CuZn superoxide dismutase transgenic retinal transplants

Grasbon, Thomas; Grasbon-Frodl, E. M.; Juliusson, Bengt; Epstein, Charles J.; Brundin, Patrik; Ehinger, Berndt

doi:10.1007/s004170050241

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…Validation of pharmacological activity and anti-pollution effect of neem tree leaf 399 강한 hydroxy radical이 생성되는 것을 예방하는 작용을 하는 항염증 소재나 피부 노화방지를 위한 미용적인 소재 로 화장품 등과 같은 첨가제로 사용이 되고 있다 (Grasbon, 1999;Mckersie, 2000). SOD 유사활성 효과를 측정하여 님트리잎 추출물의 항산화 효과를 측정한 결과, 최고 농도 인 1,000 μg/mL 농도에서 24.13%의 유사활성을 보였다 (Fig.…”

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Validation of pharmacological activity and anti-pollution effect of ethanol extract of Azadirachta indica leaf

Hyun¹,

Lee²,

Kang³

et al. 2020

Korean J. Food Preserv.

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In this study, we investigated the anti-oxidant, anti-inflammatory, whitening, anti-wrinkle, and anti-pollution effects of Neem tree (i.e., Azadirachta indica leaf) 70% ethanol extract. More specifically, the polyphenol content was 51.37 mg/g, and the DPPH and ABTS radical scavenging activities were 34.09 and 72.18%, respectively, at a final concentration of 1,000 μg/mL. SOD experiments showed an effect of 24.13% at a final concentration of 1,000 μg/mL. In addition, to investigate the whitening effect, the tyrosinase inhibition effect was measured, giving a value of 47.21% at a final concentration of 1,000 μg/mL. Furthermore, MTT assay experiments were carried out on macrophages (RAW 264.7) to determine the cytotoxicity using the neem tree leaf extract, it was confirmed that the cell viability was unaffected up to a concentration of 100 μg/mL. Moreover, anti-inflammatory (NO) analysis of the cells using macrophages stimulated with LPS induced an inflammatory response of 68.86%, in addition to an anti-inflammatory activity of 66.45% at a final concentration of 100 μg/mL. The iNOS protein showed an inhibition rate of 97.49% at a concentration of 100 μg/mL, and in the cytotoxicity assay (MTT assay) using keratinocytes (HaCaT), the cell viability was similar to that of the untreated control at 10 μg/mL. Western blotting analysis confirmed the inhibition rate of MMP-1. Additionally, HaCaT and CCD-986sk human skin cells were stimulated with benzo[e]pyrene, which contains fine dust and heavy metals, and then treated with neem tree extract to measure the effect of the extract on the cell viability. Indeed, the anti-pollution efficacy of the extract was confirmed. Overall, the benzo[e]pyrene-stimulated cells presented improvements of >92 and 81% for the HaCaT and CCD cells at a concentration of 25 μg/mL, while improvements were observed at a concentration of 100 μg/mL HaCaT for fine dust-stimulated cells. Moreover, HaCaT cell and CCD cells showed improvements of more than 85 and 76%, respectively. The obtained results therefore indicate that Neem tree 70% ethanol extract is expected to have value for application in cosmetics due to its pharmacological activity and its fine dust-blocking properties.

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Validation of pharmacological activity and anti-pollution effect of ethanol extract of Azadirachta indica leaf

Hyun¹,

Lee²,

Kang³

et al. 2020

Korean J. Food Preserv.

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“…적송잎 추출물의 SOD 유사활성 항산화 효소 중의 하나인 SOD는 산소를 소비하는 모든 생물에 존재하며 대표적인 활성산소 저해제로 반응성이 매 우 강한 superoxide radical과 반응하여 hydrogen peroxide (H2O2) 생성을 촉매하는 효소이다. 가장 독성이 강한 hydroxyl radical의 생성을 예방하는 작용을 하여 현재 항염증 소재나 피부 노화방지를 위한 미용소재로 화장품 등의 첨가 제로서 사용되고 있다(31,32). 따라서 본 연구에서 superoxide 와 반응하여 갈변현상을 나타내는 pyrogallol의 자동산화 반 응 억제능을 측정하는 원리(29)를 이용하여, 적송잎 추출물 별 SOD 유사활성을 측정하였다(Fig.…”

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Effect of Pine (Pinus densiflora) Needle Extracts on Antioxidant Activity and Proliferation of Osteoclastic RAW 264.7 Cells

Jeon¹,

Park²,

Park³

et al. 2011

Journal of the Korean Society of Food Science and Nutrition

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Pine needles have long been used as a traditional health-promoting medicinal food in Korea. This study was carried out to investigate the effects of pine needle extracts on the antioxidant activity, and proliferation of osteoclastic RAW 264.7 cells. Pine needle extracts were examined using hot water, ethanol, hexane, hot water-ethanol, and hot water-hexane. The effects of the pine needle extracts were examined by comparing the results with that of a commercial agents, proanthocyanidin. Analysis of each extract indicated that hot water-ethanol and ethanol extracts contained the highest total polyphenol concentrations. The hot water-ethanol and ethanol extracts also showed relatively the highest SOD-like activity. The proliferation of osteoclastic RAW 264.7 cells treated with pine needle extracts was decreased by lower than 70%. In addition, the hot water and ethanol extracts of pine needle significantly reduced the number of tartrate-resistant acid phosphatase-positive (TRAP +) multinucleated cells from osteoclatic RAW 264.7 cells. These results indicate that pine needle extracts had an anabolic effect on bone through the promotion of osteoclast differentiation, suggesting that they could be used for the treatment of common metabolic bone diseases.

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Retinal Repair by Stem Cell Transplantation

Stern

Temple

2006

Stem Cell and Gene-Based Therapy

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CuZn superoxide dismutase transgenic retinal transplants

Abstract: Enhanced intracellular SOD levels do not seem to influence retinal transplant morphology as detected by light microscopy. However, volumes of the SOD transgenic transplants were found to be increased compared to control grafts.

Cited by 7 publications

References 36 publications

Validation of pharmacological activity and anti-pollution effect of ethanol extract of Azadirachta indica leaf

Validation of pharmacological activity and anti-pollution effect of ethanol extract of Azadirachta indica leaf

Effect of Pine (Pinus densiflora) Needle Extracts on Antioxidant Activity and Proliferation of Osteoclastic RAW 264.7 Cells

Retinal Repair by Stem Cell Transplantation

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