Cyanide-resistant respiration in yeast

Pj, Ainsworth; Ball, AJ; Er, Tustanoff

doi:10.1016/0003-9861(80)90420-8

Cited by 21 publications

(1 citation statement)

References 30 publications

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“…2). The residual NADH-oxidizing activity in mitochondrial preparations of the mutant still exhibited respiratory control (Table III), indicating that this activity was not entirely due to contamination with nonrespiratory chain-linked oxidases (42).…”

Section: Generation Of Ethanol-free Nadh In Oxygen Uptakementioning

confidence: 95%

The Saccharomyces cerevisiae NDE1 and NDE2 Genes Encode Separate Mitochondrial NADH Dehydrogenases Catalyzing the Oxidation of Cytosolic NADH

Luttik¹,

Overkamp²,

Kötter³

et al. 1998

Journal of Biological Chemistry

276

279

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In Saccharomyces cerevisiae, the NDI1 gene encodes a mitochondrial NADH dehydrogenase, the catalytic side of which projects to the matrix side of the inner mitochondrial membrane. In addition to this NADH dehydrogenase, S. cerevisiae exhibits another mitochondrial NADH-dehydrogenase activity, which oxidizes NADH at the cytosolic side of the inner membrane. To investigate whether open reading frames YMR145c/NDE1 and YDL 085w/NDE2, which exhibit sequence similarity with NDI1, encode the latter enzyme, NADH-dependent mitochondrial respiration was assayed in wild-type S. cerevisiae and nde deletion mutants. Mitochondria were isolated from aerobic, glucose-limited chemostat cultures grown at a dilution rate (D) of 0.10 h ؊1 , in which reoxidation of cytosolic NADH by wild-type cells occurred exclusively by respiration. Compared with the wild type, rates of mitochondrial NADH oxidation were about 3-fold reduced in an nde1⌬ mutant and unaffected in an nde2⌬ mutant. NADH-dependent mitochondrial respiration was completely abolished in an nde1⌬ nde2⌬ double mutant. Mitochondrial respiration of substrates other than NADH was not affected in nde mutants. In shake flasks, an nde1⌬ nde2⌬ mutant exhibited reduced specific growth rates on ethanol and galactose but not on glucose. Glucose metabolism in aerobic, glucose-limited chemostat cultures (D ‫؍‬ 0.10 h ؊1 ) of an nde1⌬ nde2⌬ mutant was essentially respiratory. Apparently, under these conditions alternative systems for reoxidation of cytosolic NADH could replace the role of Nde1p and Nde2p in S. cerevisiae.

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Section: Generation Of Ethanol-free Nadh In Oxygen Uptakementioning

confidence: 95%

The Saccharomyces cerevisiae NDE1 and NDE2 Genes Encode Separate Mitochondrial NADH Dehydrogenases Catalyzing the Oxidation of Cytosolic NADH

Luttik¹,

Overkamp²,

Kötter³

et al. 1998

Journal of Biological Chemistry

276

279

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Evidence of Antimycin-Insensitive Respiration in a Commercial Brewing Yeast

Lodolo¹,

O'Connor-Cox²,

Axcell³

1999

Journal of the Institute of Brewing

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Petite mutants of sophorolipid‐producing Candida yeasts

Hommel¹,

Baum²,

Kleber³

1994

J. Basic Microbiol.

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Treatment of both Candida apicola IMET 43747 and Candida bombicola ATCC 22214 with N-methyl-N'-nitro-N-nitroso guanidine resulted in formation of auxotrophic mutants and cytochrome oxidase negative mutants. The deficiency of intact cytochrome oxidase did not affect the ability to produce sophorose lipid. This indicates that intact mitochondrial energy supplying system is not necessary for the production of the extracellular sophorose lipids by both Candida yeasts.

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Cyanide-resistant respiration in yeast

Cited by 21 publications

References 30 publications

The Saccharomyces cerevisiae NDE1 and NDE2 Genes Encode Separate Mitochondrial NADH Dehydrogenases Catalyzing the Oxidation of Cytosolic NADH

The Saccharomyces cerevisiae NDE1 and NDE2 Genes Encode Separate Mitochondrial NADH Dehydrogenases Catalyzing the Oxidation of Cytosolic NADH

Evidence of Antimycin-Insensitive Respiration in a Commercial Brewing Yeast

Petite mutants of sophorolipid‐producing Candida yeasts

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