Cyanobacteria in hot pursuit: Characterization of cyanobacteria strains, including novel taxa, isolated from geothermal habitats from different ecoregions of the world

Jasser, Iwona; Panou, Manthos; Khomutovska, Nataliia; Sandzewicz, Małgorzata; Panteris, Emmanuel; Niyatbekov, Toirbek; Łach, Łukasz; Kwiatowski, Jan; Kokociński, Mikołaj; Gkelis, Spyros

doi:10.1016/j.ympev.2022.107454

Cited by 14 publications

(17 citation statements)

References 85 publications

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“…In the Non-layered type mat sample (E15), growing in a hot spring along Gant River, a new filamentous genus, and species of cyanobacteria, Hillbrichtia pamiri a gen. et sp. nov., was found to dominate ( Jasser et al, 2022 ). However, coccal Chlorogloea and Aphanocapsa genera, as well as heterocystous Calothrix, were also numerous in the Non-layered type mats.…”

Section: Resultsmentioning

confidence: 99%

“…However, in the Pamirian hot spring (E15), with temperature around 50°C, a filamentous H. pamiria gen. Nov. sp. nov. belonging to the subclass Oscillatoriophycidae ( Jasser et al, 2022 ), was the most abundant cyanobacterium. When it comes to the psychrophilic mats, Oscillatoriales and Nostocales are the most common cyanobacterial orders, according to the literature ( Taton et al, 2003 ; Prieto-Barajas et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…High water temperature also requires various physiological adaptations, such as producing antioxidants, or inducing various defense genes, for example genes responsible for biosynthesis of heat shock proteins or desaturases, enzymes responsible for fatty acids desaturations in membranes ( Ismaiel and Piercey-Normore, 2021 ). Not only induction of certain genes but also their number, as in the case of HSPs genes, is connected with the ability of cyanobacterial species to grow in stressful conditions, for example high temperature environments ( Jasser et al, 2022 ). In this way we can say that salinity or temperature may influence the composition and structure of cyanobacterial communities by favoring the growth of organisms that can thrive in such conditions.…”

Section: Discussionmentioning

confidence: 99%

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Salinity matters the most: How environmental factors shape the diversity and structure of cyanobacterial mat communities in high altitude arid ecosystems

et al. 2023

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IntroductionMicrobial mats are complex communities of benthic microorganisms that occur at the soil-water interphase in lakes’ shores, streams, and ponds. In the cold, mountainous desert of Eastern Pamir (Tajikistan), where scarce water bodies are influenced by extreme environmental conditions, photosynthetic cyanobacteria form diverse mats. The mats are characterized by different morphology and thickness. Their habitats exhibit a wide range of conditions; from oligosaline to hypersaline, oligotrophic to hypertrophic, and from cold ponds to hot springs. The aim of the present study was to reveal the taxonomic composition and structure of these mats and to examine which environmental factors influence them.MethodsFifty-one mats were collected from small water bodies around Bulunkul, Karakul, and Rangkul Lakes in 2015 and 2017. The physical and chemical properties of the water were measured in situ, while the concentration of nutrients was analyzed ex-situ. To reveal the taxonomic composition of the mats, the hypervariable V3-V4 region of the 16S rRNA gene was examined using NGS technology.ResultsThe results of bioinformatic analyses were compared with microscopic observations. They showed that Cyanobacteria was the dominant phylum, constituting on average 35% of bacterial ASVs, followed by Proteobacteria (28%), Bacteroidota (11%), and Firmicutes (9%). Synechococcales, Oscillatoriales, and Nostocales orders prevailed in Oxyphotobacteria, with a low contribution of Chroococcales, Gloeobacterales, and Chroococcidiopsidales. Occasionally the non-photosynthetic Vampirivibrionia (Melainabacteria) and Sericytochromatia from sister clades to Oxyphotobacteria were noted in the samples. Moreover, there was a high percentage of unidentified cyanobacterial sequences, as well as the recently described Hillbrichtia pamiria gen. et sp. nov., present in one of the samples. Salinity, followed by Na and K concentrations, correlated positively with the composition and structure of Oxyphotobacteria on different taxonomic levels and the abundance of all bacterial ASVs.DiscussionThe study suggests that the investigated communities possibly host more novel and endemic species. Among the environmental factors, salinity influenced the Oxyphotobacteria communities the most. Overall, the microenvironmental factors, i.e. the conditions in each of the reservoirs seemed to have a larger impact on the diversity of microbial mats in Pamir than the “subregional” factors, related to altitude, mean annual air temperature and distance between these subregions.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Salinity matters the most: How environmental factors shape the diversity and structure of cyanobacterial mat communities in high altitude arid ecosystems

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“…Characterization of thermophilic cyanobacteria has been extensively performed in alkaline siliceous hot springs of Yellow National Park (YNP), United States, particularly the thick mats of Octopus and Mushroom springs (Kees et al, 2022). Members belonging to the genera Leptolyngbya, Fischerella, Mastigocladus, Synechococcuslike, Geomargarita, Gloeocapsa, Gloeocapsopsis, Oscillatoria, and Thermosynnechococcus are predominant cyanobacteria occurring in hot springs spanning various ecoregions of the world (Amarouche-Yala et al, 2014;Alcamán et al, 2017;Jasser et al, 2022). A thermophilic cyanobacterium, Mastigocladus laminosus UU774, isolated from a hot spring, Taptapani, in India (19.3011 • N, 84.2424 • E) is discussed in the present study.…”

