CXCL1 expression in human decidua in vitro is mediated via the MAPK signalling cascade

Baston-Büst, Dunja Maria; Schanz, A.; Böddeker, S.J.; Altergot-Ahmad, Olga; Krüssel, Jan-Steffen; Rein, Daniel T.; Hess, Alexandra P.

doi:10.1016/j.cyto.2013.07.023

Cited by 18 publications

(15 citation statements)

References 35 publications

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“…CXC motif ligand 1 (CXCL1) seems to be an important chemokine during embryonic implantation, since its expression increases significantly after treating decidualized (d) endometrial stromal (EnS) cells with trophoblast conditioned media or coculture with first-trimester trophoblast explants [ 17 , 18 ]. We have previously shown the induction of CXCL1 expression by the embryo secreted factor IL-1 β and its mediation by MAPK signaling and activation of NF- κ B in dEnS confirming the data of Issa et al regarding regulation of CXCL1 via NF- κ B [ 19 , 20 ]. CXCL1 acts through its receptor CXC motif receptor 2 (CXCR2) and the coreceptor Sdc-1 [ 21 , 22 ].…”

Section: Introductionsupporting

confidence: 87%

“…St-T1 and KdS1 were decidualized and incubated with 1 μ g/mL tetracycline (Tet) for Sdc-1 kd induction (only KdS1 and dKdS1) after reaching 70% confluence and incubated with 10, 25, and 50 μ M MAPKK (MEK1/2) inhibitor (PD 98.059, Sigma-Aldrich) and 0.1, 0.5, 1, 5, 10, and 25 μ M JNK inhibitor (AEG 3482, Tocris, Bristol, UK) for 2 h at 37°C and 5% CO 2 followed by 48 h of incubation with 10 ng/mL IL-1 β for St-T1 (data not shown) and KdS1 and 0.1 ng/mL IL-1 β for dSt-T1 and dKdS1 at equal conditions [ 20 ]. Afterwards, the cell-culture media supernatants were harvested and analyzed regarding the CXCL1 expression by ELISA.…”

Section: Methodsmentioning

confidence: 99%

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Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

Baston-Buest

Altergot-Ahmad

Pour

et al. 2017

Mediators of Inflammation

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Successful implantation of the embryo into the human receptive endometrium is substantial for the establishment of a healthy pregnancy. This study focusses on the role of Syndecan-1 at the embryo-maternal interface, the multitasking coreceptor influencing ligand concentration, release and receptor presentation, and cellular morphology. CXC motif ligand 1, being involved in chemotaxis and angiogenesis during implantation, is of special interest as a ligand of Syndecan-1. Human endometrial stromal cells with and without Syndecan-1 knock-down were decidualized and treated with specific inhibitors to evaluate signaling pathways regulating CXC ligand 1 expression. Western blot analyses of MAPK and Wnt members were performed, followed by analysis of spheroid interactions between human endometrial cells and extravillous trophoblast cells. By mimicking embryo contact using IL-1β, we showed less ERK and c-Jun activation by depletion of Syndecan-1 and less Frizzled 4 production as part of the canonical Wnt pathway. Additionally, more beta-catenin was phosphorylated and therefore degraded after depletion of Syndecan-1. Secretion of CXC motif ligand 1 depends on MEK-1 with respect to Syndecan-1. Regarding the interaction of endometrial and trophoblast cells, the spheroid center-to-center distances were smaller after depletion of Syndecan-1. Therefore, Syndecan-1 seems to affect signaling processes relevant to signaling and intercellular interaction at the trophoblast-decidual interface.

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Section: Introductionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

Baston-Buest

Altergot-Ahmad

Pour

et al. 2017

Mediators of Inflammation

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“…The regulation network of EGFR in OC3 cells warrants further investigation. CXCL1 expression is markedly upregulated by MAPK/ERK signaling in human decidua [ 38 ]. This finding is consistent with that of our study, in which the intrinsic and IL-1β-induced-CXCL1 expression was abolished by the MAPK inhibitor U0126 in DOK and TW2.6 cells ( Supplementary Figure S3A ).…”

