CXCL5 Downregulation in Villous Tissue Is Correlated With Recurrent Spontaneous Abortion

Zhang, Sainan; Ding, Jinli; Wang, Jiayu; Yin, Tailang; Zhang, Yan; Yang, Jing

doi:10.3389/fimmu.2021.717483

Cited by 10 publications

(10 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study indicated that CXCL1 is necessary for decidual angiogenesis during the first trimester 30 . CXCL5 was also demonstrated to promote trophoblast migration, invasion, and epithelial‐to‐mesenchymal transition through PI3K/AKT/ERK1/2 signaling pathway 31 . Similarly, CXCL8 (interleukin 8) was demonstrated to directly increase the proliferation, migration, and invasion of EVT in concomitant with promoted production of MMP2 and MMP9 32 .…”

Section: Discussionmentioning

confidence: 98%

Spheroid formation induces chemokine production in trophoblast‐derived Swan71 cells

Kanda

Kagami

Iizuka

et al. 2023

American J Rep Immunol

View full text Add to dashboard Cite

Problem: In the cell column of anchoring villi, the cytotrophoblast differentiates into extravillous trophoblast (EVT) and invades the endometrium in contact with maternal immune cells. Recently, chemokines were proposed to regulate the decidual immune response. To investigate the roles of chemokines around the anchoring villi, we examined the expression profiles of chemokines in the first-trimester trophoblast-derived Swan71 cells using a three-dimensional culture model. Method of Study:The gene expressions in the spheroid-formed Swan71 cells were examined by microarray and qPCR analyses. The protein expressions were examined by immunochemical staining. The chemoattractant effects of spheroid-formed Swan71 cells were examined by migration assay using monocyte-derived THP-1 cells. Results:The expressions of an EVT marker, laeverin, and matrix metalloproteases, MMP2 and MMP9, were increased in the spheroid-cultured Swan71 cells. Microarray and qPCR analysis revealed that mRNA expressions of various chemokines, CCL2, CCL7, CCL20, CXCL1, CXCL2, CXCL5, CXCL6, CXCL8, and CXCL10, in the spheroid-cultured Swan71 cells were up-regulated as compared with those in the monolayer-cultured Swan71 cells. These expressions were significantly suppressed by hypoxia. Migration assay showed that culture media derived from the spheroid-formed Swan71 cells promoted THP-1 cell migration. Conclusion:This study indicated that chemokine expressions in Swan71 cells increase under a spheroid-forming culture and the culture media have chemoattractant effects.Since three-dimensional cell assembling in the spheroid resembles the structure of the cell column, this study also suggests that chemokines play important roles in the interaction between EVT and immune cells in their early differentiation stage.

show abstract

Section: Discussionmentioning

confidence: 98%

Spheroid formation induces chemokine production in trophoblast‐derived Swan71 cells

Kanda

Kagami

Iizuka

et al. 2023

American J Rep Immunol

View full text Add to dashboard Cite

show abstract

“…A growing number of studies have shown that EMT plays an important role in embryo formation. For example, EMT has been shown to regulate embryonic stem cell differentiation, and the induction of EMT enhanced trophoblast cell invasion and migration [ 34 , 35 ]. Our results here revealed that IL-23 treatment upregulated E-cadherin expression but downregulated N-cadherin expression, implying the inhibitory effects of IL-23 treatment on EMT in trophoblast cells.…”

Section: Discussionmentioning

confidence: 99%

IL-23 Inhibits Trophoblast Proliferation, Migration, and EMT via Activating p38 MAPK Signaling Pathway to Promote Recurrent Spontaneous Abortion

