Cyclic AMP and higher plants

Brown, Eric G.; Newton, Russell P.

doi:10.1016/0031-9422(81)83071-3

Cited by 116 publications

(58 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, extensive investigations were required to positively identify cyclic AMP and cyclic GMP in plant tissues (2,21). Furthermore, unpurified tissue extracts from Phycomyces blakesleeanus contained a component which inhibited the binding of cyclic AMP to its antibody in the cyclic AMP assay (20).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Intracellular and Extracellular Cyclic Nucleotides in Wild-Type and White Collar Mutant Strains of Neurospora crassa

Shaw¹,

Harding²

1987

Plant Physiol.

View full text Add to dashboard Cite

Cyclic AMP and cyclic GMP were released into the growth medium of mycelia of Neurospora crassa wild-type strains St.L.74A and Em5297a and by white collar-i and white collar-2 mutant strains. After growth for 6 days at 18°C, there were 2.19 (St.L.74A), 5.83 (Em5297a), 1.38 (white collar-i), and 1.10 (white collar-2) nanomoles of cyclic AMP per gram dry weight of mycelia in the growth medium. These values corresponded to concentrations of cyclic AMP of between approximately 10 and 50 nanomolar. The corresponding values for extracellular cyclic GMP were typically less than 6% of the values for cyclic AMP. Following transfer to fresh medium, cyclic AMP efflux was demonstrated for each of the strains, and the amount of cyclic AMP exported into the fresh medium was greater at 25°C than 6°C. Intracellular cyclic AMP and cyclic GMP were also measured in each of the strains. The values for cyclic AMP were in the same range as those in the literature (approximately 0.5 to 1.5 nanomoles per gram dry weight of mycelia). However, the corresponding intracellular cyclic GMP values were less than 1% of the cyclic AMP values, i.e. more than 50 times lower than the value previously reported for the St.L.74A wild-type. Transfer of mycelia after 6 days at 18°C to fresh media and incubation for 2 hours at 25°C or 6°C did not consistently affect the intracellular level of cyclic AMP or cyclic GMP in the strains examined. We could detect no change in intracellular cyclic AMP when mycelia of the St.L.74A wild-type strain were irradiated with blue light for periods of up to 3.0 hours at 180 C, or in cyclic AMP and cyclic GMP for irradiation times of up to 1 minute at 6°C. We propose that the plasma membrane of Neurospora crassa is permeable to cyclic nucleotides, and the export of cyclic nucleotides into the growth medium may be a means of regulating intracellular levels. We conclude that three factors that affect carotenogenesis in Neurospora crassa (blue light, temperature, and the white collar mutations) have no appreciable effect on the total measurable intracellular cyclic nucleotides in this organism. There was no extracellular or intracellular cyclic AMP or cyclic GMP in the crisp-i mutant strain, which suggested either that adenylate cyclase (which is absent in cnisp-i) catalyzes the synthesis of both cyclic AMP and cyclic GMP or that the cnisp-i mutation somehow results in a deficiency of two enzymes (adenylate and guanylate cyclase).Cyclic AMP and cyclic GMP have important regulatory roles in the metabolism of animals, fungi, bacteria, lower plants, and possibly higher plants. In Francko [10]). In addition, in the slime mold Dictyostelium discoideum, extracellular cyclic AMP is involved in cellular interaction (for a review see Devreotes [9]).In Neurospora crassa the presence of cyclic GMP has been reported (25), and cyclic AMP may be involved in morphological development (24). The crisp-i morphological mutants can be restored to wild-type morphology by adding cyclic AMP to their growth medium (25,28). Work in this...

show abstract

Section: Discussionmentioning

confidence: 99%

“…A unifying hypothesis suggesting that cyclic AMP stimulates glycolysis in fungi has been proposed (24), whereas in bacteria cyclic AMP is involved in the control ofgene expression (1 1). Although the presence of cyclic AMP (2) and cyclic GMP (21) in higher plants has been rigorously proven, their functions remain unknown.…”

mentioning

confidence: 99%

Intracellular and Extracellular Cyclic Nucleotides in Wild-Type and White Collar Mutant Strains of Neurospora crassa

