Cyclic AMP binding proteins in human breast cancer

Miller, William R.; Senbanjo, Richard; Telford, John N.; Watson, David

doi:10.1038/bjc.1985.224

Cited by 13 publications

(5 citation statements)

References 11 publications

(14 reference statements)

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“…All possessed binding activity, levels varying from 267 to 12,037 fmol per mg of cytosol protein (mean value of 4248 fmol mg-'). These results fall within the range of human breast cancer cytosols reported by Miller et al (1985) and are higher than those reported for a series of colorectal cancers using the same methodology. The mean dissociation constant of 2.4 X 10-8 M is also in keeping with data from previous work on human breast cancers (Miller et al, 1985) and work reported for binding proteins in other tumours .…”

Section: Discussionsupporting

confidence: 81%

Cyclic adenosine 3',5'-monophosphate-binding proteins in human ovarian cancers

Ramage

Burns²,

Miller³

1994

Br J Cancer

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Section: Discussionsupporting

confidence: 81%

Cyclic adenosine 3',5'-monophosphate-binding proteins in human ovarian cancers

Ramage

Burns²,

Miller³

1994

Br J Cancer

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“…This may explain why the R/protein values differ in different parts of the same tumour (Miller et al, 1985). Since the amount of C subunit reflects the content of a cell protein, and the ratio between R and C subunits has biological significance, the R/C ratio may be a better parameter than R/protein for clinical purposes.…”

Section: Discussionmentioning

confidence: 99%

“…The ratio between the concentrations of oestrogen receptor and R has appeared useful to predict the response to endocrine therapy. Both for human mammary carcinoma (Kvinnsland et al, 1983;Miller et al, 1985;Watson et al, 1987) and DMBA-induced mammary carcinoma in the rat (Bodwin et al, 1980) this parameter was more reliable than steroid receptor levels alone. Recently, tumour relapse rate and patient survival was found to correlate with the concentration of R subunits in tissue homogenates from human primary mammary tumours (Miller et al, 1990).…”

mentioning

confidence: 96%

Differential expression of cAMP-kinase subunits is correlated with growth in rat mammary carcinomas and uterus

Houge

Cho-Chung²,

Døskeland³

1992

Br J Cancer

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SummaryThe expression of the regulatory (RI and RII) and catalytic (C) subunits of cAMP-dependent protein kinase was found to depend on the growth-state in oestrogen-dependent DMBA-induced mammary adenocarcinomas as well as in uteri of the rat. Castration-induced atrophy of the oestrogen-dependent tissues was accompanied by a decrease of the concentration of regulatory subunits (RI and RII) relative to both the catalytic subunit (C) and total protein, decreasing the R/protein and R/C ratios. A hyperplastic burst caused by high-dose oestrogen-replacement treatment was associated with an increased level of RI and little change in RII and C levels. Only minor differences were noted for the expression of mRNA for the a and P subtypes of RI, RII and C between rat uteri from castrated and oestrogen-treated animals, or between mammary tumours from normal and castrated animals. Expression of RIP-mRNA was detected only in the uterus. Our findings provide an experimental correlate for the reported value of the parameter R/protein in human mammary cancer biopsies to predict prognosis and outcome of therapy. Due to the sensitivity of the R/protein ratio towards changes in extracellular protein content, we recommend the biologically more meaningful R/C ratio in further clinical evaluations of mammary tumour biopsies.

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“…Premenopausal patients with regular menstrual periods (4 women) were treated by oophorectomy. The Cyclic AMP binding was determined as described previously (Miller et al, 1985) and the activity expressed on the basis of cytosol protein which has been reported to reduce intra-tumoral variation (Senbanjo et al, 1986). Concentration of oestrogen receptors was determined (in a portion of tumour adjacent to that taken for cAMP BP) by saturation analysis (Hawkins et al, 1981).…”

mentioning

confidence: 99%