Ning

et al. 2022

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

Recurrent spontaneous abortion (RSA), defined as the loss of ≥2 consecutive pregnancies before the 24th gestational week, affects 1%-2% women during their reproductive age [1]. As a common condition in reproductive medicine, RSA is reported in 1-2% of fertile couples seeking pregnancy and inflicts a significant physical, emotional, and financial burden on many families [2]. Many factors are thought to contribute to RSA, such as parental chromosomal abnormalities, maternal thrombophilia, immune dysfunction, and various endocrine disorders [3]. In addition, the age of the pregnant mother has also been reported to be a strong independent risk factor for miscarriage, and in particular, the risk of fetal miscarriage greatly increases after 35 years of age [4]. However, in many cases, the causes of RSA could not be easily identified, while the evidence-based diagnostic and treatment strategies are in short supply and the prospects of treatment are not as promising as they could be [5].Interleukin 23 (IL-23), which is composed of a p40 subunit and a specific p19 subunit, belongs to the IL-12 cytokine family and is secreted by dendritic cells and macrophages [6,7]. Accumulated reports have confirmed that IL-23 participates in the development of some autoimmune inflammatory diseases, including psoriasis, arthritis, and inflammatory bowel disease, thus serving as a critical therapeutic target for the improvement of inflammatory diseases [8][9][10]. In addition, another study reported that IL-23 was upregulated in patients with RSA [11]. Moreover, both IL-17 and IL-23 were verified to inhibit Langerhans cell (LC) migration in a psoriasis As a vital problem in reproductive health, recurrent spontaneous abortion (RSA) affects about 1% of women. We performed this study with an aim to explore the molecular mechanism of interleukin-23 (IL-23) and find optimal or effective methods to improve RSA. First, ELISA was applied to evaluate the expressions of IL-23 and its receptor in HTR-8/SVneo cells after IL-23 treatment. CCK-8, TUNEL, wound healing and transwell assays were employed to assess the proliferation, apoptosis, migration and invasion of HTR-8/SVneo cells, respectively. Additionally, the expressions of apoptosis-, migration-, epithelial-mesenchymal transition-(EMT-) and p38 MAPK signaling pathway-related proteins were measured by western blotting. To further investigate the relationship between IL-23 and p38 MAPK signaling pathway, HTR-8/SVneo cells were treated for 1 h with p38 MAPK inhibitor SB239063, followed by a series of cellular experiments on proliferation, apoptosis, migration and invasion, as aforementioned. The results showed that IL-23 and its receptors were greatly elevated in IL-23-treated HTR-8/SVneo cells. Additionally, IL-23 demonstrated suppressive effects on the proliferation, apoptosis, migration, invasion and EMT of IL-23-treated HTR-8/SVneo cells. More importantly, the molecular mechanism of IL-23 was revealed in this study; that is to say, IL-23 inhibited the proliferation, apoptosis, migration, invas...

show abstract

“…LTA (lymphotoxin alpha) [52], CXCL5 [53], MALAT1 [54] and TMEM70 [55] have been demonstrated to function in hypertension, but these genes might be novel targets for GDM. CXCL5 has been shown to be activated abortion [56].…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes and signaling pathways in gestational diabetes mellitus by integrated bioinformatics analysis

Vastrad¹,

Vastrad²

2021

Preprint

View full text Add to dashboard Cite

Gestational diabetes mellitus (GDM) is a metabolic disorder during pregnancy. Numerous biomarkers have been identified that are linked with the occurrence and development of GDM. The aim of this investigation was to identify differentially expressed genes (DEGs) in GDM using a bioinformatics approach to elucidate their molecular pathogenesis. GDM associated expression profiling by high throughput sequencing dataset (GSE154377) was obtained from Gene Expression Omnibus (GEO) database including 28 normal pregnancy samples and 33 GDM samples. DEGs were identified using DESeq2. The gene ontology (GO) and REACTOME pathway enrichments of DEGs were performed by g:Profiler. Protein-protein interaction (PPI) networks were assembled with Cytoscape software and separated into modules using the PEWCC1 algorithm. MiRNA-hub gene regulatory network and TF-hub gene regulatory network were performed with the miRNet database and NetworkAnalyst database. Receiver Operating Characteristic (ROC) analyses was conducted to validate the hub genes. A total of 953 DEGs were identified, of which 478 DEGs were up regulated and 475 DEGs were down regulated. Furthermore, GO and REACTOME pathway enrichment analysis demonstrated that these DEGs were mainly enriched in multicellular organismal process, cell activation, formation of the cornified envelope and hemostasis. TRIM54, ELAVL2, PTN, KIT, BMPR1B, APP, SRC, ITGA4, RPA1 and ACTB were identified as key genes in the PPI network, miRNA-hub gene regulatory network and TF-hub gene regulatory network. TRIM54, ELAVL2, PTN, KIT, BMPR1B, APP, SRC, ITGA4, RPA1 and ACTB in GDM were validated using ROC analysis. This investigation provides further insights into the molecular pathogenesis of GDM, which might facilitate the diagnosis and treatment of GDM.

show abstract

CXCL5 Downregulation in Villous Tissue Is Correlated With Recurrent Spontaneous Abortion

Cited by 10 publications

References 59 publications

Spheroid formation induces chemokine production in trophoblast‐derived Swan71 cells

Spheroid formation induces chemokine production in trophoblast‐derived Swan71 cells

IL-23 Inhibits Trophoblast Proliferation, Migration, and EMT via Activating p38 MAPK Signaling Pathway to Promote Recurrent Spontaneous Abortion

Identification of differentially expressed genes and signaling pathways in gestational diabetes mellitus by integrated bioinformatics analysis

Contact Info

Product

Resources

About