Cyclic AMP Prevents Retraction of Axon Terminals in Photoreceptors Prepared for Transplantation: An In Vitro Study

Khodair, Mohamad A.; Zarbin, Marco A.; Townes‐Anderson, Ellen

doi:10.1167/iovs.04-0579

Cited by 22 publications

(11 citation statements)

References 36 publications

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“…Alternatively, FSK may have influenced (i.e., reduced) the amount of mislocalization by preserving retinal structure. 47 In contrast, retinas treated with 10 lM bionone showed increased sprouting as expected but only if the retina was detached and thus had abundant mislocalized opsin (Fig. 7D attached; Fig.…”

Section: Rod Cell Sprouting In Cultured Neural Retinasupporting

confidence: 61%

Mislocalized Opsin and cAMP Signaling: A Mechanism for Sprouting by Rod Cells in Retinal Degeneration

Wang

Zhang

Beuve

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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Section: Rod Cell Sprouting In Cultured Neural Retinasupporting

confidence: 61%

Mislocalized Opsin and cAMP Signaling: A Mechanism for Sprouting by Rod Cells in Retinal Degeneration

Wang

Zhang

Beuve

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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“…NF proteins are degraded by calpains (Schlaepfer and Hasler,1979; Pant et al,1982; Zimmerman and Schlaepfer,1982; Glass et al,1994). The mechanism for this effect is unknown but reduction of axon terminal retraction has been seen in porcine photoreceptors exposed in vitro to either 8‐(4‐chlorophenylthio)‐cAMP or forskolin (Khodair et al,2005). cAMP might inhibit axon retraction by phosphorylating NFs into a form that resists proteolysis by calpains.…”

Section: Discussionmentioning

confidence: 99%

Axon regeneration in the absence of growth cones: Acceleration by cyclic AMP

Jin

Zhang

Jamison

et al. 2009

J of Comparative Neurology

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Regenerative failure of spinal axons is commonly ascribed to signaling of F-actin depolymerization and growth cone collapse by molecules such as the myelin-associated growth inhibitors. cAMP is thought to promote regeneration at least in part by neutralizing this effect, either by direct action in the growth cone or indirectly by transcriptional mechanisms. In vivo evidence for this is based mainly on partial lesion studies in which it is sometimes difficult to distinguish regeneration of injured axons from collateral sprouting by uninjured axons. Moreover, previous observations on fixed lamprey central nervous system (CNS) suggested that regeneration may not involve growth cones. To distinguish actively growing axons from static or retracting ones, fluorescently labeled large reticulospinal axons were imaged in the living, transected lamprey cord with and without application of cAMP analogs and then studied by 2-photon microscopy. Axon tip movements over 2-48-hour intervals indicated: 1) regeneration was intermittent; 2) cAMP decreased initial axon retraction and increased subsequent regeneration up to 11-fold; 3) the increase in regeneration was due to an increase in velocity of axon growth, but not in the time spent in forward movement; 4) tips of actively regenerating axons were more sharply contoured than static tips but no filopodia or lamellipodia were observed, even in db-cAMP; and 5) during active growth, axon tips contained vesicle-like inclusions and were highly immunoreactive for neurofilaments. Staining for F-actin and microtubules was variable and F-actin was not concentrated at the leading edge. Thus, cAMP accelerates regeneration of lamprey spinal axons without inducing formation of growth cones.

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“…32 These explants were randomly assigned to different culture conditions to negate the potential effects of selection bias and variability in retinal thickness. 33 Retinal Cytotoxicity. The CM was centrifuged, frozen, and analyzed for selected trophic factors (Table 1).…”

Section: Porcine Retinamentioning

confidence: 99%

Characterization of Conditioned Media Collected from Cultured Adult versus Fetal Retinal Pigment Epithelial Cells

Kolomeyer¹,

Sugino²,

Zarbin³

2011

Invest. Ophthalmol. Vis. Sci.

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Cyclic AMP Prevents Retraction of Axon Terminals in Photoreceptors Prepared for Transplantation: An In Vitro Study

Abstract: Pharmacological manipulation of photoreceptor axonal plasticity may improve graft-host synaptic interaction after subretinal photoreceptor cell transplantation.

Cited by 22 publications

References 36 publications

Mislocalized Opsin and cAMP Signaling: A Mechanism for Sprouting by Rod Cells in Retinal Degeneration

Mislocalized Opsin and cAMP Signaling: A Mechanism for Sprouting by Rod Cells in Retinal Degeneration

Axon regeneration in the absence of growth cones: Acceleration by cyclic AMP

Characterization of Conditioned Media Collected from Cultured Adult versus Fetal Retinal Pigment Epithelial Cells

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