Cutting through host autofluorescence: fluorescence lifetime imaging microscopy for visualising intracellular bacteria in Symbiodiniaceae
Pranali Deore,
Sarah Jane Tsang Min Ching,
Douglas R. Brumley
et al.
Abstract:Photoperiodicity is key to the synchronization of life stages in Symbiodiniaceae,Breviolum minutumwhich harbors taxonomically diverse epi- and endosymbiotic bacteria. We examined influence of a light dark regime on the spatial association betweenB. minutumand bacteria. We employed a novel approach using combination of fluorescence lifetime imaging microscopy with fluorescence in situ hybridisation approach to clearly distinguish labelled intracellular bacteria from broad spectrum (450–800 nm) background autofl… Show more
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