Section: Introductionmentioning

confidence: 99%

Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement

et al. 2022

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The present study focuses on the stress response of a filamentous, AT-rich, heterocystous cyanobacterium Mastigocladus laminosus UU774, isolated from a hot spring, Taptapani, located in the eastern part of India. The genome of UU774 contains an indispensable fragment, scaffold_38, of unknown origin that is implicated during severe nitrogen and nutrition stress. Prolonged exposure to nitrogen compounds during starvation has profound adverse effects on UU774, leading to loss of mobility, loss of ability to fight pathogens, reduced cell division, decreased nitrogen-fixing ability, reduced ability to form biofilms, reduced photosynthetic and light-sensing ability, and reduced production of secreted effectors and chromosomal toxin genes, among others. Among genes showing extreme downregulation when grown in a medium supplemented with nitrogen with the fold change > 5 are transcriptional regulator gene WalR, carbonic anhydrases, RNA Polymerase Sigma F factor, fimbrial protein, and twitching mobility protein. The reduced expression of key enzymes involved in the uptake of phosphate and enzymes protecting oxygen-sensitive nitrogenases is significant during the presence of nitrogen. UU774 is presumed to withstand heat by overexpressing peptidases that may be degrading abnormally folded proteins produced during heat. The absence of a key gene responsible for heterocyst pattern formation, patS, and an aberrant hetN without a functional motif probably lead to the formation of a chaotic heterocyst pattern in UU774. We suggest that UU774 has diverged from Fischerella sp. PCC 9339, another hot spring species isolated in the United States.

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“…For example, microalgae could be useful in aquaculture, producing foods, feeds, biofuels, fertilizers, nutraceuticals, secondary metabolites, pigments, and a myriad of bioactive biochemicals ( Singh et al, 2005 ; Udayan et al, 2017 ; Cobos et al, 2020 ). In addition, cyanobacteria are isolable and cultivable in laboratory conditions ( Ernst, 1991 ; Badr et al, 2018 ; Prihantini, 2020 ; Thilak et al, 2020 ; Jasser et al, 2022 ; Singh and Kumar, 2022 ). Moreover, cyanobacteria have a relatively short doubling time and can produce high biomass volumes ( Yu et al, 2015 ; Jaiswal et al, 2018 ; Mastropetros et al, 2022 ; Prabha et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Genomic analysis and biochemical profiling of an unaxenic strain of Synechococcus sp. isolated from the Peruvian Amazon Basin region

et al. 2022

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Cyanobacteria are diverse photosynthetic microorganisms able to produce a myriad of bioactive chemicals. To make possible the rational exploitation of these microorganisms, it is fundamental to know their metabolic capabilities and to have genomic resources. In this context, the main objective of this research was to determine the genome features and the biochemical profile of Synechococcus sp. UCP002. The cyanobacterium was isolated from the Peruvian Amazon Basin region and cultured in BG-11 medium. Growth parameters, genome features, and the biochemical profile of the cyanobacterium were determined using standardized methods. Synechococcus sp. UCP002 had a specific growth rate of 0.086 ± 0.008 μ and a doubling time of 8.08 ± 0.78 h. The complete genome of Synechococcus sp. UCP002 had a size of ∼3.53 Mb with a high coverage (∼200x), and its quality parameters were acceptable (completeness = 99.29%, complete and single-copy genes = 97.5%, and contamination = 0.35%). Additionally, the cyanobacterium had six plasmids ranging from 24 to 200 kbp. The annotated genome revealed ∼3,422 genes, ∼ 3,374 protein-coding genes (with ∼41.31% hypothetical protein-coding genes), two CRISPR Cas systems, and 61 non-coding RNAs. Both the genome and plasmids had the genes for prokaryotic defense systems. Additionally, the genome had genes coding the transcription factors of the metalloregulator ArsR/SmtB family, involved in sensing heavy metal pollution. The biochemical profile showed primary nutrients, essential amino acids, some essential fatty acids, pigments (e.g., all-trans-β-carotene, chlorophyll a, and phycocyanin), and phenolic compounds. In conclusion, Synechococcus sp. UCP002 shows biotechnological potential to produce human and animal nutrients and raw materials for biofuels and could be a new source of genes for synthetic biological applications.

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Cyanobacteria in hot pursuit: Characterization of cyanobacteria strains, including novel taxa, isolated from geothermal habitats from different ecoregions of the world

Cited by 14 publications

References 85 publications

Salinity matters the most: How environmental factors shape the diversity and structure of cyanobacterial mat communities in high altitude arid ecosystems

Salinity matters the most: How environmental factors shape the diversity and structure of cyanobacterial mat communities in high altitude arid ecosystems

Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement

Genomic analysis and biochemical profiling of an unaxenic strain of Synechococcus sp. isolated from the Peruvian Amazon Basin region

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