu MNs (Figure 5 C).…”

An Activatable Afterglow/MRI Bimodal Nanoprobe with Fast Response to H2S for In Vivo Imaging of Acute Hepatitis

“…We next optimized the incubation conditions of F1-GdNP for the detection of endogenous H 2 Sv ia epifluorescence imaging.T he intracellular fluorescence intensified with the incubation time or probesc oncentration, which reached ap lateau in RAW264.7 macrophages following incubation with F1-GdNP (28 mgmL À1 MEH-PPV) for 3h (Figure S13,14). Colocalization studies revealed ag ood overlap between the fluorescence of activated F1-GdNP at MEH-PPV channel and lysotracker,indicating the main distribution of F1-GdNP in the lysosomes after entry into RAW264.7 cells (Figure S15).…”