Cyanobacterial cellulose synthesis in the light of the photanol concept

Schuurmans, R. Milou; Matthijs, H.C.P.; Stal, Lucas J.; Hellingwerf, Klaas J.

doi:10.1002/9781118402238.ch11

Cited by 4 publications

(3 citation statements)

References 66 publications

(89 reference statements)

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“…Despite the ubiquitous distribution of Alg8/BcsA, no sequences possessing the multi-domain architecture of Alg44 were identified, whereas 70 out of 454 possess both the Glyco_tranf_2_3 and PilZ domains typical of the cellulose synthase. Our results corroborate and expand previous data suggesting that cellulose synthase are widespread among cyanobacteria and that cellulose biosynthesis is a common feature in these organisms 46 47 . Considering the absence of the identified proteins involved in alginate or cellulose modification and export in the majority of the cyanobacteria, it is likely that their synthases interact with different cell components or that the proteins are different than those of other bacteria.…”

Section: Discussionsupporting

confidence: 92%

Phylum-wide analysis of genes/proteins related to the last steps of assembly and export of extracellular polymeric substances (EPS) in cyanobacteria

Pereira

Mota

Vieira

et al. 2015

Sci Rep

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Many cyanobacteria produce extracellular polymeric substances (EPS) with particular characteristics (e.g. anionic nature and presence of sulfate) that make them suitable for industrial processes such as bioremediation of heavy metals or thickening, suspending or emulsifying agents. Nevertheless, their biosynthetic pathway(s) are still largely unknown, limiting their utilization. In this work, a phylum-wide analysis of genes/proteins putatively involved in the assembly and export of EPS in cyanobacteria was performed. Our results demonstrated that most strains harbor genes encoding proteins related to the three main pathways: Wzy-, ABC transporter-, and Synthase-dependent, but often not the complete set defining one pathway. Multiple gene copies are mainly correlated to larger genomes, and the strains with reduced genomes (e.g. the clade of marine unicellular Synechococcus and Prochlorococcus), seem to have lost most of the EPS-related genes. Overall, the distribution of the different genes/proteins within the cyanobacteria phylum raises the hypothesis that cyanobacterial EPS production may not strictly follow one of the pathways previously characterized. Moreover, for the proteins involved in EPS polymerization, amino acid patterns were defined and validated constituting a novel and robust tool to identify proteins with similar functions and giving a first insight to which polymer biosynthesis they are related to.

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Section: Discussionsupporting

confidence: 92%

Phylum-wide analysis of genes/proteins related to the last steps of assembly and export of extracellular polymeric substances (EPS) in cyanobacteria

Pereira

Mota

Vieira

et al. 2015

Sci Rep

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“…Furthermore, a Tol-C like protein encoded by tlr1605 was also essential for successful cellulose production. The authors propose tripartite secretion system for cellulose production mechanism in this cyanobacterium [ 112 , 113 ]. The level of cellulose production in cyanobacteria is relatively low and not sufficient for commercial use [ 110 ].…”

Section: Eps Produced By Cyanobacteriamentioning

confidence: 99%

Modifying the Cyanobacterial Metabolism as a Key to Efficient Biopolymer Production in Photosynthetic Microorganisms

Ciebiada

Kubiak

Daroch

2020

IJMS

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Cyanobacteria are photoautotrophic bacteria commonly found in the natural environment. Due to the ecological benefits associated with the assimilation of carbon dioxide from the atmosphere and utilization of light energy, they are attractive hosts in a growing number of biotechnological processes. Biopolymer production is arguably one of the most critical areas where the transition from fossil-derived chemistry to renewable chemistry is needed. Cyanobacteria can produce several polymeric compounds with high applicability such as glycogen, polyhydroxyalkanoates, or extracellular polymeric substances. These important biopolymers are synthesized using precursors derived from central carbon metabolism, including the tricarboxylic acid cycle. Due to their unique metabolic properties, i.e., light harvesting and carbon fixation, the molecular and genetic aspects of polymer biosynthesis and their relationship with central carbon metabolism are somehow different from those found in heterotrophic microorganisms. A greater understanding of the processes involved in cyanobacterial metabolism is still required to produce these molecules more efficiently. This review presents the current state of the art in the engineering of cyanobacterial metabolism for the efficient production of these biopolymers.

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“…Algal cellulose has been analyzed with regard to the crystalline features such as allomorphism ( Park et al, 2015 ). In similar lines, the cellulosic algae proved useful for freeze-fracture studies where putative synthase complexes were first visualized in the plasma membrane, but without much success on the biochemical and molecular mechanisms ( Schuurmans et al, 2014 ). The chordate Dictyostelium discoideum synthesizes cellulose at different stages in the life cycle, thus outlining defined cellulose synthase activity, as well as sophisticated genetic approaches for cellulose biosynthesis studies ( Hobdey et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Biochemistry, Synthesis, and Applications of Bacterial Cellulose: A Review

Mishra

Singh

Pattnaik

et al. 2022

Front. Bioeng. Biotechnol.

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The potential of cellulose nanocomposites in the new-generation super-performing nanomaterials is huge, primarily in medical and environment sectors, and secondarily in food, paper, and cosmetic sectors. Despite substantial illumination on the molecular aspects of cellulose synthesis, various process features, namely, cellular export of the nascent polysaccharide chain and arrangement of cellulose fibrils into a quasi-crystalline configuration, remain obscure. To unleash its full potential, current knowledge on nanocellulose dispersion and disintegration of the fibrillar network and the organic/polymer chemistry needs expansion. Bacterial cellulose biosynthesis mechanism for scaled-up production, namely, the kinetics, pathogenicity, production cost, and product quality/consistency remain poorly understood. The bottom-up bacterial cellulose synthesis approach makes it an interesting area for still wider and promising high-end applications, primarily due to the nanosynthesis mechanism involved and the purity of the cellulose. This study attempts to identify the knowledge gap and potential wider applications of bacterial cellulose and bacterial nanocellulose. This review also highlights the manufacture of bacterial cellulose through low-cost substrates, that is, mainly waste from brewing, agriculture, food, and sugar industries as well as textile, lignocellulosic biorefineries, and pulp mills.

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Cyanobacterial cellulose synthesis in the light of the photanol concept

Cited by 4 publications

References 66 publications

Phylum-wide analysis of genes/proteins related to the last steps of assembly and export of extracellular polymeric substances (EPS) in cyanobacteria

Phylum-wide analysis of genes/proteins related to the last steps of assembly and export of extracellular polymeric substances (EPS) in cyanobacteria

Modifying the Cyanobacterial Metabolism as a Key to Efficient Biopolymer Production in Photosynthetic Microorganisms

Biochemistry, Synthesis, and Applications of Bacterial Cellulose: A Review

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