Cyclic AMP in relation to proliferation of the Epidermal cell: a new view

Green, Howard

doi:10.1016/0092-8674(78)90265-9

Cited by 473 publications

(202 citation statements)

References 37 publications

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“…Additionally, to eliminate cholera toxin from modified KCM, L-isoproterenol, a well-known β-agonist, was used as the replacement. The epidermal CFE of L-isoproterenol had been compared to the CFE of epidermal cells to that of cholera toxin (Green, 1978), and in this study, the effect of L-isoproterenol was confirmed in canine oral mucosal epithelial cell culture.…”

Section: Discussionmentioning

confidence: 78%

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Takagi

Yamato

Murakami

et al. 2010

J Tissue Eng Regen Med

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Keratinocyte culture medium (KCM) has been used for the in vitro culture of keratinocytes and other types of epithelial cells, and the medium includes various ingredients. In this study, two modified KCMs were prepared. In the first, insulin, hydrocortisone and antibiotics that are normally included in KCM were replaced with clinically approved pharmaceutical agents, except transferrin and selenium; in the second, cholera toxin (CT) was replaced by L-isoproterenol (ISO). The modified KCMs were then compared to conventional KCM containing laboratory-grade reagents. Induced cell colony formations of canine oral mucosal epithelial cells cultured in both modified KCMs were found to be nearly equivalent to that in the control KCM, and there was no significant difference between the effect of CT and ISO. Canine oral mucosal cells proliferated to confluence in all three KCM formulations, with or without the use of 3T3 feeder layers. Cultured epithelial cells were harvested from temperature-responsive culture surfaces as an intact cell sheet, and the immunohistochemical analysis of the sheets showed that p63 and cytokeratin were expressed in the epithelial cell sheets cultured in all KCMs. Eventually, in the modified KCM formula, fetal bovine serum was replaced by autologous human serum, and the formula was found to be able to fabricate human oral mucosal epithelial cell sheets. These results indicated that the modified KCM was equally efficient as conventional KCM in the fabrication of transplantable stratified epithelial cell sheets.

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Section: Discussionmentioning

confidence: 78%

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Takagi

Yamato

Murakami

et al. 2010

J Tissue Eng Regen Med

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“…Figure 1, disaggregated cells from rat epidermis and esophagus initiated tightly packed epithelial colonies that pushed back the feeder layer as they expanded. Some esophageal colonies appeared stratified early, but most did not exhibit visible squame formation until reaching moderate size colony size of both cell types increased faster when they were grown in the presence of hydrocortisone (Rheinwald and Green, 1975), epidermal growth factor (Rheinwald and Green, 1977), and cholera toxin (Green, 1978).…”

Section: Methodsmentioning

confidence: 99%

Rat esophageal and epidermal keratinocytes: Intrinsic differences in culture and derivation of continuous lines

Heimann¹,

Rice²

1983

Journal Cellular Physiology

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Serially cultivated with 3T3 feeder layer support as colonies of stratified squamous epithelium, rat epidermal and esophageal epithelial cells were readily distinguishable by three critera. First, the epidermal colonies, exhibiting extensive piling up of squarnes in the centers, were more stratified than esophageal colonies. Second, in sparse culture 70 to 90% of the esophageal cells but as few as 1 to 5% of the epidermal cells were competent in cross‐linked envelope formation upon treatment with the ionophore X537A. After reaching confluence, up to 90% of the cells of both types formed envelopes upon ionophore treatment. Third, epidermal cells in suspension culture reached maximal levels of spontaneously cross‐linked envelopes in 1 day or less, while esophageal cells required about 4 days in suspension to reach maximal levels. A reproducible finding with both cell types was that initial colony‐forming efficiencies of less than 1% increased to about 40% upon serial passage with consequent derivation of continuous lines. Sparse cultures of esophageal cells with high colony‐forming ability retained a high degree of envelope competence (70 to 90%), indicating these two properties are not mutually exclusive. The derived lines exhibited reduced dependence upon feeder layer support at clonal density, but in suspension culture the cells did not grow and lost colony‐forming ability with a half‐time of several hours. We conclude that cells from these keratinized rat epithelia exhibit intrinsic differences in culture and become continuous lines expressing characteristic regulation of envelope competence and loss of germinative capability in suspension.

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“…For many fibroblast and established tumor cell lines, this second messenger is either without effect or is a negative regulator of proliferation (Pastan et al, 1975). In contrast, skin and mammary epithelia (Green, 1978;Yang et al, 1980), hepatocytes (Friedman et al, 1981), thyrocytes (Roger et al, 1983), Schwann cells (Raff et al, 1978a) and melanocytes (Mayer, 1982), among many other cell types, respond to elevation of intracellular cAMP by dividing. For these cells, combined application of cAMP and a polypeptide mitogen, such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), fibroblast growth factor (FGF) or insulin, typically promotes a synergistic (greater than additive) stimulation of cell proliferation (Raff et al, 1978a;McGowan et al, 1981;Roger and Dumont, 1984;Westermark et al, 1986;Roger et al, 1987).…”

Section: Introductionmentioning

confidence: 99%