Section: Discussionmentioning

confidence: 99%

Interleukin-1 beta transactivates epidermal growth factor receptor via the CXCL1-CXCR2 axis in oral cancer

et al. 2015

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Hyperactivation of the epidermal growth factor receptor (EGFR) pathways and chronic inflammation are common characteristics of oral squamous cell carcinoma (OSCC). Previously, we reported that OSCC cells secrete interleukin-1 beta (IL-1β), which promotes the proliferation of the oral premalignant cell line, DOK, and stimulates DOK and OSCC cells to produce the chemokine CXCL1. CXCL1 functions through CXCR2, a G protein-coupled receptor that transactivates EGFR in ovarian and lung cancers. We hypothesized that IL-1β transactivates EGFR through the CXCL1–CXCR2 axis in OSCC. In this study, we demonstrated that tyrosine phosphorylation of EGFR is crucial for the IL-1β-mediated proliferation and subsequent bromodeoxyuridine (BrdU) incorporation of DOK cells because the EGFR inhibitors AG1478 and erlotinib inhibit these abilities in a dose-dependent manner. Addition of IL-1β instantly enhanced CXCL1 expression and secretion (within 15 min) in the DOK and OSCC cell lines. Furthermore, tyrosine phosphorylation of EGFR was significantly enhanced in DOK (1 h) and OSCC (20 min) cell lines after IL-1β treatment, and both cell lines were inhibited on the addition of an IL-1 receptor antagonist (IL-1Ra). CXCL1 treatment resulted in EGFR phosphorylation, whereas the knockdown of CXCL1 expression by lentivirus-mediated shRNA or the addition of the CXCR2 antagonist SB225002 dramatically reduced IL-1β-mediated EGFR phosphorylation and proliferation of DOK cells. Neutralizing antibodies against IL-1β or CXCL1 markedly inhibited the constitutive or IL-1β-induced tyrosine phosphorylation of EGFR in OSCC cells. IL-1β transactivates EGFR through the CXCL1-CXCR2 axis, revealing a novel molecular network in OSCC that is associated with autocrine IL-1β and EGFR signaling.

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“…We previously reported that GRO -α expres- We also investigated the signaling pathway involved in the regulation of GRO -α production in synovial fibroblasts following 30 kDa fibronectin fragment treatment. 30 kDa fibronectin fragmentstimulated GRO -α production was examined in the presence of MAPK and NFκB inhibitors because GRO -α is known to be regulated by IL -1β and TNF -α through MAPK and NFκB (26,27) . 30 kDa fibronectin fragmentstimulated GRO -α production was decreased by SP600125, SB203580 and APDC.…”

Section: Discussionmentioning

confidence: 99%

Effect of a 30 kDa Fibronectin Fragment on GRO Production by Human Temporomandibular Joint Synovial Fibroblasts

Suzuki

Ogura

2020

Int J Oral-Med Sci

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The human temporomandibular joint (TMJ) is a bilateral synovial joint formed from the upper temporal bone and mandible. The synovium exists at the surface of the soft connective tissue in the intra articular structures of the TMJ, excluding the cartilage of the articular eminence, mandibular fossa and head of mandible, and the articular disc (1). The lining layer of synovial tissue is populated by fibroblastlike cells and macrophagelike cells (2, 3). The loose connective tissue of the synovial sublining contains blood vessel sublining fibroblasts and leukocytes. These cells in the synovium play important roles in homeostasis and the progression of pathologic conditions (1). Synovitis, which is characterized by chronic inflammatory changes, such as growth of small new blood vessels and infiltration of inflammatory cells, often accompanies disk displacement (DD) /internal derangement (ID) and osteoarthritis (OA) of the temporomandibular joint (TMJ) and may cause TMJ pain (4, 5). Most inflammatory conditions of the TMJ are noninfectious inflammation, and excessive mechanical stress is considered one reason for the occurrence of inflammation in the TMJ (6). However, proinflammatory substances and the mechanisms causing inflammation in the TMJ are poorly understood.

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CXCL1 expression in human decidua in vitro is mediated via the MAPK signalling cascade

Cited by 18 publications

References 35 publications

Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

Interleukin-1 beta transactivates epidermal growth factor receptor via the CXCL1-CXCR2 axis in oral cancer

Effect of a 30 kDa Fibronectin Fragment on GRO Production by Human Temporomandibular Joint Synovial Fibroblasts

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