Cyclic 3â², 5â²-Guanosine Monophosphate Synthesis Induced by Atrial Natriuretic Peptide, C-Type Natriuretic Peptide, and Nitric Oxide in the Rat Retina

Moriwaki, Yuhei; Kamisaki, Yoshínori; Itoh, Tadao; Nagata, Masao; Tamai, A

doi:10.1016/s0021-5155(98)00009-4

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…On the other hand, the level found in vitreous fluid may not actually represent local levels found at the tissue or cell level. When expressed in picomoles per milligram protein, our intracellular cGMP levels are comparable with cGMP levels found earlier in the rat retina [25] . In our previous study, the mean cGMP level in subretinal fluid from eyes with a retinal detachment was 2.4 n M [17] .…”

Section: Discussionsupporting

confidence: 88%

“…Of the two currently known pathways involved in cGMP production it is evident that production via the particulate guanylyl cyclase pathway results in much higher levels than via the soluble guanylyl cyclase pathway. Previous reports comparing both enzymatic pathways involved in the cGMP production in the rat retina show that the NO-cGMP pathway (via soluble guanylyl cyclase) exists dominantly in the inner nuclear layer and the ANP-cGMP pathway (via particulate guanylyl cyclase) exists dominantly in the rod photoreceptor [6,11,24,25] .…”

Section: Discussionmentioning

confidence: 96%

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Cyclic GMP Synthesis by Human Retinal Pigment Epithelial Cells Is Mainly Mediated via the Particulate Guanylyl Cyclase Pathway

et al. 2006

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Background/Aims: Cyclic 3′,5′-guanosine monophosphate (cGMP), a central molecule in the phototransduction cascade, is also involved in a number of other physiological processes in the retina, like stimulating the absorption of subretinal fluid by activating the retinal pigment epithelium (RPE) cell pump. The aim of this study was to quantify cGMP synthesis by RPE cells and to investigate the role of two separate enzymatic pathways (soluble versus particulate guanylyl cyclase) in its production. Methods: cGMP expression was evaluated by immunochemistry and radioimmunoassay following culture of the D407 RPE cell line in the presence of a nonselective phosphodiesterase inhibitor (IBMX), in combination with the particulate guanylyl cyclase stimulator atrial natriuretic peptide (ANP) or the soluble guanylyl cyclase stimulator sodium nitroprusside (SNP). Results: Stimulation of the particulate guanylyl cyclase in RPE cells with ANP resulted in high intra- and extracellular cGMP levels. Stimulation of the soluble guanylyl cyclase by SNP resulted in a slight elevation of cGMP levels compared to controls. Conclusions: These results show that cultured human RPE cells are capable of producing cGMP and that most cGMP is generated following stimulation of the particulate guanylyl cyclase pathway.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 96%

Cyclic GMP Synthesis by Human Retinal Pigment Epithelial Cells Is Mainly Mediated via the Particulate Guanylyl Cyclase Pathway

et al. 2006

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“…NPs and NPRs are widely distributed in the CNS (DiCicco-Bloom et al, 2004;Dumont et al, 2004), suggesting possible involvement of NPs in neuromodulation in CNS. Localization of NPs in the retina also has been reported in several species (Stone and Glembotski, 1986;Shyjan et al, 1992;Palm et al, 1994;Wolfensberger et al, 1994;Fernandez-Durango et al, 1995;Moriwaki et al, 1998;Haverkamp et al, 1999;Spreca et al, 1999;Blute et al, 2000;Cao et al, 2004;Rollin et al, 2004). There is also evidence suggesting the existence of NPRs in retinal neurons (Blute et al, 2000;Rollin et al, 2004).…”

Section: Introductionmentioning

confidence: 65%

Modulation by Brain Natriuretic Peptide of GABA Receptors on Rat Retinal ON-Type Bipolar Cells

Yu¹,

Cao²,

Yang³

2006

J. Neurosci.

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“…In an effort to assess the potential involvement of membrane guanylyl cyclases in the elevated nocturnal generation of cGMP, we tested atrial natriuretic peptide and the related C‐type natriuretic peptide for their ability to stimulate in vitro cGMP synthesis in the chick pineal. In light of recent studies reporting expression of natriuretic peptide receptors in the retina (29, 30), and based on our earlier work demonstrating expression of both the cognate natriuretic peptide receptors in the mammalian pineal organ (15–17), it was hypothesized that at least one of these receptors ought to be functional in the chick pineal.…”

