Cyanopeptolins and Anabaenopeptins Are the Dominant Cyanopeptides from Planktothrix Strains Collected in Canadian Lakes

Abstract

Common bloom-forming cyanobacteria produce complex strain-specific mixtures of secondary metabolites. The beneficial and toxic properties of these metabolite mixtures have attracted both research and public health interest. The advancement of mass spectrometry-based platforms and metabolomics data processing has accelerated the identification of new metabolites and feature dereplication from microbial sources. The objective of this study was to use metabolomics data processing to decipher the intracellular cyanopeptide diversity of six Planktothrix strains collected from Canadian lakes. Data-dependent acquisition experiments were used to collect a non-targeted high-resolution mass spectrometry dataset. Principal component analysis and factor loadings were used to visualize cyanopeptide variation between strains and identified features contributing to the observed variation. GNPS molecular networking was subsequently used to show the diversity of cyanopeptides produced by the Planktothrix strains. Each strain produced a unique mixture of cyanopeptides, and a total of 225 cyanopeptides were detected. Planktothrix sp. CPCC 735 produced the most (n = 68) cyanopeptides, and P. rubescens CPCC 732 produced the fewest (n = 27). Microcystins and anabaenopeptins were detected from all strains. Cyanopeptolins, microviridins and aeruginosins were detected from five, four and two strains, respectively. Cyanopeptolin (n = 80) and anabaenopeptin (n = 61) diversity was the greatest, whereas microcystins (n = 21) were the least diverse. Interestingly, three of the P. rubescens strains had different cyanopeptide profiles, despite being collected from the same lake at the same time. This study highlights the diversity of cyanopeptides produced by Planktothrix and further hints at the underestimated cyanopeptide diversity from subpopulations of chemotypic cyanobacteria in freshwater lakes.