CXCR5 Knockdown Attenuates Hippocampal Neurogenesis Deficits and Cognitive Impairment in a Mouse Model of Sepsis-associated Encephalopathy

Shen, Yanan; Jing, Ling; Zhang, Yuan; Bao, Huihui; Vohra, Akbar; Si, Yanna; Pan, Cailong; Xing, Yan

doi:10.1016/j.neuroscience.2020.03.013

Cited by 13 publications

(18 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CXCL13 and CXCR5 are involved in neurodegeneration and cognitive decline [ 9 ], which are related to a deficit in hippocampal neurogenesis [ 38 , 39 ]. In previous work, we showed that CXCR5 may inhibit proliferation, differentiation, and survival of hippocampal neuronal stem cells in an animal model of sepsis, leading to learning and memory impairments [ 14 ]. Hippocampal CXCR5 upregulation causes sevoflurane anesthesia-induced cognitive dysfunction via PI3K/Akt signaling [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…The shRNAs (5 μg) were dissolved in 5 μL RNase-free water, mixed with 5 μL transfection reagent (Entranster™-in vivo, Engreen Biosystem, Beijing, China), and injected i.c.v. at 3 days before exploratory laparotomy as described previously [ 14 ]. CXCL13 knockdown was verified using Western blot analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The only known CXCL13 receptor is C-X-C motif chemokine receptor 5 (CXCR5), which is highly expressed on B lymphocytes and certain T cells in cerebrospinal fluid, and which helps guide lymphocyte tracking within secondary lymphoid tissues [ 10 – 13 ]. Our previous work showed that CXCR5 contributes to learning and memory impairment in an animal model of sepsis [ 14 ]. CXCR5 is expressed in neuronal precursor cells of the brain, which migrate across brain endothelial cells upon exposure to CXCL13 [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated mice

Shen

Zhang

Chen

et al. 2020

J Neuroinflammation

Self Cite

View full text Add to dashboard Cite

Background Perioperative neurocognitive disorders (PNDs) occur frequently after surgery and worsen patient outcome. How C-X-C motif chemokine (CXCL) 13 and its sole receptor CXCR5 contribute to PNDs remains poorly understood. Methods A PND model was created in adult male C57BL/6J and CXCR5−/− mice by exploratory laparotomy. Mice were pretreated via intracerebroventricular injection with recombinant CXCL13, short hairpin RNA against CXCL13 or a scrambled control RNA, or ERK inhibitor PD98059. Then surgery was performed to induce PNDs, and animals were assessed in the Barnes maze trial followed by a fear-conditioning test. Expression of CXCL13, CXCR5, and ERK in hippocampus was examined using Western blot, quantitative PCR, and immunohistochemistry. Levels of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) in hippocampus were assessed by Western blot. Results Surgery impaired learning and memory, and it increased expression of CXCL13 and CXCR5 in the hippocampus. CXCL13 knockdown partially reversed the effects of surgery on CXCR5 and cognitive dysfunction. CXCR5 knockout led to similar cognitive outcomes as CXCL13 knockdown, and it repressed surgery-induced activation of ERK and production of IL-1β and TNF-α in hippocampus. Recombinant CXCL13 induced cognitive deficits and increased the expression of phospho-ERK as well as IL-1β and TNF-α in hippocampus of wild-type mice, but not CXCR5−/− mice. PD98059 partially blocked CXCL13-induced cognitive dysfunction as well as production of IL-1β and TNF-α. Conclusions CXCL13-induced activation of CXCR5 may contribute to PNDs by triggering ERK-mediated production of pro-inflammatory cytokines in hippocampus.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated mice

Shen

Zhang

Chen

et al. 2020

J Neuroinflammation

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the other hand, the modication of methods comprises the development of processes by using alternative energy resources or improvement of yield with synergistic effects such as mixing program, 22 ultrasonic irradiation, 47,62 microwave technology, [81][82][83][84][85][86][87] high temperature, 88 and hydrodynamic cavitation. 89 Our group has studied amphiphilic phosphotungstatepaired ionic copolymer 90 and molybdenum-based catalysts [91][92][93][94][95][96][97][98] on various supports for the epoxidation of different alkenes and good results were obtained. Moreover, recent developments in the application of molybdenum-based catalysts for epoxidation have been reviewed as well.…”

Section: Introductionmentioning

confidence: 99%

Catalytic developments in the epoxidation of vegetable oils and the analysis methods of epoxidized products

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The catalysts reusability study and the ICP‐OES analysis showed that there is no considerable loss of its activity and Mo species content after six cycles. Shen et al . reported a selective etching strategy to synthesize highly dispersed Mo incorporated hollow mesoporous silica catalyst (Mo/HMSS, Table S5, #30‐36).…”

Section: Mo‐based Catalystsmentioning

confidence: 99%

Recent Advances in Facile Liquid Phase Epoxidation of Light Olefins over Heterogeneous Molybdenum Catalysts

Yan

Liu

Wang

et al. 2019

The Chemical Record

View full text Add to dashboard Cite

Molybdenum complexes are versatile and efficient for liquid phase olefin epoxidation reactions. Rational design of catalysts is critical to achieve high atom efficiency during epoxidation processes. Although liquid phase epoxidation has been a popular topic for decades, three key issues, (a) rational control of morphology of molybdenum nanoparticles, (b) manipulating metal‐support interaction and (c) altering electronic configuration at molybdenum center remains unsolved in this area. Therefore, in this paper, we have critically revised recent research progress on heterogeneous molybdenum catalysts for facile liquid phase olefin epoxidation in terms of catalyst synthesis, surface characterization, catalytic performance and structure‐function relationship. Furthermore, plausible reaction mechanisms will be systematically discussed with the aim to provide insights into fundamental understanding on novel epoxidation chemistry.

show abstract

CXCR5 Knockdown Attenuates Hippocampal Neurogenesis Deficits and Cognitive Impairment in a Mouse Model of Sepsis-associated Encephalopathy

Cited by 13 publications

References 34 publications

Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated mice

Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated mice

Catalytic developments in the epoxidation of vegetable oils and the analysis methods of epoxidized products

Recent Advances in Facile Liquid Phase Epoxidation of Light Olefins over Heterogeneous Molybdenum Catalysts

Contact Info

Product

Resources

About