Cyberlindnera xylosilytica sp. nov., a xylitol-producing yeast species isolated from lignocellulosic materials

Cadete, Raquel M.; Cheab, Monaliza A M; Santos, Renata; Safar, Silvana V. B.; Zilli, Jerri Édson; Vital, Marcos José Salgado; Basso, Luiz Carlos; Lee, Ching-Fu; Kurtzman, Cletus P.; Lachance, Marc‐André; Rosa, Carlos A.

doi:10.1099/ijs.0.000363

Cited by 19 publications

(25 citation statements)

References 21 publications

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“…Among the species studied by Junyapate et al (2014), C. tropicalis produced the highest concentration of xylitol (7.2 g.L -1 ). Cadete et al (2015) evaluated the production of xylitol from 50 g.L -1 of xylose from five strains of the species Cyberlindnera xylosilytica sp. The maximum production of xylitol was reached in 72 h, which was practically the same among the isolates, with a mean production of 33.02 g.L -1 .…”

Section: Discussionmentioning

confidence: 99%

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Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Souza¹,

Valentim²,

Nogueira³

et al. 2017

Afr. J. Microbiol. Res.

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Beetles of the Passalidae family live and feed on decaying wood and their guts are richly colonized by yeasts. The goal of this research was to prospect xylolytic yeasts with potential for the production of second-generation bioethanol. Therefore, 83 specimens of beetles belonging to the Passalidae and the Scarabaeidae families were collected in the Amazonian rainforest in Itacoatiara -AM, Brazil. Morphological differences of the beetles were identified and 25 chosen specimens were dissected. Yeasts from galleries inhabited by beetles and from insect guts were isolated. Isolates were previously selected through tolerance tests for temperature, ethanol and xylose assimilation capacity. Those isolates were then submitted to a panel of conditions related to ethanol production. The ethanol production reached 24.70 g.L -1 and the xylitol production reached 21.66 g.L -1. One of the isolates with a promising profile was identified as Spathaspora roraimanensis and six as Spathaspora passalidarum. Three isolates showed to be more promising and, curiously, all came from the gut of the species Popilius marginatus (Percheron, 1835). In plate testing, however, the isolates obtained from galleries showed a greater capacity to assimilate xylose. As reported in this field of study, no isolate tolerated all conditions tested. Wild isolates with this profile may be used for testing larger-scale ethanol production, genetic engineering, or evolutionary techniques.

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Section: Discussionmentioning

confidence: 99%

“…In fact, the gut of beetles and other insects is considered a hotspot of yeast diversity (Suh and Blackwell, 2004;Boekhout, 2005;Rivera et al, 2009;Cadete et al, 2009Cadete et al, , 2012Urbina et al, 2013;Gouliamova et al, 2015;Cadete et al, 2015Cadete et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Souza¹,

Valentim²,

Nogueira³

et al. 2017

Afr. J. Microbiol. Res.

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“…Methods for the isolation of yeast strains have been reported previously by Cadete et al 25 . The yeast strains were isolated from tree bark, decaying wood, sugarcane filter cake, insect frass, water, and cactus samples 24,30,32,33 . These isolates were stored in the Collection of Microorganisms and Cells of the Federal University of Minas Gerais (a public microbial collection) at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Wickerhamomyces genera have also been investigated as potential producers of xylitol. C yberlindnera xylosilytica has been reported to give a high yield of xylitol (0.726 g g −1 , with an average production equal to 33 g L −1 ) containing xylose (50 g L −1 ) as a carbon source 30 . Junyapate et al 31 .…”

Section: Introductionmentioning

confidence: 99%

“…reported that Wickerhamomyces rabaulensis is a xylitol‐producing yeast (0.36 g g −1 ) that utilizes xylose (10 g L −1 ) as the carbon source. During studies on yeasts associated with the rotting wood in Brazil's forest ecosystems, we isolated several novel strains of C. xylosilytica and W. rabaulensis 24,30,32,33 . Until now, these strains have not been assessed for their potential to produce xylitol.…”

Section: Introductionmentioning

confidence: 99%

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Novel potential yeast strains for the biotechnological production of xylitol from sugarcane bagasse

Palladino

Rodrigues

Cadete

et al. 2021

Biofuels Bioprod Bioref

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This study aimed to screen the xylitol‐producing strains of Cyberlindnera xylosilytica and Wickerhamomyces rabaulensis for the bioconversion of the hemicellulosic fraction of sugarcane bagasse. Among the 16 strains tested, two of each species (C. xylosilytica UFMG‐CM‐Y309 and UFMG‐CM‐Y409, and W. rabaulensis UFMG‐CM‐Y3716 and UFMG‐CM‐Y3747) were selected on the basis of xylitol yield (Yp/s) and xylitol volumetric productivity (Qp) in YPX broth (yeast extract, 10 g L−1; peptone, 20 g L−1; and d‐xylose, 60 g L−1). These strains were cultivated in sugarcane bagasse hemicellulosic hydrolysate (SCHH) supplemented with ammonium sulfate, rice bran extract, and yeast extract. The xylitol yield was the highest in the strain C. xylosilytica UFMG‐CM‐Y‐309 with a production of 14.06 g L−1; xylitol yield, 0.63 g g−1; xylitol volumetric productivity, 0.20 g L−1 h−1. Xylitol yields ranged from 14% to 74% above those of other xylitol‐production yeast species. This work demonstrates the potential of biotechnological application of these yeasts for xylitol production. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species

Barros

Alvarenga

Magni

et al. 2023

Yeast

Self Cite

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This study investigated the diversity of yeast species associated with rotting wood in Brazilian Amazonian rainforests. A total of 569 yeast strains were isolated from rotting wood samples collected in three Amazonian areas (Universidade Federal do Amazonas-Universidade Federal do Amazonas [UFAM], Piquiá, and Carú) in the municipality of Itacoatiara, Amazon state. The samples were cultured in yeast nitrogen base (YNB)-D-xylose, YNB-xylan, and sugarcane bagasse and corncob hemicellulosic hydrolysates (undiluted and diluted 1:2 and 1:5). Sugiyamaella was the most prevalent genus identified in this work, followed by Kazachstania.The most frequently isolated yeast species were Schwanniomyces polymorphus, Scheffersomyces amazonensis, and Wickerhamomyces sp., respectively. The alpha diversity analyses showed that the dryland forest of UFAM was the most diverse area, while the floodplain forest of Carú was the least. Additionally, the difference in diversity between UFAM and Carú was the highest among the comparisons. Thirty candidates for new yeast species were obtained, representing 36% of the species identified and totaling 101 isolates. Among them were species belonging to the clades Spathaspora, Scheffersomyces, and Sugiyamaella, which are recognized as genera with natural xylose-fermenting yeasts that are often studied for biotechnological and ecological purposes. The results of this work showed that rotting wood collected from the Amazonian rainforest is a tremendous source of diverse yeasts, including candidates for new species.

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Cyberlindnera xylosilytica sp. nov., a xylitol-producing yeast species isolated from lignocellulosic materials

Cited by 19 publications

References 21 publications

Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Novel potential yeast strains for the biotechnological production of xylitol from sugarcane bagasse

The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species

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