Cyanobacterial diversity held in microbial biological resource centers as a biotechnological asset: the case study of the newly established LEGE culture collection

Abstract

Cyanobacteria are a well-known source of bioproducts which renders culturable strains a valuable resource for biotechnology purposes. We describe here the establishment of a cyanobacterial culture collection (CC) and present the first version of the strain catalog and its online database (http://lege.ciimar.up.pt/). The LEGE CC holds 386 strains, mainly collected in coastal (48%), estuarine (11%), and fresh (34%) water bodies, for the most part from Portugal (84%). By following the most recent taxonomic classification, LEGE CC strains were classified into at least 46 genera from six orders (41% belong to the Synechococcales), several of them are unique among the phylogenetic diversity of the cyanobacteria. For all strains, primary data were obtained and secondary data were surveyed and reviewed, which can be reached through the strain sheets either in the catalog or in the online database. An overview on the notable biodiversity of LEGE CC strains is showcased, including a searchable phylogenetic tree and images for all strains. With this work, 80% of the LEGE CC strains have now their 16S rRNA gene sequences deposited in GenBank. Also, based in primary data, it is demonstrated that several LEGE CC strains are a promising source of extracellular polymeric substances (EPS). Through a review of previously published data, it is exposed that LEGE CC strains have the potential or actual capacity to produce a variety of biotechnologically interesting compounds, including common cyanotoxins or unprecedented bioactive molecules. Phylogenetic diversity of LEGE CC strains does not entirely reflect chemodiversity. Further bioprospecting should, therefore, account for strain specificity of the valuable cyanobacterial holdings of LEGE CC.Electronic supplementary materialThe online version of this article (10.1007/s10811-017-1369-y) contains supplementary material, which is available to authorized users.

Biofilm formation behaviour of marine filamentous cyanobacterial strains in controlled hydrodynamic conditions

“…In order to evaluate the behaviour of different filamentous cyanobacteria from different marine environments, three cyanobacterial strains were used in this study. All strains were obtained from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE‐CC) located at CIIMAR, Portugal (Ramos et al ., ). Nodosilinea sp.…”

BrtB is an O-alkylating enzyme that generates fatty acid-bartoloside esters

“…The cyanobacterium Synechocystis salina LEGE 06099 was obtained from the LEGEcc 30 . Cultures were grown in Z8 medium supplemented with 25 g L −1 sea salt (Tropic Marin), at 25°C, under a 14:10 h light/dark cycle and constant aeration 14 .…”

“…A range of 6-heptynoic acid (2) concentrations (corresponding to 5, 25, 50, 100, 175, 250, 500, 750, and 1000 μM and prepared from a 100 mM stock solution) were used to determine the initial reaction rates. Reactions were incubated at 37°C and aliquots (20 μL) were collected at different time points (5,10,20,30, and 60 min) and quenched with 60 μL of a cold mixture of MeOH/ MeCN (1:1, v/v), vortexed immediately and incubated in ice for 10 min. Quenched reactions were then centrifuged at 17 000 × g, 4°C, 10 min) and analyzed by LC-HRESIMS/MS.…”

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

“…The three microalgae species used in this work were obtained from Blue Biotechnology and Ecotoxicology Culture Collection (LEGE-CC) [57]. The microalgae were: Parachlorella kessleri (LEGE Z-001), Microcystis aeruginosa (LEGE 91094), and Chrysosporum ovalisporum (LEGE X-001).…”

“…M. aeruginosa is characterized by small cells (2.6 ± 0.3 µm diameter) and is unicellular whereas C. ovalisporum is a filamentous species, with trichomes varying from 0.5-1.0 mm in lenght, narrowing in the extremities, and cells with 4-5 µm diameter (Figure 8a,b, respectively). The strains used are known to produce, respectively, the toxins MC-LR and CYN [57][58][59][60]. Furthermore P. kesseleri is a unicellular alga.…”