Section: Discussionmentioning

confidence: 99%

Nocturnal Accumulation of Cyclic 3′,5′‐guanosine Monophosphate (cGMP) in the Chick Pineal Organ is Dependent on Activation of Guanylyl Cyclase‐B

Olcese

Majora

Stephan

et al. 2002

J Neuroendocrinology

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The role of cGMP in the avian pineal is not well understood. Although the light-sensitive secretion of melatonin is a well-known output of the circadian oscillator, pharmacologically elevated cGMP levels do not result in altered melatonin secretory amplitude or phase. This suggests that pineal cGMP signalling does not couple the endogenous circadian oscillator to the expression of melatonin rhythms. Nonetheless, the free-running rhythm of cGMP signalling implies a link to the circadian oscillator in chick pinealocytes. As the circadian rhythm of cGMP levels in vitro is not altered by pharmacological inhibition of phosphodiesterase activity, we infer that the synthesis, rather than the degradation of cGMP, is under circadian control. In vitro experiments with the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine as well as with an inhibitor of the NO-sensitive soluble guanylyl cyclase (sGC), showed that the NOS-sGC pathway does not play a major role in the circadian control of cGMP generation. In organ culture experiments, we demonstrated that C-type natriuretic peptide (CNP), but not atrial natriuretic peptide (ANP), elevated daytime levels of cGMP. As CNP acts on the membrane guanylyl cyclase isoform B (GC-B), which is expressed at very high levels in mammalian pineals, we investigated the effect of the membrane GC-specific inhibitor HS-142 on chick pineal cGMP levels. CNP-induced daytime cGMP levels were reduced by HS-142. More importantly, 'spontaneously' high nocturnal levels of cGMP in vitro were reduced to daytime levels by acute addition of HS-142. These data implicate endogenous nocturnal CNP release and subsequent activation of GC-B as the major input driving cGMP synthesis in the chick pineal organ.

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Cyclic 3â², 5â²-Guanosine Monophosphate Synthesis Induced by Atrial Natriuretic Peptide, C-Type Natriuretic Peptide, and Nitric Oxide in the Rat Retina

Cited by 6 publications

References 28 publications

Cyclic GMP Synthesis by Human Retinal Pigment Epithelial Cells Is Mainly Mediated via the Particulate Guanylyl Cyclase Pathway

Cyclic GMP Synthesis by Human Retinal Pigment Epithelial Cells Is Mainly Mediated via the Particulate Guanylyl Cyclase Pathway

Modulation by Brain Natriuretic Peptide of GABA Receptors on Rat Retinal ON-Type Bipolar Cells

Nocturnal Accumulation of Cyclic 3′,5′‐guanosine Monophosphate (cGMP) in the Chick Pineal Organ is Dependent on Activation of Guanylyl Cyclase‐B

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Cyclic 3â², 5â²-Guanosine Monophosphate Synthesis Induced by Atrial Natriuretic Peptide, C-Type Natriuretic Peptide, and Nitric Oxide in the Rat Retina

Cited by 6 publications

References 28 publications

Cyclic GMP Synthesis by Human Retinal Pigment Epithelial Cells Is Mainly Mediated via the Particulate Guanylyl Cyclase Pathway

Cyclic GMP Synthesis by Human Retinal Pigment Epithelial Cells Is Mainly Mediated via the Particulate Guanylyl Cyclase Pathway

Modulation by Brain Natriuretic Peptide of GABA Receptors on Rat Retinal ON-Type Bipolar Cells

Nocturnal Accumulation of Cyclic 3′,5′‐guanosine Monophosphate (cGMP) in the Chick Pineal Organ is Dependent on Activation of Guanylyl Cyclase‐B

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Cyclic 3â², 5â²-Guanosine Monophosphate Synthesis Induced by Atrial Natriuretic Peptide, C-Type Natriuretic Peptide, and Nitric Oxide in the Rat Retina