CyanoTag: Discovery of protein function facilitated by high-throughput endogenous tagging in a photosynthetic prokaryote
Abigail J. Perrin,
Matthew Dowson,
Adam A. Dowle
et al.
Abstract:Despite their fundamental importance to aquatic ecosystems, global carbon cycling and exciting potential in sustainable bioindustries, the genomes of photosynthetic prokaryotes still contain large numbers of uncharacterised protein-coding genes. Here we present a high-throughput approach for scarless endogenous fluorescent protein tagging in the model cyanobacteriumSynechococcus elongatusPCC7942. From 400 targets we successfully endogenously tag over 330 proteins corresponding to >10% of the proteome. We de… Show more
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