Cyanobacterial extract as a source of nutrients for mixotrophic growth of Chlorella vulgaris and Nannochloropsis oculata

Rezasoltani, Samira; Vahabzadeh, Farzaneh; Shariatmadari, Zeinab; Ghanati, Faezeh

doi:10.1016/j.algal.2019.101480

Cited by 12 publications

(3 citation statements)

References 51 publications

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“…The synergistic effect of the cyanobacterial mixture could be mainly attributed to the exchange of metabolites such as hormones, vitamins, and enzymes. It has been reported that Anabaena and Nostoc strains are highly capable of producing biologically active growthstimulating phytohormones such as indole-3-acetic acid, and indole-3-propionic acid, brassinosteroid, and gibberellic acid, which would be a possible mechanism whereby the cyanobacteria exert beneficial stimulatory effects on their growth under the co-inoculation treatment strategy (Rezasoltani et al 2019, Shariatmadari et al 2015. The symbiotic interactions among cyanobacterial species have been previously reported to promote biocrust development.…”

Section: Resultsmentioning

confidence: 99%

Biostabilization of Gold Mine Tailings: Co-Inoculation of Cyanobacteria Under Sterile and Non-Sterile Conditions

Rezasoltani

Champagne

Mann

2021

Preprint

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Cyanobacterial crust formation has attracted attention for stabilizing erosion-susceptible soils in desert regions. However, limited information exists on its application in waste impoundments such as mine tailings. Identifying suitable inoculants with the ability to develop biocrusts in the more toxic conditions of mine tailings represents a challenge for exploiting this biotechnology for such applications. In this study, the performance of two nitrogen-fixing cyanobacteria (Anabaena sp. and Nostoc muscorum), individually and as a consortium, in creating biocrusts over gold mine tailings were investigated under sterile and non-sterile conditions. The results showed that Anabaena sp. and the co-inoculation of the species promoted higher synthesis of chlorophyll-a and total EPS compared to N. muscorum. The inoculated strains also exhibited different responses in the amount of the EPS fractions. The less soluble and more condensed tightly bound EPS represented a higher fraction of total EPS with co-inoculation and N. muscorum. With respect to wind erosion resistance and compressive strength of the biocrusts generated, co-inoculation showed better performance, followed by N. muscorum, while Anabaena sp. appeared to be less effective. The presence of indigenous microbial community within the tailings influenced the biostabilization performance of Anabaena sp., while the influence was insignificant under co-inoculation and N. muscorum. Overall, inoculating the cyanobacteria in a mixture with complementary traits (higher chlorophyll-a synthesis and total EPS secretion of Anabaena sp. vs. higher TB-EPS fraction and filamentous growth of N. muscorum) presented an effective strategy in the development of a resistant biocrust against wind erosion. With this inoculation strategy, the beneficial effects of the individual strains on biocrust formation could be combined, thus a comparatively stronger structure could be formed. Besides chlorophyll-a content, factors such as cyanobacteria morphology and EPS fractions would contribute to the biostabilization process. The results also suggested that sterilization of the tailings would influence the performance of cyanobacteria depending on the inoculant. Thus, the response of inoculants to other microbial communities should be considered prior to field-scale application.

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Section: Resultsmentioning

confidence: 99%

Biostabilization of Gold Mine Tailings: Co-Inoculation of Cyanobacteria Under Sterile and Non-Sterile Conditions

Rezasoltani

Champagne

Mann

2021

Preprint

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“…Increasing the biomass and lipid content of N. oculata was previously tested in the study of (Rezasoltani et al, 2019) with the addition of cyanobacteria Anabaena vaginicola extract. Cyanobacteria in the culture as a substitute for nitrogen fertilizer.…”

Section: Discussionmentioning

confidence: 99%

Polyploid Induction Enhance Pigment Production in Nannochoropsis oculata: A study using a mutagenesis agent.

Rahmawati

Yunianta

Risjani

2023

Preprint

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Directed mutagenesis by chemical induction using a mutagenesis agent to induce polyploidy on Nannochloropsis oculata can be an option to get higher biomass production of this microalgae. The aim of this research is to investigate the increase of biomass, pigment and DNA content of this microalgae after polyploid induction using a mutagenesis agent, colchicine. This microalgae induced with colchicine at a concentration of 0; 10; 100; 1,000 and 10,000 mg L-1 for 24, 48, and 72 hours for preliminary study. Further investigation using 48 hours treatment because it gives significant increase of biomass and pigment. The microalgae cultivated in sterilized seawater under constant light and aeration. A groups of 10 mg L-1 colchicine with 48 hour incubation showed significant increase of cell size and DNA content, but not in pigment. This groups might have an increase in another cell component, i.e. lipid. A groups of 1.000 mg L-1 colchicine induction for 48 hours, showed an increase in biomass and pigment production. So that, the use of 10 mg L-1 colchicine induction for 48 hours can increase the cell and genome size of N. oculata while a concentration of 1,000 mg L-1 did not increased genome size but increase the pigment and biomass production.

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“…A. vaginicola isolated from rice (Oryza, sativa L.) was obtained from the previous study and all the necessary methods used in this study were in accordance with the relevant guidelines [9]. BG11 medium which contains synthetic nitrogen and carbon sources and other inorganic salts (NaNO 3 and Na 2 CO 3 ), was used to support growth of the test cyanobacterium [10].…”

Section: Microbial Culture and Maintenancementioning

confidence: 99%

Lipids Productivity of Cyanobacterium Anabaena vaginicola in an Internally Illuminated Photobioreactor Using LED Bar Lights

Goldoost

Vahabzadeh

Fallah

2023

Preprint

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Concerns over environmental issues exists and desire to decrease of their extent, have directed efforts toward green energy production. Growth behavior of Anabaena vaginicola, was determined in a photobioreator which illuminated internally (IIPBR) using LED bar light. Excessive heat generated in the IIPBR was taken care of by applying a novel air-cooled system. Further note in experimentation was to find favorable cultivation conditions in the IIPBR for A. vaginicola growth and its lipids production capacity. The following results are expressed: 80 µmol photons m-2 s-1 as light intensity, 0.5 g/l as NaNO3, and 120 ml/min as CO2 amount being expressed in terms of aeration rate. The findings were interpreted in terms of a two-component system where the genes encoded to the relevant proteins are present in cyanobacteria and their expressiveness depends on environmental stress. By determining growth rate constant as 0.11 d-1, the productivity in terms of biomass formation was calculated as 202.6 mg L−1 d−1. While rate of lipids production by the test cyanobacterium is 15.65 mg L−1 d−1. Based on total energy used for IIPBR performance, biomass productivity per unit power input equals to 0.74 g W-1 d-1 and this is in favorable position compared with other photobioreactors.

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Cyanobacterial extract as a source of nutrients for mixotrophic growth of Chlorella vulgaris and Nannochloropsis oculata

Cited by 12 publications

References 51 publications

Biostabilization of Gold Mine Tailings: Co-Inoculation of Cyanobacteria Under Sterile and Non-Sterile Conditions

Biostabilization of Gold Mine Tailings: Co-Inoculation of Cyanobacteria Under Sterile and Non-Sterile Conditions

Polyploid Induction Enhance Pigment Production in Nannochoropsis oculata: A study using a mutagenesis agent.

Lipids Productivity of Cyanobacterium Anabaena vaginicola in an Internally Illuminated Photobioreactor Using LED Bar Lights

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