Shaw¹,

Harding²

1987

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Although a range of physiological processes and enzymatic reactions in plants were suggested to be responsive to cAMP (Brown & Newton, 1981 ;Newton & Brown, 1986), for credibility to be sustainable it was essential to demonstrate unequivocally the identity of the putative cAMP obtained in tissue and cell extracts and as the product of incubations with adenylyl cyclase. This was successfully accomplished by the use of physical techniques including MS and NMR spectroscopy, as detailed in section III ; the inclusion of suitable controls has also been used to demonstrate that the identified cAMP is not an artefact.…”

Section:       C   mentioning

confidence: 99%

“…A large number of physiological processes in plants are potentially sensitive to alterations in [cAMP], as suggested by reports of the effects of cell-permeating cAMP analogues and of alterations in [cAMP] and cAMP-related enzyme activities during physiological events (Brown & Newton, 1981 ;Newton & Brown, 1986 ;Assmann, 1995 ;Bolwell, 1995). However, it is questionable whether many of these observations have any relevance in vivo.…”

Section:    C mentioning

confidence: 99%

Tansley Review No. 106

et al. 1999

Self Cite

View full text Add to dashboard Cite

 For three decades, hypotheses relating to the occurrence and function of cyclic nucleotides in higher plants have been highly controversial. Although cyclic nucleotides had been shown to have key regulatory roles in animals and bacteria, investigations with higher plants in the 1970s and early 1980s were criticized on the basis of (i) a lack of specificity of effects apparently elicited by cyclic nucleotides, (ii) the equivocal identification of putative endogenous cyclic nucleotides and (iii) ambiguity in the identification of enzymes connected with cyclic nucleotide. More recent evidence based on more rigorous identification procedures has demonstrated conclusively the presence of cyclic nucleotides, nucleotidyl cyclases and cyclic nucleotide phosphodiesterases in higher plants, and has identified plant processes subject to regulation by cyclic nucleotides. Here we review the history of the debate, the recent evidence establishing the presence of these compounds and their role ; future research objectives are discussed.

show abstract

“…We are aware of the basic criticism of many authors with regard to the possibility of a regulatory system based on cAMP in plants (AMRHEIN 1977); nevertheless, we considered worth attempting to test the effect of cAMP on the cell cycle of root meristems in view of the recent well-founded data obtained by Brown's group (BROWN and NEWTON 1981) and others (JANISTYN 1981;JoHNSON et al 1981) on the presence of a cAMP system also in plants.…”

Section: Introductionmentioning

confidence: 99%

Arrest of the Cell Cycle Induced by Dibutyryl-Camp and Sodium Butyrate in Root Meristems of Lettuce

1986

View full text Add to dashboard Cite

SUMMARY -Dibutyryl-cAMP was supplied to seedlings of Lactuca sativa L. cv.Great Lakes 118, and its effect on the cell cycle in root meristems was compared to that of dibutyryl-cGMP and sodium butyrate. Dibutyryl-cAMP and sodium butyrate induced a sharp decrease in the labelling index, attributable to an arrest of the cell cycle in G1 and possibly G2, whereas dibutyryl-cGMP was ineffective. The use of dibutyryl-cAMP and sodium butyrate in studies on the control of cell cycle and cell differentiation in growing roots is suggested. Various hypotheses on the mode of action of dibutyryl-cAMP are also discussed.

show abstract

Cyclic AMP and higher plants

Cited by 116 publications

References 106 publications

Intracellular and Extracellular Cyclic Nucleotides in Wild-Type and White Collar Mutant Strains of Neurospora crassa

Intracellular and Extracellular Cyclic Nucleotides in Wild-Type and White Collar Mutant Strains of Neurospora crassa

Tansley Review No. 106

Arrest of the Cell Cycle Induced by Dibutyryl-Camp and Sodium Butyrate in Root Meristems of Lettuce

Contact Info

Product

Resources

About