Cyclic Adenosine 3′,5′-Monophosphate Concentration in Plasma, Adipose Tissue and Skeletal Muscle in Normal Subjects and in Patients with Hyper- and Hypothyroidism

Karlberg, Bengt E.; Henriksson, K. G.; Andersson, Rolf G. G.

doi:10.1210/jcem-39-1-96

Cited by 61 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…' The recent observation that thyroid hormones inhibit phosphodiesterase in fetal rat calvaria has raised the possibility that thyroid hormones stimulate bone resorption by raising the intracellular concentrations of cyclic AMP (20). This hypothesis is supported by the observation that PTH increases intracellular cyclic AMP concentrations in bone cells (26), and that plasma and urine cyclic AMP concentrations are increased in both hyperparathyroidism and hyperthyroidism (27). Phosphodiesterase inhibition by dibutyryl cyclic AMP (28) and theophylline is associated with increased cyclic AMP concentrations and bone resorption, and the possibility that thyroid hormones caused bone resorption via this mechanism was considered.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Direct stimulation of bone resorption by thyroid hormones.

Mundy¹,

Shapiro²,

Bandelin³

et al. 1976

J. Clin. Invest.

349

127

View full text Add to dashboard Cite

A B S T R A C T Although hypercalcemia, osteoporosis, and increased bone turnover are associated with thyrotoxicosis, no direct effects of thyroid hormones on bone metabolism have been reported previously in organ culture. We have now demonstrated that prolonged treatment with thyroxine (T4) or triiodothyronine (T3) can directly increase bone resorption in cultured fetal rat long bones as measured by the release of previously incorporated 'Ca. T4 and T3 at 1 ;M to 10 nM increased 'Ca release by 10-60% of total bone 'Ca during 5 days of culture. The medium contained 4 mg/ml of bovine serum albumin to which 90% of T4 and T3 were bound, so that free concentrations were less than 0.1 uM. The response to T4 and T3 was inhibited by cortisol (1 AM) and calcitonin (100 mU/ml). Indomethacin did not inhibit T4 response suggesting that T4 stimulation of bone resorption was not mediated by increased prostaglandin synthesis by the cultured bone. Matrix resorption was demonstrated by a decrease in extracted dry weight and hydroxyproline concentration of treated bones and by histologic examination which also showed increased osteoclast activity. The effects of thyroid hormones were not only slower than those of other potent stimulators of osteoclastic bone resorption (parathyroid hormone, vitamin D metabolites, osteoclast activating factor, and prostaglandins), but the maximum response was not as great. We conclude that T4 and Ts can directly stimulate bone resorption in vitro at concentrations approaching those which occur in thyrotoxicosis. This effect may explain the disturbances of calcium metabolism seen in hyperthyroidism.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Unpublished observations. culating concentrations of cyclic AMP because of indirect mechanisms, for example increased sensitivity of beta-adrenergic receptors to catecholamines (27).…”

Section: Resultsmentioning

confidence: 99%

Direct stimulation of bone resorption by thyroid hormones.

Mundy¹,

Shapiro²,

Bandelin³

et al. 1976

J. Clin. Invest.

349

127

View full text Add to dashboard Cite

show abstract

“…On the other hand, during the third trimester, T3 is more elevated and most likely its unbound fraction too, compen sating an inadequate increase of thyroxin at maximum amounts of TBG, in order to keep the euthyroid state (Kaiman et al, 1973;Rastogi et al, 1974;Winikoff and Malinek, 1975). These elevated T3 concentrations might elevate cAMP levels in tissues and plasma by way of increased adenylcyclase activity by (3-receptor stimulation (Karlberg et al, 1974). But a significant negative correlation be tween T3 and cAMP was found.…”

Section: Commentmentioning

confidence: 99%

Plasma cAMP in Normal and Abnormal Human Pregnancy

Göser¹,

Jaschonek²,

Kindler³

et al. 1980

Gynecol Obstet Invest

View full text Add to dashboard Cite

Plasma cAMP was determined using the method of Toveyetal. in normal pregnant women with a mean concentration of 18.9 ± 0.8 pmol/ml (x ± SEM). Between weeks 9–12 and 33–36 of gestation, there were two peaks, with a mean cAMP of 22.5 ± 2.4, which were significantly increased in comparison to the other weeks of pregnancy. Significantly decreased values were found in patients with threatened abortion (weeks 12–28) which terminated in abortion (11.6 ± 2.4; p < 0.01). In premature labor no differences were found. During therapy with fenoterol there were highly significantly increased plasma cAMP levels (48.2 ± 2.8; p < 0.0005). During thyroid hormone therapy in euthyroid goiter, cAMP was significantly decreased (14.0 ± 1.4; p < 0.05). 1 week after cessation of therapy a highly significant increase of cAMP was observed (38.2 ± 6.9; p < 0.0005). There was a negative linear regression between T₃ and cAMP ( 2p < 0.01). In pregnancy with hypertension cAMP was significantly elevated (30.5 ± 3.8; p < 0.0005), but nearly normal under antihypertensive therapy. In pregnancy with edema only, no difference was found. Induction of labor with PGE₂_Α was followed by a decrease of plasma cAMP.

show abstract

“…In hyperthyroid patients, cyclic AMP lev els were elevated in plasma, skeletal muscle and adipose tissue [13][14][15][16]. Plasma cyclic AMP concentration is probably a reflection of increased intracellular nucleotide generation or production rate [17], Thyroid hormone modulates the response of multiple hormonal effects mediated by cyclic AMP [16].…”

Section: Resultsmentioning

confidence: 99%

Serum Cyclic 3',5'-Nucleotide Phosphodiesterase in Patients with Various Thyroid Disorders

Nagasaka¹,

Hasegawa²,

Hayami³

et al. 1985

Enzyme

View full text Add to dashboard Cite

Previous observations that cyclic 3',5'-nucleotide phosphodiesterase activity exists in mammalian sera including human serum prompted us to investigate the phosphodiesterase levels in sera of patients with various thyroid disorders. Both serum cyclic AMP phosphodiesterase (cAMP-PDE) and cyclic GMP phosphodiesterase (cGMP-PDE) activities measured in a low substrate concentration were elevated 3-fold in subacute thyroiditis and slightly in hyperthyroidism, compared to the normal. Slight decreases of these enzyme activities were observed in primary hypothyroidism. PDE activities were positively correlated with the value of T(3)-RSU and serum thyroid hormone levels in hyper- and hypothyroidism. Altered enzyme activities returned to normal during the course of recovery. Identical results were obtained when plasma was tested. These results suggest that serum PDE activities may be partly related to the thyroid function.

show abstract

Cyclic Adenosine 3′,5′-Monophosphate Concentration in Plasma, Adipose Tissue and Skeletal Muscle in Normal Subjects and in Patients with Hyper- and Hypothyroidism

Cited by 61 publications

References 0 publications

Direct stimulation of bone resorption by thyroid hormones.

Direct stimulation of bone resorption by thyroid hormones.

Plasma cAMP in Normal and Abnormal Human Pregnancy

Serum Cyclic 3',5'-Nucleotide Phosphodiesterase in Patients with Various Thyroid Disorders

Contact Info

Product

Resources

About